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. 2002 Nov 13;33(3):181–190. doi: 10.1016/0165-5728(91)90105-G

Mouse hepatitis virus (MHV-4, JHM) blocks γ-interferon-induced major histocompatibility complex class II antigen expression on murine cerebral endothelial cells

Jeymohan Joseph 1,∗, Robert L Knobler 1, Fred D Lublin 1, MN Hart 2
PMCID: PMC7119494  PMID: 1651958

Abstract

The regulation of γ-interferon-induced major histocompatibility complex (MHC) class II antigen expression on mouse cerebral endothelial cells by the neurotropic mouse hepatitis virus (MHV-4, JHM) was studied in vitro. The results presented demonstrate that MHV-4 can selectively block γ-interferon-induced class II antigen expression on cerebral endothelial cells. The blocking effect of class II expression occurs in a strain-dependent manner, and is limited to virus-susceptible mouse strains. Virus replication is not required to obtain the blocking effect since UV-inactivated MHV-4 produces the same result. MHV-4 blocking of γ-interferon-induced class II antigen expression is observed at both the cell surface (flow cytometry) and transcriptional level (Norhtern analysis).

Keywords: MHV-4, Major histocompatibility complex class II antigen, Interferon-γ, Cerebral endothelial cell, Flow cytometry, Northern analysis

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