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. 2002 Nov 11;40(1):45–56. doi: 10.1016/0166-0934(92)90006-Y

Blocking ELISA for distinguishing infectious bovine rhinotracheitis virus (IBRV)-infected animals from those vaccinated with a gene-deleted marker vaccine

Saul Kit a,∗, Haruki Otsuka a, Malon Kit b
PMCID: PMC7119712  PMID: 1331160

Abstract

A sensitive and specific blocking enzyme-linked immunosorbent assay (ELISA) was developed to distinguish infectious bovine rhinotracheitis virus (IBRV)-infected animals from those immunized with a glycoprotein gIII deletion mutant, IBRV(NG)dltkdlgIII. For this ELISA, undiluted test sera are used to block the binding of an anti-IBRV gIII monoclonal antibody (mAbgIII)-horseradish peroxidase (HRPO) conjugate to gIII antigen. TMB substrate is used for color development. Negative S/N values (defined as the absorbance at 650 nm of test sera/absorbance at 650 nm of negative control sera) of >0.80 were obtained with immune sera from gnotobiotic cattle immunized with several bovine viruses, with bovine antisera to bovine herpesvirus-2, and vesicular stomatitis virus, with porcine antisera to pseudorabies virus and parvovirus, and with normal sera from heterologous species. Negative S/N values were also obtained with sera from rabbits twice vaccinated with IBRV(NG)dltkdlgIII. However, the S/N values became positive (S/N <0.8) 10 to 17 days after the rabbits were challenge exposed to virulent IBRV(Cooper). Most of 116 sera (84%) from feedlot cattle with virus neutralization (VN) titers of < 1:2 or < 1:4 had negative S/N values >0.8, but 18 sera with negative VN titers had positive S/N values, consistent with observations indicating that an IBRV outbreak was occurring in one of the feedlot herds. Thirty nine sera (98%) from feedlot cattle with VN titers of 1:2 to 1:128 had positive S/N values (<0.8). One serum with a VN titer of 1:2 had a borderline (±) S/N value of 0.81. After immunization with a commercial gIII-positive IBRV vaccine, 115116 sera with VN titers of 1:2 to 1:256 had positive S/N values (<0.8). One serum with a VN titer of 1:2 had a negative S/N value of 0.83. Serum from one vaccinated animal that failed to seroconvert after vaccination (VN < 1:4) showed a strongly positive ELISA S/N of 0.48.

Keywords: Infections bovine rhinotracheitis virus, Bovine herpesvirus-1, Deletion mutants, Vaccine, Thymidine kinase, Glycoprotein gIII

