Antibodies used for immunoblotting |
Antibody (Dilution) |
Lamin A |
ab26300, Abcam (1:1000) |
Progerin |
SAB4200272, Sigma-Aldrich (1:1000) |
Lamin A/C |
sc-20681, SCBT (1:2000) |
VDR |
sc-13133, SCBT (1:200) |
β-Tubulin |
T8238, Sigma Aldrich (1:2000) |
STAT1 |
#9172, Cell Signaling (1:1000) |
P-STAT1 (Y701) |
#7649, Cell Signaling (1:1000) |
P-STAT1 (S727) |
#9177, Cell Signaling (1:1000) |
P-RPA |
A300–245A, Bethyl Laboratories (1:1000) |
ISG15 |
sc-166755, SCBT (1:1000) |
cGAS |
#D1D3G, Cell Signaling (1:300) |
STING |
#D2P2F, Cell Signaling (1:1000) |
RAD51 |
#PC130, Calbiochem (1:1000) |
53BP1 |
#22760, SCBT (1:1000) |
Antibodies used for immunofluorescence |
Antibody |
P-RPA |
A300–245A, Bethyl Laboratories (1:1000) |
RAD51 |
#PC130, Calbiochem (1:1000) |
53BP1 |
#22760, SCBT (1:400) |