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. 1998 Jan 5;58(1):17–26. doi: 10.1016/S0165-2427(96)05766-2

Elevated interleukin 6 activity in aqueous humor of cats with uveitis

Michael R Lappin a,∗, Steven W Dow b, John S Reif b, Matthew J Chavkin c
PMCID: PMC7119704  PMID: 9343336

Abstract

The purpose of this study was to assess the role of interleukin 6 (IL-6) in feline uveitis by measuring IL-6 activity in the serum and aqueous humor of cats.

Serum and aqueous humor was collected from clinically normal, random source cats (n = 10); clinically normal, specific-pathogen free cats experimentally inoculated with Toxoplasma gondii strain ME49 and sampled sequentially for 20 months (n = 4); and client-owned cats with uveitis (n = 27). Interleukin 6 activity was measured in each sample. Client-owned cats with uveitis were also evaluated for evidence of present or prior exposure to T. gondii, feline leukemia virus, feline immunodeficiency virus, and feline coronaviruses.

Interleukin 6 activity was non-detectable or low in serum from cats of each group. Interleukin 6 activity was not detected in aqueous humor of clinically normal cats. Interleukin 6 activity was detected in 2227 (81.5%) aqueous humor samples from cats with uveitis, with a range of 28.9 U ml−1-15702.9 U ml−1 (mean = 1911.9 U ml−1, SD = 3946.7 U ml−1). Serologic evidence of exposure to T gondii, feline immunodeficiency virus, feline leukemia virus, or a coronavirus was present in 2127 (77.8%) cats with uveitis. Interleukin 6 was detected in the aqueous humor of 1821 (85.7%) and 36 (50%) of the cats with and without serologic evidence of exposure to one to the infectious diseases, respectively. Statistically significant increases in mean IL-6 activity in aqueous humor were found for cats with any evidence of infection with T. gondii, for cats with T. gondii antigen in aqueous humor and for cats with coronavirus antibody titers ≥ 1:100. Aqueous humor IL-6 activity was greater than corresponding serum IL-6 activity in 2127 cats.

These results show that IL-6 is produced intraocularly in some cats with uveitis and that IL-6 may be a mediator of uveitis in cats.

