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. 2002 Nov 12;41(1):59–76. doi: 10.1016/0166-0934(93)90163-L

Genic amplification of the entire coding region of the HEF RNA segment of influenza C virus

Jean-Claude Manuguerra a,∗, Claude Hannoun a, Carolyn Nicolson b, James S Robertson b
PMCID: PMC7119587  PMID: 8381795

Abstract

In order to provide an easy and powerful analysis of influenza C viral HEF RNA segment of a recent strain, a combination of reverse transcription and the polymerase chain reaction was used. We amplified the entire coding region of the HEF gene of a laboratory strain of virus called C/Johannesburg/1/66, widely used for binding and esterase activity studies as well as that of a strain isolated in 1991 (C/Paris/145/91) from a patient suffering from severe flu syndrome. The sequences we amplified were about 2 kilobases long. In this work, we show that the forward ‘universal primer’ Unil, which has been used for influenza A and B viruses cDNA syntheses can also be used for influenza C virus. The PCR primers were designed to contain restriction sites to make the PCR products ready to be used for further purposes. A restriction analysis of the PCR products combined with analyses of all the human influenza C virus HEF gene sequences published so far permitted the design of sets of oligonucleotides which can prime PCR on cDNA of unknown influenza C virus for cloning.

Keywords: Influenza C virus, Reverse transcription, Polymerase chain reaction, Detection, HEF protein

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