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. 2002 Nov 12;18(2):153–157. doi: 10.1016/0166-0934(87)90120-0

Assessment of intestinal damage in rotavirus infected neonatal mice by a d-xylose absorption test

MK Ijaz 1,∗, MI Sahara 1,2, PJ Frenchick 1, LA Babiuk 1,2
PMCID: PMC7119480  PMID: 3429602

Abstract

A d-xylose absorption test has been standardized for use in newborn mice. It was used to measure small intestine dysfunction in neonate mice following infection with various isolates of rotaviruses. A xylose dose of 1 mg/g body weight was required to produce a maximum level of 100 μg/100 μl of plasma 2 h after administration of d-xylose. The mice inoculated with rotavirus absorbed significantly less d-xylose compared with uninoculated control mice. A micromethod is described which proved to be suitable to quantitate d-xylose for determination of absorptive function of the small intestine in small animals such as newborn mice.

Keywords: Animal model, Rotavirus, d-Xylose test

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