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. 1998 Jan 5;64(2):125–130. doi: 10.1016/S0166-0934(96)02151-9

Rapid diagnosis of avian infectious bronchitis virus by the polymerase chain reaction

Emiliana Falcone 1, Emanuela D'Amore 1, Livia Di Trani 1, Amalia Sili 1, Maria Tollis 1,∗
PMCID: PMC7119601  PMID: 9079758

Abstract

A simple, sensitive and specific polymerase chain reaction (PCR) procedure was developed in order to detect infectious bronchitis virus (IBV) directly in tissue samples. Viral RNA was extracted from allantoic fluids and cell cultures infected experimentally with different strains of IBV and from tissues of naturally infected birds. Viral RNA was then amplified and identified by a nested RT-PCR assay using two sets of primers flanking a well-conserved region of the nucleocapsid gene. The selected IBV nucleocapsid sequence was detected successfully by simple direct electrophoresis of amplified material.

Keywords: Infectious Bronchitis Virus, Polymerase Chain Reaction, Diagnosis

References

  1. Andreasen J.R., Jr., Jackwood M.W., Hilt D.A. Polymerase Chain Reaction amplification of the genome of infectious bronchitis virus. Avian Dis. 1991;35:216–220. [PubMed] [Google Scholar]
  2. Belak S., Ballagi-Pordany A. Application of the Polymerase Chain Reaction (PCR) in veterinary diagnostic virology. Vet. Res. Commun. 1993;17:55–72. doi: 10.1007/BF01839180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boursnell M.E.G., Binns M.M., Brown T.D.K. Vol. 5. Basel; Karger: 1989. Molecular biology of avian infectious bronchitis virus; pp. 65–82. (Prog. Vet. Microbiol. Immunol.). [PubMed] [Google Scholar]
  4. Boursnell M.E.G., Binns M.M., Foulds I.J., Brown T.D.K. Sequences of the nucleocapsid genes from two strains of avian infectious bronchitis virus. J. Gen. Virol. 1985;66:573–580. doi: 10.1099/0022-1317-66-3-573. [DOI] [PubMed] [Google Scholar]
  5. Capua I., Gough R.E., Mancini M. A ‘novel’ infectious bronchitis strain infecting broiler chickens in Italy. J. Vet. Med. B. 1994;41:83–89. doi: 10.1111/j.1439-0450.1994.tb00211.x. [DOI] [PubMed] [Google Scholar]
  6. Chou Q., Russel M., Birch D.E. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res. 1992;20(7):1717–1723. doi: 10.1093/nar/20.7.1717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cunningham C.H. Avian infectious bronchitis. Adv. Vet. Sci. Comp. Med. 1970;14:105–148. [PubMed] [Google Scholar]
  8. Gelb J., Jr., Wolff J.B., Moran C.A. Variant serotypes of infectious bronchitis virus isolated from commercial layer and broiler chickens. Avian Dis. 1991;35:82–87. [PubMed] [Google Scholar]
  9. Jackwood M.W., Kwon H.M., Hilt D.A. Infectious bronchitis virus detection in allantoic fluid using the Polymerase Chain Reaction and a DNA probe. Avian Dis. 1992;36:403–409. [PubMed] [Google Scholar]
  10. Jia W., Karaca K., Parrish C.R., Naqi S.A. A novel variant of avian infectious bronchitis virus resulting from recombination among three different strains. Arch. Virol. 1995;140:259–271. doi: 10.1007/BF01309861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. King D.J., Cavanagh D. Infectious bronchitis. In: Calnek B.W., Barnes H.J., Beard C.W., editors. Disease of poultry. 9th edn. Iowa State University Press; Ames, IA: 1991. pp. 485–495. [Google Scholar]
  12. King D.J. Identification of recent infectious bronchitis virus isolates that are serologically different from current vaccine strains. Avian Dis. 1988;32:362–364. [PubMed] [Google Scholar]
  13. Kwon H.M., Jackwood M.W., Brown T.P., Hilt D.A. Polymerase Chain Reaction and a biotin-labeled DNA probe for detection of infectious bronchitis virus in chickens. Avian Dis. 1993;37:149–156. [PubMed] [Google Scholar]
  14. Lukert P.D. Infectious bronchitis. In: Hitchner S.B., editor. Isolation and identification of avian pathogens. Arnold Printing; Ithaca, NY: 1975. [Google Scholar]
  15. Mullis K.B., Faloona F.A. Specific synthesis of DNA in Vitro via a Polymerase-Catalysed Chain Reaction. Methods Enzymol. 1987;155:334–350. doi: 10.1016/0076-6879(87)55023-6. [DOI] [PubMed] [Google Scholar]
  16. Office International des Epizooties (O.I.E.) Manual of standards for diagnostic tests and vaccines. Avian Infectious Bronchitis. 1992:549–561. 2nd edn. [Google Scholar]
  17. Pascucci S., Franciosi C. Epidemiologia della bronchite infettiva in Italia. Zootecnica International, Gennaio. 1991:68–73. [Google Scholar]
  18. Saiki R.K., Scharf S., Faloona F. Enzymatic amplification of B-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985;230:1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]
  19. Sutou S., Sato S., Okabe T. Cloning and sequencing of genes encoding structural proteins of avian infectious bronchitis virus. Virology. 1988;165:589–595. doi: 10.1016/0042-6822(88)90603-4. [DOI] [PubMed] [Google Scholar]
  20. Zwaagstra K.A., van der Zeijst B.A.M., Kusters J.G. Rapid detection and identification of avian infectious bronchitis virus. J. Clin. Microbiol. 1992;30(1):79–84. doi: 10.1128/jcm.30.1.79-84.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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