Keywords: Toxoplasma gondii, Toxoplasmosis, Feline, Uveitis, Interleukin 6

References

  1. Beaman M.H., Hunter C.A., Remington J.S. Enhancement of intracellular replication of Toxoplasma gondii by IL-6: interactions with IFN-gamma and TNF-alpha. J. Immunol. 1994;153:4583–4587. [PubMed] [Google Scholar]
  2. Chardes T., Veige-Roussel F., Merelee P., Mevelec M.N., Buzoina-Gatel D., Bout D. Mucosal and systemic cellular immune responses induced by Toxoplasma gondii antigens in cyst orally infected mice. Immunology. 1993;78:421–429. [PMC free article] [PubMed] [Google Scholar]
  3. Charkin M.J., Lappin M.R., Poweli C.C., Roberts S.M., Parshall C.J., Reif J.S. Seroepidemiologic and clinical observations of 93 cases of uveitis in cats. Prog. Vet. Comp. Ophthalmol. 1992;2:29–36. [Google Scholar]
  4. Chavkin M.J., Lappin M.R., Powell C.C., Cooper C.M., Munana K.R. Toxoplasma gondii-specific antibodies in the aqueous humor of cats with toxoplasmosis. Am. J. Vet. Res. 1994;55:1244–1249. [PubMed] [Google Scholar]
  5. Davidson M.G., Nasisse M.P., English R.V., Wilcock B.P., Jamieson V.E. Feline anterior uveitis: a study of 53 cases. J. Am. Anim. Hosp. Assoc. 1990;27:77–83. [Google Scholar]
  6. de Vos A.F., Hoekzema R., Kijlstra A. Cytokines and uveitis, a review. Curr. Eye Res. 1992;6:581–597. doi: 10.3109/02713689209001814. [DOI] [PubMed] [Google Scholar]
  7. de Vos A.F., van Haren M.A.C., Verhagen C., Hoekzema R., Kijlstra A. Kinetics of intraocular tumor necrosis factor and interleukin-6 in endotoxin-induced uveitis in the rat. Invest. Ophthalmol. Vis. Sci. 1994;35:1100–1106. [PubMed] [Google Scholar]
  8. Dubey J.P., Carpenter J.L. Histologically confirmed clinical toxoplasmosis in cats: 100 cases (1952–1990) J. Am. Vet. Med. Assoc. 1993;203:1556–1566. [PubMed] [Google Scholar]
  9. Elmslie R.E., Ogilvie G.K., Dow S.W. Evaluation of a biologic response modifier derived from Serratia marcescens: effects on feline macrophages and usefulness for the prevention and treatment of viremia in feline leukemia virus-infected cats. Mol. Biother. 1991;3:231–238. [PubMed] [Google Scholar]
  10. English R.V., Davidson M.G., Nasisse M.R., Jamieson V.E., Lappin M.R. Intraocular disease associated with feline immunodeficiency virus infection in cats. J. Am. Vet. Med. Assoc. 1990;196:1116. [PubMed] [Google Scholar]
  11. Gilgor T., Andus T., Klapproth J., Hirano T., Kishimoto T., Heinrich P.C. Induction of rat acute-phase proteins by interleukin 6 in vivo. Eur. J. Immunol. 1988;18:717–721. doi: 10.1002/eji.1830180510. [DOI] [PubMed] [Google Scholar]
  12. Goitsuka R., Ohashi T., Ono K., Yasukawa K., Doishibara Y., Fukui H., Ohsugi Y., Hasegawa A. IL-6 activity in feline infectious peritonitis. J. Immunol. 1990;144:2599–2603. [PubMed] [Google Scholar]
  13. Hansen M.B., Nielson S.E., Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J. Immunol. Methods. 1989;119:203–206. doi: 10.1016/0022-1759(89)90397-9. [DOI] [PubMed] [Google Scholar]
  14. Hoekzema R., Murray P.I., Van Haren M.A.C., Helle M., Kijlstra A. Analysis of interleukin-6 in endotoxin-induced uveitis. Invest. Ophthalmol. Vis. Sci. 1991;32:88–95. [PubMed] [Google Scholar]
  15. Hunter C.A., Roberts C.W., Murray M., Alexander J.A. Detection of cytokine mRNA in the brains of mice with toxoplasmic encephalitis. Parasite Immunol. 1992;14:405–413. doi: 10.1111/j.1365-3024.1992.tb00015.x. [DOI] [PubMed] [Google Scholar]
  16. Hunter C.A., Roberts C.W., Alexander J. Kinetics of cytokine mRNA production in the brains of mice with progressive toxoplasmic encephalitis. Eur. J. Immunol. 1992;22:2317–2322. doi: 10.1002/eji.1830220921. [DOI] [PubMed] [Google Scholar]
  17. Kauffmann D.J.H., van Meurs J.C., Mertens D.A.E., Peperkamp E., Master C., Gerritsen M.E. Cytokines in vitreous humor: interleukin-6 is elevated in proliferative vitreoretinopathy. Invest. Ophthalmol. Vis. Sci. 1994;35:900–906. [PubMed] [Google Scholar]
  18. Kishimoto T. The biology of interleukin-6. Blood. 1989;74:1–10. [PubMed] [Google Scholar]
  19. Kunimoto D.Y., Nordan R.P., Strober W. IL-6 is a potent cofactor of IL-1 in 1gM synthesis and of IL-5 in IgA synthesis. J. Immunol. 1989;143:2230–2235. [PubMed] [Google Scholar]
  20. Lappin M.R., Roberts S.M., Davidson M.G., Powell C.C., Reif J.S. Enzyme-linked immunosorbent assays for the detection of Toxoplasma gondii-specific antibodies and antigens in the aqueous humor of cats. J. Am. Vet. Med. Assoc. 1992;201:1010–1016. [PubMed] [Google Scholar]
  21. Lappin M.R., Gasper P.W., Rose B.J., Powell C.C. Effect of primary phase feline immunodeficiency virus infection on cats with chronic toxoplasmosis. Vet. Immunol. Immunopathol. 1992;35:121–131. doi: 10.1016/0165-2427(92)90125-a. [DOI] [PubMed] [Google Scholar]
  22. Murray P.I., Hoekzema R., van Haren M.A.C., de Hon F.D., Kijlstra A. Aqueous humor interleukin-6 levels in uveitis. Invest. Ophthalmol. Vis. Sci. 1990;31:917–920. [PubMed] [Google Scholar]
  23. Norose K., Yano A., Wang X.C. Dominance of activated T cells and interleukin-6 in aqueous humor in Vogt-Koyanagi-Harada disease. Invest. Ophthalmol. Vis. Sci. 1994;35:33–39. [PubMed] [Google Scholar]
  24. Peiffer R.L., Wilcock B.P. Histopathologic study of uveitis in cats: 139 cases (1978–1988) J. Am. Vet. Med. Assoc. 1991;198:135–138. [PubMed] [Google Scholar]
  25. Post I.E., Wellenstein R.C., Clarke R.D. Proceedings, 21st Ann. Meet. Am. Assoc. Vet. Lab. Diagnost. 1978. Serologic test for feline infectious peritonitis using TGE virus antigen; pp. 427–436. [Google Scholar]
  26. Schluter D., Deckert-Schluter M., Schwendemann G., Brunner H., Hof H. Expression of major histocompatibility complex class II antigens and levels of interferongamma, tumour necrosis factor, and interleukin-6 in cerebrospinal fluid and serum in Toxoplasma gondii-infected SCID and immunocompetent C.B-17 mice. Immunology. 1993;78:430–435. [PMC free article] [PubMed] [Google Scholar]
  27. van der Lelij A., Rothova A., De Vries J.P., Vetter J.C.M., Van Haren M.A.C., Stilma J.S., Kijlstra A. Analysis of aqueous humour in ocular onchocerciasis. Current Eye Res. 1991;10:169–175. doi: 10.3109/02713689109001745. [DOI] [PubMed] [Google Scholar]
  28. van Snick J., Cayphass, Umik A. Vol. 83. 1986. Purification of NH2-terminal amino acid sequence of a T-cell derived cytokine with growth factor activity for B-cell hybridomas; pp. 9679–9683. (Proc. Natl. Acad. Sci. USA). [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Williams D. Local and systemic implications of feline ocular disease: iridal lesions. Fel. Pract. 1994;22:22–30. [Google Scholar]

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