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. 2002 Nov 13;27(2):239–244. doi: 10.1016/0165-5728(90)90074-W

The effects of irradiation on major histocampatibility complex expression and lymphocytic infiltration in the normal rat brain and the 9L gliosarcoma brain tumor model

P Wen 1,∗, JS Loeffler 2, JH Morris 3, LA Lampson 1
PMCID: PMC7119745  PMID: 2332484

Abstract

The effects of irradiation on major histocompatibility complex (MHC) expression and lymphocytic infiltration in the normal rat brain and the 9L gliosarcoma brain tumor model were examined. Doses of irradiation administered were biologically equivalent to that used in the treatment of patients with malignant gliomas. No significant change in immune parameters was observed following irradiation in the normal rat brain. In the 9L gliosarcoma model irradiation did not suppress MHC expression or lymphocytic infiltration. These findings suggest that prior exposure to therapeutic irradiation need not adversely affect subsequent immunotherapies, and provide a foundation for future studies of immunomodulation in the irradiated brain.

Keywords: Irradiation, Major histocompatibility complex antigen, Lymphocytic infiltration, Brain tumor, 9L gliosarcoma

References

  1. Ellis F. Nominal standard dose and the rat. Br. J. Radiol. 1971;44:101–108. doi: 10.1259/0007-1285-44-518-101. [DOI] [PubMed] [Google Scholar]
  2. Ettinghausen S.E., Lipford E.H., Mule J.J., Rosenberg S.A. Systematic administration of recombinant interleukin 2 stimulates in vivo lymphoid proliferation in tissues. J. Immunol. 1985;135:1488–1497. [PubMed] [Google Scholar]
  3. Hellstrom K.E., Kant J.A., Tamerius J.D. Regression and inhibition of sarcoma growth by interference with a radiosensitive T-cell population. J. Exp. Med. 1978;148:799–804. doi: 10.1084/jem.148.3.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Henderson S.D., Kimler B.F., Morantz R.A. Radiation theraphy of 9L rat brain tumors. J. Radiat. Oncol. Biol. Phys. 1981;7:497–502. doi: 10.1016/0360-3016(81)90136-x. [DOI] [PubMed] [Google Scholar]
  5. Jager M.J., van der Pol J.P., de Wolff-Rouendaal D., de Jong P.T.V.M., Ruiter D.J. Decreased expression of HLA class II antigens on human uveal melanoma cells after in vivo X-ray irradiation. Am. J. Ophthalmol. 1988;105:78–86. doi: 10.1016/0002-9394(88)90125-0. [DOI] [PubMed] [Google Scholar]
  6. Kobayashi N., Allen N., Clendenon N.R., Ko L.-W. An improved rat brain tumor model. J. Neurosurg. 1980;53:808–815. doi: 10.3171/jns.1980.53.6.0808. [DOI] [PubMed] [Google Scholar]
  7. Kramer S., Henderson F., Zelen M., Schotz W. Therapeutic trials in the management of metastatic brain tumors by different time/dose fraction schemes of radiation therapy. Natl. Cancer Inst. Monogr. 1977;46:213–221. [PubMed] [Google Scholar]
  8. Lampson L.A. Molecular basis of the immune response to neural antigens. Trends Neurosci. 1987;10:211–216. [Google Scholar]
  9. Lampson L.A., Hickey W.F. Monoclonal antibody analysis of MHC expression in human brain biopsies: tissue ranging from ‘histologically normal’ to that showing different levels of glial tumor involvement. J. Immunol. 1986;136:4054–4062. [PubMed] [Google Scholar]
  10. Lampson L.A., Morris J.M., Roman V.A., Wen P., Sarid J. Interactions between leucocytes and individual brain tumor cells in the rat brain. J. Neurooncol. 1989 (abstract) (in press) [Google Scholar]
  11. Markoe A.M., Saluk P.H. Radiation immunobiology and immunomodulation. In: Perez C.A., Brady L.W., editors. Principles and Practice of Radiation Oncology. J.B. Lippincott; New York: 1987. [Google Scholar]
  12. McGeer P.L., Itagaki S., Boyes B.E., McGeer E.G. Reactive microglia are positive for HLA-DR in the substantia nigra of Parkinson's and Alzheimer's disease brains. Neurology. 1988;38:1285–1291. doi: 10.1212/wnl.38.8.1285. [DOI] [PubMed] [Google Scholar]
  13. North R.J. γ-Irradiation facilities the expression of adoptive immunity against established tumors by eliminating suppressor T cells. Cancer Immunol. Immunother. 1984;16:175–181. doi: 10.1007/BF00205425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schmidek H.H., Nielson S.L., Schillel A.L., Messer J. Morphological studies of rat brain tumors induced by N-nitrosomethylurea. J. Neurosurg. 1971;34:335–340. doi: 10.3171/jns.1971.34.3.0335. [DOI] [PubMed] [Google Scholar]
  15. Sobel R.A., Blanchette B.W., Atul K.B., Colvin R.B. The immunopathology of experimental allergic encephalomyelitis. II. Endothelial cell Ia increases prior to inflammatory infiltration. J. Immunol. 1984;132:2402–2407. [PubMed] [Google Scholar]
  16. Streit W.J., Graeber M.B., Kreutzberg G.W. Peripheral nerve lesion produces increased levels of MHC antigens in the CNS. J. Neuroimmunol. 1989;21:117–123. doi: 10.1016/0165-5728(89)90167-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Suzumura A., Lavi E., Weiss S.R., Silberberg D.H. Corona virus infection induces H-2 antigen expression in oligodendrocytes and astrocytes. Science. 1986;232:991–993. doi: 10.1126/science.3010460. [DOI] [PubMed] [Google Scholar]
  18. Tilkin A.F., Schaaf-Lafontaine N., Van Acker A. Vol. 78. 1981. Reduced tumor growth after low dose irradiation or immunization against blastic suppressor cells; pp. 1809–1812. (Proc. Natl. Acad. Sci. U.S.A.). [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Traugott U. Multiple sclerosis: relevance of class I and class II MHC expressing cells to lesion development. J. Neuroimmunol. 1987;16:283–302. doi: 10.1016/0165-5728(87)90082-8. [DOI] [PubMed] [Google Scholar]
  20. Whelan J.P., Chatten J., Lampson L.A. HLA class 1 and β2-microglobulin expression in frozen and formadehyde-fixed paraffin sections of neuroblastoma tumors. Cancer Res. 1985;45:5976–5983. [PubMed] [Google Scholar]
  21. Whelan J.P., Wysocki C.J., Lampson L.A. Distribution of β2-microglobulin in olfactory epithelium: a proliferating neuroepithelium not protected by a blood-tissue barrier. J. Immunol. 1986;137:2567–2571. [PubMed] [Google Scholar]
  22. Wong G.H., Bartlett P.F., Clark-Lewis I., McKimm-Breschkin J.L., Schrader J.W. Interferon-γ induces the expression of H-2 and Ia antigens on brain cells. J. Neuroimmunol. 1985;7:225–278. doi: 10.1016/s0165-5728(84)80026-0. [DOI] [PubMed] [Google Scholar]

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