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. 2002 Nov 13;27(2):89–97. doi: 10.1016/0165-5728(90)90058-U

Human oligodendrocytes are susceptible to cytolysis by major histocompatibility complex class I-restricted lymphocytes☆

Theodora CG Ruijs 1, Mark S Freedman 1, Yannick G Grenier 1, André Olivier 1, Jack P Antel 1,∗
PMCID: PMC7119690  PMID: 1970580

Abstract

The majority of human oligodendrocytes in enriched glial cell cultures expresses class I major histocompatibility complex (MHC) antigens. We used a 51Cr release assay to study the susceptibility of oligodendrocyte-enriched glial cells to MHC-restricted and non-restricted immune-mediated cytolysis. Mitogen-activated mononuclear cells induced significant lysis in a lectin-dependent cytotoxicity assay. Mononuclear cells allo-activated in a one-way mixed lymphocyte culture with E− cells from the glial cell donor induced a significantly higher degree of oligodendrocyte cytolysis than mononuclear cells activated with E− cells bearing MHC-class I antigens discordant with the glia. Cytolysis by alloactivated unfractionated lymphocytes and by purified CD8+ lymphocytes was reduced by an anti-class I antibody (W6/32). Our findings suggest that human oligodendrocytes can be susceptible targets for MHC class I-restricted lysis.

Keywords: Oligodendrocyte, Cytotoxic lymphocyte, Multiple sclerosis, (Human)

Footnotes

☆

This work was supported by grants from the Medical Research Council and the Multiple Sclerosis Society of Canada. Dr. Theodora Ruijs and Dr. Mark Freedman are Clinical and Research Fellows supported by the Medical Research Council of Canada.

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