Abstract
A number of viruses have been shown to be transmitted by the airborne route. It is the ability of these viruses to retain their infectivity for living hosts which play a key role in their aerial dissemination. Data generated by a number of workers on the airborne survival of viruses varies considerably because laboratory techniques have not been standardized. About 5 yr ago we started studies on the airborne survival of a number of animal and human viruses. This paper describes the methodology developed to study the aerobiology of these viruses. These methods should be useful in the aerobiological work of other viruses.
Keywords: Virus aerosol, Respiratory virus, Enteric virus, Viral aerobiological technique
References
- Adams D.J., Spendlove J.C., Spendlove R.S., Bennett B.B. Aerosol stability of infectious and potentially infectious reovirus particles. Appl. Environ. Microbiol. 1982;44:903–908. doi: 10.1128/aem.44.4.903-908.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Anderson A.A. New sampler for collection, sizing and enumeration of viable airborne particles. J. Bacteriol. 1958;76:471–484. doi: 10.1128/jb.76.5.471-484.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beard C.W., Easterday B.C. Aerosol transmission of hog cholera. Cornell Vet. 1965;55:630–636. [PubMed] [Google Scholar]
- Couch R.B., Cote T.R., Gerone P.J., Fleet W.F., Lang D.J., Griffith W.R., Knight V. Production of illness with a small particle aerosol of Coxsackie A 21. J. Clin. Invest. 1965;44:535–542. doi: 10.1172/JCI105166. [DOI] [PMC free article] [PubMed] [Google Scholar]
- De Jong J.C. Decay mechanisms of polio and EMC viruses in aerosols. In: Silver I.H, editor. Proc. Third Int. Symp. Aerobiology. Academic Press; New York: 1970. p. 210. [Google Scholar]
- De Jong J.C., Trouwborst T., Winkler K.C. The mechanism of virus decay in aerosols. In: Hers J.F.Ph., Winkler K.C., editors. Airborne Transmission and Airborne Infection. John Wiley and Sons; New York: 1973. pp. 124–130. [Google Scholar]
- Donaldson A.I. The influence of relative humidity on the aerosol stability of foot-and-mouth disease virus in aerosols from milk and fecal slurry. Res. Vet. Sci. 1973;15:96–101. [PubMed] [Google Scholar]
- Donaldson A.I., Ferris N.P. The survival of some airborne animal viruses in relation to relative humidity. Vet. Microbiol. 1976;1:413–420. [Google Scholar]
- Elazhary M.A.S.Y., Derbyshire J.B. Effect of temperature, relative humidity and medium on the aerosol stability of infectious bovine rhinotracheitis virus. Can. J. Comp. Med. 1977;43:158–167. [PMC free article] [PubMed] [Google Scholar]
- Elazhary M.A.S.Y., Derbyshire J.B. Aerosol stability of bovine parainfluenza type 3 virus. Can. J. Comp. Med. 1979;43:295–304. [PMC free article] [PubMed] [Google Scholar]
- Elazhary M.A.S.Y., Derbyshire J.B. Aerosol stability of bovine adenovirus type 3. Can. J. Comp. Med. 1979;43:305–312. [PMC free article] [PubMed] [Google Scholar]
- Goldberg L.J., Watkins H.M.S., Boerke E.E., Chatigny M.A. The use of a rotating drum for the study of aerosols over extended periods of time. Am. J. Hyg. 1958;68:85–93. doi: 10.1093/oxfordjournals.aje.a119954. [DOI] [PubMed] [Google Scholar]
- Guerin L.F., Mitchell C.A. A method for determining concentration of airborne virus and sizing droplet nuclei containing the agent. Can. J. Comp. Med, Vet. Sci. 1964;28:283–287. [PMC free article] [PubMed] [Google Scholar]
- Harper G.J. Airborne microorganisms: survival tests with four viruses. J. Hyg. 1961;59:479–486. doi: 10.1017/s0022172400039176. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harper G.J. The influence of environment on the survival of airborne virus particles in the laboratory. Arch. Gesamte Virusforsch. 1963;13:64–71. doi: 10.1007/BF01243824. [DOI] [PubMed] [Google Scholar]
- Hemmes J.H., Winkler K.C., Kool S.M. Virus survival as a seasonal factor in influenza and poliomyelitis. Nature (London) 1960;188:430–431. doi: 10.1038/188430a0. [DOI] [PubMed] [Google Scholar]
- Ijaz M.K., Sattar S.A., Johnson-Lussenburg C.M., Springthorpe V.S. Airborne survival of rotaviruses and coronaviruses: implications for disease transmission. In: Della-Porta A.J., editor. Virus Diseases of Veterinary Importance in South-East Asia and the Western Pacific. Academic Press; Sydney, Australia: 1984. [Google Scholar]
