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. 2002 Nov 13;14(3):305–316. doi: 10.1016/0165-5728(87)90017-8

Analysis of the intrathecal humoral immune response in Brown Norway (BN) rats, infected with the murine coronavirus JHM

R Dörries 1,∗, R Watanabe 1, H Wege 1, V ter Meulen 1
PMCID: PMC7119463  PMID: 3031130

Abstract

Serum and CSF specimens from clinically healthy Brown Norway (BN) rats inoculated intracerebrally with corona virus JHM were analysed with respect to the state of the blood-brain barrier (BBB) and the intrathecal synthesis and isoelectric distribution of immunoglobulins (Ig). Increased CSF/serum ratios for Ig in the context of an intact BBB were never seen in the absence of intrathecal synthesis of virus-specific antibodies. Affinity-mediated immunoblot analysis revealed a broad pattern of virus-specific antibodies with embedded clusters of restricted heterogeneity, but no signs of oligoclonal Ig production carrying non-viral specificity. From these data it was concluded that BN rats do control the intracerebral spread of JHM virus effectively by a strong local virus-specific antibody response, thereby preventing a clinically apparent disease.

Keywords: (Brown Norway rat), Coronavirus JHM, Demyelination, Antibody synthesis, (Intrathecal), Isoelectric focusing

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