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. 2002 Nov 13;14(1):35–48. doi: 10.1016/0165-5728(87)90099-3

Chronic Theiler's virus infection in mice: appearance of myelin basic protein in the cerebrospinal fluid and serum antibody directed against MBP

Helene C Rauch 1,∗, Ilene Nowicki Montgomery 1, Channing L Hinman 2, Walid Harb 1, Joyce A Benjamins 3
PMCID: PMC7119459  PMID: 2433306

Abstract

Myelin basic protein (MBP) appears frequently in the cerebrospinal fluid (CSF) of mice with chronic demyelination following intracerebral infection with Theiler's murine encephalomyelitis virus (TMEV); antibody to MBP can frequently be found in the sera. The peaks of the immune responses to both MBP and TMEV coincide with the time course of the clinical signs of disease. Adsorption of mouse sera with TMEV or MBP indicate the non-identity of the antigens and the specificity of the antisera as measured by ELISA. Immunoblot analysis of sera confirmed the ELISA findings. The mechanism of induction of antibody directed against MBP and its role in TMEV-associated demyelination remain to be determined.

Keywords: Myelin basic protein, Theiler's murine encephalomyelitis virus, Cerebrospinal fluid, Anti-body against myelin basic protein (Mice)

Abbreviations: CNS, central nervous system; CSF, cerebrospinal fluid; EAE, experimental allergic encephalomyelitis; ic, intracerebrally; LODD, late-onset demyelinating disease; LMND, lower motor neuron disease; MBP, myelin basic protein; RR, righting reflex; TMEV, Theiler's murine encephalomyelitis virus

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