- Ijaz M.K., Sattar S.A., Johnson-Lussenburg C.M., Springthorpe V.S. Comparison of the airborne survival of calf rotavirus and poliovirus type 1 (Sabin) aerosolized as a mixture. Appl. Environ. Microbiol. 1985;49:289–293. doi: 10.1128/aem.49.2.289-293.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ijaz M.K., Sattar S.A., Johnson-Lussenburg C.M., Springthorpe V.S. Effect of relative humidity, atmospheric temperature and suspending medium on the airborne survival of human rotavirus. Can. J. Microbiol. 1985;31:681–685. doi: 10.1139/m85-129. [DOI] [PubMed] [Google Scholar]
- Ijaz M.K., Brunner A.H., Sattar S.A., Nair R.C., Johnson-Lussenburg C.M. Survival characteristics of airborne human coronavirus 229E. J. Gen Virol. 1985;66:2743–2748. doi: 10.1099/0022-1317-66-12-2743. [DOI] [PubMed] [Google Scholar]
- Ijaz M.K. Univ. of Ottawa; Ottawa, Ontario, Canada: 1985. Studies on the airborne survival of rotaviruses and a human coronavirus. (Ph.D. Thesis). [Google Scholar]
- Ijaz M.K., Sattar S.A. Spread of viral infections by aerosols. CRC Crit. Rev. Environ. Control. 1987;17:89–131. [Google Scholar]
- Kapikian A.Z., Conant R.M., Hamparian V.V., Chanock R.M., Dick E.C., Gwaltney J.M., Jr., Hamre D., Jordan W.S., Jr., Kenny G.E., Lennette E.H., Melnick J.L., Mogabab W.J., Phillips C.A., Schieble J.H., Tyrrell D.A.J. A collaborative report: Rhinoviruses — extension of the numbering system. Virol. 1971;43:524–526. [Google Scholar]
- Karim Y.G., Ijaz M.K., Sattar S.A., Johnson-Lussenburg C.M. Effect of relative humidity on the airborne survival of rhinovirus-14. Can. J. Microbiol. 1985;31:1058–1061. doi: 10.1139/m85-199. [DOI] [PubMed] [Google Scholar]
- Kennedy D.A., Johnson-Lussenburg C.M. Isolation and morphology of the internal component of human coronavirus, strain 229E. Intervirology. 1975/1976;6:197–206. doi: 10.1159/000149474. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knight V. Airborne transmission and pulmonary deposition of respiratory viruses. In: Knight V., editor. Viral and Mycoplasma Infections of the Respiratory Tract. Lea and Febiger; Philadelphia, PA: 1973. pp. 1–9. [Google Scholar]
- Lawless J.F. Statistical Models and Methods for Lifetime Data. John Wiley and Sons; New York: 1982. [Google Scholar]
- May K.R. The Collison nebulizer: description, performance and application. J. Aerosol. Sci. 1973;40:235–243. [Google Scholar]
- Miller W.S., Artenstein M.S. Vol. 125. 1967. Aerosol stability of 3 acute respiratory disease viruses; pp. 222–227. (Proc. Soc. Exp. Biol. Med.). [DOI] [PubMed] [Google Scholar]
- Ramia S., Sattar S.A. Simian rotavirus SA-11 plaque formation in the presence of trypsin. J. Clin. Microbiol. 1979;10:609–614. doi: 10.1128/jcm.10.5.609-614.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sattar S.A., Ijaz M.K., Johnson-Lussenburg C.M., Springthorpe V.S. Effect of relative humidity on the airborne survival of rotavirus SA-11. Appl. Environ. Microbiol. 1984;47:879–881. doi: 10.1128/aem.47.4.879-881.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Songer J.R. Influence of relative humidity on the survival of some airborne viruses. Appl. Microbiol. 1967;15:35–42. doi: 10.1128/am.15.1.35-42.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spendlove J.C., Fannin K.F. Methods of characterization of virus aerosols. In: Gerba C.P., Goyal S.M., editors. Methods in Environmental Virology. Marcel Dekker Inc; New York: 1982. pp. 261–329. [Google Scholar]
- Trouwborst T., De Jong J.C. Interaction of some factors in the mechanism of inactivation of bacteriophage MS2 in aerosols. Appl. Microbiol. 1973;26:252–257. doi: 10.1128/am.26.3.252-257.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tyler M.E., Shipe E.L. Bacterial aerosol samples. I. Development and evaluation of the All Glass Impinger. Appl. Microbiol. 1959;7:337–349. doi: 10.1128/am.7.6.337-349.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wyatt R.G., James W.D., Bohl E.H., Theil K.W., Saif L.J., Kalica A.R., Greenberg H.B., Kapikian A.Z., Chanock R.M. Human rotavirus type 2: cultivation in vitro. Science. 1980;207:189–191. doi: 10.1126/science.6243190. [DOI] [PubMed] [Google Scholar]