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. 2002 Nov 12;14(3):335–343. doi: 10.1016/0166-0934(86)90035-2

Two enzyme immunoassays for the detection of antibody to rodent coronaviruses

Abigail L Smith 1,2, Deborah F Winograd 1
PMCID: PMC7119637  PMID: 3025244

Abstract

Two enzyme immunoassays for detection of antibody to rodent coronaviruses were compared. Mouse hepatitis virus (MHV), strain JHM, antigen was in the form of formalin-fixed, infected 17 C1 1 cells. This antigen detected antibody to the homologous strain of MHV as well as to two heterologous MHV strains and a serologically related rat coronavirus, sialodacryodenititis virus. Antibody titers in assays using horseradish peroxidase (HRP)-conjugated or ureiase-conjugated anti-mouse IgG were substantially higher than in an indirect immunofluorescence assay. The ureiase assay was somewhat more sensitive than the HRP assay. MHV-JHM antigen was stable under a variety of storage conditions for at least two months.

Keywords: rodent coronavirus, mouse hepatitis virus, sialodacryoadenitis virus, enzyme immunoassay, horse-radish peroxidase, ureiase

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