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. 1999 Mar 12;34(1):25–33. doi: 10.1016/0165-5876(95)01231-1

Interferon production by leukocytes in children with otitis media with effusion

Anne Pitkäranta a,b,∗, Tapani Hovi b, Pekka Karma a
PMCID: PMC7119466  PMID: 8770670

Abstract

We have previously shown that leukocyte cultures of children suffering from recurrent respiratory tract infections produce less interferon (IFN) than those of healthy children. In the present study this tentative marker of recurrent infections was used to study the pathogenetic background of otitis media with effusion (OME). Altogether 57 consecutive children, aged 2–11 years, who came for tympanostomy and/or adenoidectomy were divided into three subgroups: 25 of them had OME and a history of recurrent acute otitis media (rAOM/OME +), 20 had OME without an infectious background (inf- /OME +), and 12 had a history of recurrent upper respiratory infections (inf +/OME —) without OME. All the children were free of acute illness at the time of sampling. Differences between the groups were seen in IFN yields when leukocyte cultures were stimulated with adeno-, rhino-, corona-, respiratory syncytial or influenza A viruses. Leukocytes from inf- /OME + children produced more IFN than those of the other two groups. Though no sex differences in the IFN responses were seen among rAOM/OME + and inf + /OME- children, leukocytes from inf- /OME + girls produced significantly higher amounts of IFN than those of inf- /OME + boys, or rAOM/OME + and inf + /OME- children. These differences between clinically different groups of children support the view that the etiology of OME can be heterogeneous.

Keywords: Acute otitis media, Interferon, Otitis media with effusion, Respiratory infection, Secretory otitis media