References

  1. Ackermann M., Belak S., Bitsch V., Edwards S., Moussa A., Rockborn G., Thiry E. Round table on infectious bovine rhinotracheitis/infectious pustular vulvovaginitis virus infection diagnosis and control. Vet. Microbiol. 1990;23:361–363. doi: 10.1016/0378-1135(90)90167-t. [DOI] [PubMed] [Google Scholar]
  2. Ackermann M., Muller H.K., Bruckner L., Kihm U. Eradication of infectious bovine rhinotracheitis in Switzerland: review and prospects. Vet. Microbiol. 1990;23:365–370. doi: 10.1016/0378-1135(90)90168-u. [DOI] [PubMed] [Google Scholar]
  3. Babiuk L.A., L'Italien J., van Drunen Littel-van den Hurk S., Zamb T., Lawman M.J.P., Hughes G., Gifford G.A. Protection of cattle from bovine herpesvirus type I (BHV-1) infection by immunization with individual viral glycoproteins. Virology. 1987;159:57–66. doi: 10.1016/0042-6822(87)90347-3. [DOI] [PubMed] [Google Scholar]
  4. Bolton D.C., Chu H.J., Ardans A.A., Kelly B., Zee Y.C. Evaluation of the critical parameters of a sensitive Elisa test using purified infectious bovine rhinotracheitis virus antigens. Vet. Microbiol. 1981;6:265–279. [Google Scholar]
  5. Collins J.K., Bulla G.A., Riegel C.A., Butcher A.C. A single dilution enzyme-linked immunosorbent assay for the quantitative detection of antibodies to bovine herpesvirus type 1. Vet. Microbiol. 1984/1985;10:133–147. doi: 10.1016/0378-1135(85)90015-x. [DOI] [PubMed] [Google Scholar]
  6. Collins J.K., Butcher A.C., Teramoto Y.A., Winston S. Rapid detection of bovine herpesvirus type 1 antigens in nasal swab specimens with an antigen capture enzyme-linked immunosorbent assay. J. Clin. Microbiol. 1985;21:375–380. doi: 10.1128/jcm.21.3.375-380.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cook C.G., Letchworth G.J., Splitter G.A. Bovine naturally cytolytic cell activation against bovine herpes virus type 1-infected cells docs not require late viral glycoproteins. Immunology. 1989;66:565–569. [PMC free article] [PubMed] [Google Scholar]
  8. Edwards C. Changing trends in infectious bovine rhinotracheitis in Great Britain. Vet. Rec. 1988;123:614–618. [PubMed] [Google Scholar]
  9. Gibbs E.P.J., Rweyemamu M.M. Bovine herpesvirus-1. Vet. Bull. (London) 1977;47:317–343. [Google Scholar]
  10. Hutchings D.L., van Drunen Littel-van den Hurk S., Babiuk L.A. Lymphocyte proliferative responses to separated bovine herpesvirus 1 proteins in immune cattle. J. Virol. 1990;64:5114–5122. doi: 10.1128/jvi.64.10.5114-5122.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kelly D.H. Experimental infection of rabbits with the virus of infectious bovine rhinotracheitis. Br. J. Exp. Pathol. 1977;58:168–176. [PMC free article] [PubMed] [Google Scholar]
  12. Kit M., Kit S. Sensitive glycoprotein gIII blocking ELISA to distinguish between pseudorabes (Aujeszky's disease)-infectcd and vaceinated pigs. Vet. Microbiol. 1991;28:146–155. doi: 10.1016/0378-1135(91)90089-x. [DOI] [PubMed] [Google Scholar]
  13. Kit M., Kit S., Little S.P., Di Marchi R.D., Gale C. Bovine herpesvirus-1 (infectious bovine rhinotracheitis virus)-based viral vector which expresses foot-and-mouth disease epitopes. Vaccine. 1991;9:564–572. doi: 10.1016/0264-410x(91)90243-y. [DOI] [PubMed] [Google Scholar]
  14. Kit S. Genetically engineered pseudorabies and infectious bovine rhinotracheitis virus vaccines. In: Lasky L., editor. Vol. 84. Alan R. Liss; New York: 1988. pp. 183–195. (Technological Advances in Vaccine Development, UCLA Symposium on Molecular and Cellular Biology). [Google Scholar]
  15. Kit S. Rccombinant-derived modified-live herpesvirus vaccines. In: Atassi N.Z., editor. Immunology of Proteins and Peptides V. Plenum Publishing; New York: 1989. pp. 219–236. [Google Scholar]
  16. Kit S., Kit M. Genetically engineered herpesvirus vaccines. In: Melnick J.L., editor. Vol. 38. Karger; Basel: 1991. pp. 128–166. (Progress in Medical Virology.). [PubMed] [Google Scholar]
  17. Kit S., Qavi H., Gaines J.D., Billingsly P., McConnell S. Thymidine kinase-negative bovine herpesvirus type 1 mutant is stable and highly attenuated in calves. Arch. Virol. 1985;86:63–83. doi: 10.1007/BF01314114. [DOI] [PubMed] [Google Scholar]
  18. Kit S., Kit M., McConnell S. Intramuscular and intravaginal vaccination of pregnant cows with thymidine kinase-negative. temperature-resistant infectious bovine rhinotracheitis virus (bovine herpesvirus 1) Vaccine. 1986;4:55–61. doi: 10.1016/0264-410x(86)90098-8. [DOI] [PubMed] [Google Scholar]
  19. Kit S., Awaya Y., Otsuka H., Kit M. Blocking ELISA to distinguish pseudorabies virus-infected pigs from those vaccinated with a glycoprotein gIII deletion mutant. J. Vet. Diag. Invest. 1990;2:14–23. doi: 10.1177/104063879000200104. [DOI] [PubMed] [Google Scholar]
  20. Kit S., Otsuka H., Kit M. Gene-deleted IBRV marker vaccine. Vet. Rec. 1990;127:363–364. [PubMed] [Google Scholar]
  21. Liang X., Babiuk L.A., Zamb T.J. Pseudorabies virus gIII and bovine herpesvirus 1 gIII share complementary functions. J. Virol. 1991;65:5553–5557. doi: 10.1128/jvi.65.10.5553-5557.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ludwig G.V., Letchworth G.J., III Temporal control of bovine herpesvirus 1 glycoprotein synthesis. J. Virol. 1987;61:3292–3294. doi: 10.1128/jvi.61.10.3292-3294.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Meyer A.L., Petrovskis E.A., Duffus W.P.H., Thomsen D.R., Post L.E. Cloning and sequence of an infectious bovine rhinotracheitis virus (BHV-1) gene homologous to glycoprotein H of herpes simplex virus. Biochim. Biophys. Acta. 1991;1090:267–269. doi: 10.1016/0167-4781(91)90116-4. [DOI] [PubMed] [Google Scholar]
  24. Miller J.M., Whetstone C.A., Bello L.J., Lawrence W.C. Determination of ability of a thymidine kinase-negative deletion mutant of bovine herpesvirus-1 to cause abortion in cattle. Am. J. Vet. Res. 1991;52:1038–1943. [PubMed] [Google Scholar]
  25. Palmer L.D., Leary T.P., Wilson D.M., Splitter G.A. Bovine natural killer-like cell responses against cell lines expressing recombinant bovine herpesvirus type 1 glycoproteins. J. Immun. 1990;145:1009–1014. [PubMed] [Google Scholar]
  26. van Drunen Littel-van den Hurk S., Gifford G.A., Babiuk L.A. Epitope specificity of the protective immune response induced by individual bovine herpesvirus-1 glycoproteins. Vaccine. 1990;8:358–368. doi: 10.1016/0264-410x(90)90095-4. [DOI] [PubMed] [Google Scholar]

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