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. 2002 Nov 12;25(2):117–124. doi: 10.1016/0165-2427(90)90029-R

Recombinant porcine interferon-gamma activates in vitro porcine adherent mononuclear cells to produce interleukin 1

B Charley 1, Sophie Laverne 1, Laurence Lavenant 1
PMCID: PMC7119577  PMID: 2116051

Abstract

The effect of recombinant porcine interferon-gamma (rPoIFNγ) on in vitro production of interleukin 1 (IL-1) by porcine blood monocytes was studied. Three-day-old cultures of porcine adherent blood mononuclear cells were treated by doses of rPoIFNγ for 3 or 6 days before lipopolysaccharide (LPS)-induction. While rPoIFNγ alone had no effect, a combined treatment by rPoIFNγ and LPS enhanced the IL-1 secretory potential of adherent mononuclear cells and, to a lesser extent, the level of cell-associated IL-1. The IL-1 activity was neutralized by anti porcine IL-1 α and β antisera. These results demonstrate that rPoIFNγ has immunomodulatory effects in vitro on porcine monokine production.

References

  1. Arenzana-Seisdedos F., Virelizier J.L., Fiers W. Interferons as macrophage-activating factors. III. Preferential effects of interferon-γ on the interleukin 1 secretory potential of fresh or aged human monocytes. J. Immunol. 1985;134:2444–2448. [PubMed] [Google Scholar]
  2. Badger A.M., Meunier P.C., Weiss R.A., Bugelski P.J. Modulation of rat bronchoalveolar lavage cell function by the intratracheal delivery of interferon-γ. J. Interferon Res. 1988;8:251–260. doi: 10.1089/jir.1988.8.251. [DOI] [PubMed] [Google Scholar]
  3. Bielefeldt Ohmann H., Babiuk L.A. Alteration of some leukocyte functions following in vivo and in vitro exposure to recombinant bovine alpha and gamma interferon. J. Interferon Res. 1986;6:123–136. doi: 10.1089/jir.1986.6.123. [DOI] [PubMed] [Google Scholar]
  4. Bielefeldt Ohmann H., Lawman M.J.P., Babiuk L.A. Bovine interferon: its biology and application in veterinary medicine. Antiviral Res. 1987;7:187–210. doi: 10.1016/0166-3542(87)90028-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cavaillon J.M., Fitting C., Caroff M., Haeffner-Cavaillon N. Dissociation of cell-associated interleukin-1 (IL-1) and IL-1 release induced by lipopolysaccharide and lipid A. Infect. Immun. 1989;57:791–797. doi: 10.1128/iai.57.3.791-797.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Charley B., Leclerc C., Petit E., Chedid L. “In vitro” effects of lipopolysaccharide and mycobacterial cell wall components on swine alveolar macrophages. Res. Vet. Sci. 1983;34:212–217. doi: 10.1016/S0034-5288(18)32262-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Charley B., McCullough K., Martinod S. Antiviral and antigenic properties of recombinant porcine interferon gamma. Vet. Immunol. Immunopathol. 1988;19:95–103. doi: 10.1016/0165-2427(88)90001-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Czarniecki C.W., Hamilton E.B., Fennie C.W., Wolf R.L. In vitro biological activities of Escherichia coli derived bovine interferon α, β and γ. J. Interferon Res. 1986;6:29–37. doi: 10.1089/jir.1986.6.29. [DOI] [PubMed] [Google Scholar]
  9. Edwards C.K., Ghiasuddin S.M., Schepper J.M., Yunger L.M., Kelley K.W. A newly defined property of somatotropin: priming of macrophages for production of superoxyde anion. Science. 1988;230:769–771. doi: 10.1126/science.2829357. [DOI] [PubMed] [Google Scholar]
  10. Esparza I., Gonzales J.C., Vinuela E. Effect of interferon α, interferon γ and tumor necrosis factor on African swine fever virus replication in porcine monocytes and macrophages. J. Gen. Virol. 1988;69:2973–2980. doi: 10.1099/0022-1317-69-12-2973. [DOI] [PubMed] [Google Scholar]
  11. Russell S.W., Pace J.L. The effects of interferons on macrophages and their precursors. Vet. Immunol. Immunopathol. 1987;15:129–165. doi: 10.1016/0165-2427(87)90109-7. [DOI] [PubMed] [Google Scholar]
  12. Saklatvala J., Sarsfield S.J., Townsend Y. Pig interleukin 1. Purification of two immunologically different leukocyte proteins that cause cartilage resorption, lymphocyte activation, and fever. J. Exp. Med. 1985;162:1208–1222. doi: 10.1084/jem.162.4.1208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Saklatvala J., Sarsfield S.J., Wood D.D. An antiserum to pig IL-1 (catabolin) reacts with the acidic but not the neutral form of human IL-1. Br. J. Rheumatol. 1985;24(suppl. 1):68–71. [Google Scholar]
  14. Trinchieri G., Perussia B. Immune interferon: a pleiotropic lymphokine with multiple effects. Immunol. Today. 1985;6:131–136. doi: 10.1016/0167-5699(85)90080-5. [DOI] [PubMed] [Google Scholar]
  15. Virelizier J.L., Arenzana-Seisdedos F. Immunological functions of macrophages and their regulation by interferons. Med. Biol. 1985;63:149–159. [PubMed] [Google Scholar]
  16. Welsh R.M. Natural killer cells and interferon. CRC Crit. Rev. Immunol. 1984;5:55–93. [PubMed] [Google Scholar]

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