References

  • 1.Arola M., Ziegler T., Puhakka H., Lehtonen O., Ruuskanen O. Rhinovirus in otitis media with effusion. Ann. Otol. Rhinol. Laryngol. 1990;99:451–453. doi: 10.1177/000348949009900607. [DOI] [PubMed] [Google Scholar]
  • 2.Bernstein J.M., Doyle W.J. Role of IgE-mediated hypersensitivity in otitis media with effusion: pathophysiologic considerations. Ann. Otol. Rhinol. Laryngol. 1994;103:15–19. doi: 10.1177/00034894941030s505. [DOI] [PubMed] [Google Scholar]
  • 3.Bondestam M., Alm G.V., Foucard T. Interferon production in children with undue susceptibility to infections. Acta Paediatr. Scand. 1984;73:197–202. doi: 10.1111/j.1651-2227.1984.tb09928.x. [DOI] [PubMed] [Google Scholar]
  • 4.Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Lab. Invest. 1968;21(Suppl 97):77–89. [PubMed] [Google Scholar]
  • 5.Bylander A. Upper respiratory tract infection and Eustachian tube function in children. Acta Otolaryngol. (Stockh.) 1984;97:343–349. doi: 10.3109/00016488409130998. [DOI] [PubMed] [Google Scholar]
  • 6.Cantell K., Strander H., Saxén L., Meyer B. Interferon response of human leukocytes during intrauterine and postnatal life. J. Immunol. 1968;100:1304–1309. [PubMed] [Google Scholar]
  • 7.Casselbrant M.L., Brostoff L.M., Cantekin E.I. Otitis media with effusion in preschool children. Laryngoscope. 1985;95:428–436. doi: 10.1288/00005537-198504000-00011. [DOI] [PubMed] [Google Scholar]
  • 8.Collins M.P., Church M.K., Bakshi K.N., Osborne J. Adenoid histamine and its possible relationship to secretory otitis media. J. Laryngol. Otol. 1985;99:685–691. doi: 10.1017/s0022215100097486. [DOI] [PubMed] [Google Scholar]
  • 9.Daly K. Risk factors for otitis media sequelae and chronicity. Ann. Otol. Rhinol. Laryngol. 1994;103:39–44. doi: 10.1177/00034894941030s511. [DOI] [PubMed] [Google Scholar]
  • 10.Johansson S.G.O., Berg T. Immunoglobulin levels in healthy children. Acta Paediatr. Scand. 1967;56:572–579. doi: 10.1111/j.1651-2227.1967.tb15982.x. [DOI] [PubMed] [Google Scholar]
  • 11.Karma P., Sipilä M. Occurrence of mucoid secretory otitis media up to the age of 12 years. Proceedings of the Second Extraordinary International Symposium on Recent Advances in Otitis Media; March 31–April 3; 1993. pp. 97–100. [Google Scholar]
  • 12.Karma P. Secretory otitis media-infectious background and its implications for treatment. Acta Otolaryngol. (Stockh.) 1987;449:47–48. doi: 10.3109/00016488809106372. [DOI] [PubMed] [Google Scholar]
  • 13.Linnavuori K. History of recurrent herpes correlates with relatively low Interferon production by herpes simplex virus induced cultured monocytes. J. Med. Virol. 1988;25:61–68. doi: 10.1002/jmv.1890250109. [DOI] [PubMed] [Google Scholar]
  • 14.Muller U., Steinhoff U., Reis L.F.L., Hemmi S., Pavlovic J., Zinkernagel R.M., Aguet M. Functional role of type I and type II interferons in antiviral defence. Science. 1994;264:1918–1921. doi: 10.1126/science.8009221. [DOI] [PubMed] [Google Scholar]
  • 15.Otten F.W.A., Grote J.J. Otitis media with effusion and chronic upper respiratory tract infection in children: A randomized, placebo-controlled clinical study. Laryngoscope. 1990;100:627–633. doi: 10.1288/00005537-199006000-00014. [DOI] [PubMed] [Google Scholar]
  • 16.Pitkäranta A., Linnavuori K., Roivainen M., Hovi T. Induction of interferon-α in human leukocytes by polioviruses: wild-type strains are better inducers than attenuated strains. Virology. 1988;165:476–481. doi: 10.1016/0042-6822(88)90591-0. [DOI] [PubMed] [Google Scholar]
  • 17.Pitkäranta A., Linnavuori K., Hovi T. Virus-induced interferon production in human leukocytes: a low responder to one virus can be a high responder to another virus. J. Inter. Res. 1991;11:17–23. doi: 10.1089/jir.1991.11.17. [DOI] [PubMed] [Google Scholar]
  • 18.Pitkäranta A., Karma P., Hovi T. Deficiency in interferon production by leukocytes from children with recurrent respiratory infections. Clin. Diag. Virol. 1993;1:101–108. doi: 10.1016/0928-0197(93)90017-Y. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Pukander J., Karma P., Sipilä M. Occurrence and recurrence of acute otitis media among children. Acta Otolaryngol. (Stockh.) 1982;94:479–486. doi: 10.3109/00016488209128938. [DOI] [PubMed] [Google Scholar]
  • 20.Ruuskanen O., Arola M., Heikkinen T., Ziegler T. Viruses in acute otitis media: increasing evidence for clinical significance. Pediatr. Infect. Dis. J. 1991;10:425–427. doi: 10.1097/00006454-199106000-00001. [DOI] [PubMed] [Google Scholar]
  • 21.Suetake M., Hozawa K., Takasaka T., Yuasa R., Ohyama K., Irimada M., Endo H., Shimoda H. In: Intravenous immunoglobulin therapy for otitis prone children. Infections in childhood. Sade, editor. Elsevier Science B.V; 1994. [Google Scholar]
  • 22.Teele D.W., Klein J.O., Rosner B. Epidemiology of otitis media during the first seven years of life in children in Greater Boston: a prospective cohort study. J. Inf. Dis. 1989;160:83–94. doi: 10.1093/infdis/160.1.83. [DOI] [PubMed] [Google Scholar]
  • 23.Wald E.R., Dashefsky B., Byers C., Guerra N., Taylor F. Frequency and severity of infections in day care. J. Pediatr. 1988;112:540–546. doi: 10.1016/s0022-3476(88)80164-1. [DOI] [PubMed] [Google Scholar]
  • 24.Yamanaka N., Fader H. Antibody response to outer membrane protein of mono-typeable Haemophilus influenzae in otitis-prone children. J. Pediatr. 1993;122:212–218. doi: 10.1016/s0022-3476(06)80115-0. [DOI] [PubMed] [Google Scholar]

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