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. 2002 Dec 9;29(1):89–103. doi: 10.1016/0165-2427(91)90055-H

Natural killer (NK) activity of porcine blood lymphocytes against allogeneic melanoma target cells

Mathias Büttner a, Rüdiger Wanke b, Bernd Obermann a
PMCID: PMC7119508  PMID: 1949586

Abstract

Allogeneic PM/86 melanoma cells of Munich Troll® miniature swine have been used for the demonstration of porcine peripheral blood NK cell activity. Compared with the specific lysis of xenogeneic K562-, U937- and Vero-target cells, NK cell-mediated cytotoxicity (NK-CMC) against PM/86 melanoma tumor cells was significantly lower in a 16 h chromium release assay. The target cell susceptibility to pheriperal blood NK-CMC of both adult Troll® miniature swine and German Landrace sows was very similar. Cold target inhibition assays revealed the allogeneic PM/86 melanoma cells to be the most powerful inhibitors of NK-CMC.

Nylon wool non-adherent lymphocytes produced interferon (IFN)-alpha in different quantities upon contact with NK susceptible target cells. The NK effector cells could lytic activity against all susceptible targets by a moderate dose of natural human inteleukin-2 (nhuIL-2).

The role of NK-CMC in melanoma tumor rejection and/or prevention of metastases is yet unknown in swine although porcine melanoma serves as a good model for the disease in man.

Abbreviations: FCS, fetal calf serum; IFN, interferon; LeuOMe, L-leucine methyl ester; MEM, minimal essential medium; MR, maximal release; nhuIL-2, natural human interleukin-2; NK, natural killer; NK-CMC, natural killer cell-mediated cytotoxicity; PBMC, peripheral blood mononuclear cells; PBS, phosphate-buffered saline; SPF, specific pathogen free; SR, spontaneous release; TNF, tumor necrosis factor; VSV, vesicular stomatitis virus

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