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. 2002 Nov 12;14(3):229–235. doi: 10.1016/0166-0934(86)90024-8

An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy

AA Codd 1, HK Narang 1
PMCID: PMC7119784  PMID: 3025238

Abstract

Faecal specimens from 520 patients with non-bacterial, gastroenteritis were examined by electron microscopy using four methods. These were (1) a direct dip method, (2) low-speed centrifugation, (3) ultracentrifugation and (4) a calcium phosphate method. The calcium phosphate method combined with low-speed centrifugation (750 × g, 2,100 × g) was considered overall best. The calcium phosphate method makes it possible to handle a large number of faecal specimens by saving considerable time and labour.

Keywords: faecal specimen, gastroenteritis, adenovirus, rotavirus, small round virus, coronavirus

References

  1. Caul E.O., Ashley C.R., Egglestone S.I. An improved method for the routine identification of faecal viruses using ammonium sulphate precipitation. FEMS Microbiol. Lett. 1978;4:1–4. [Google Scholar]
  2. Flewett T.H., Bryden S.A., Davies H.A. Virus particles in gastroenteritis. Lancet. 1973;ii:1497. doi: 10.1016/s0140-6736(73)92760-8. [DOI] [PubMed] [Google Scholar]
  3. Flewett T.H., Bryden S.A., Davies H., Woode G.N., Bridger J.C., Derrick J.M. Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet. 1974;ii:61–63. doi: 10.1016/s0140-6736(74)91631-6. [DOI] [PubMed] [Google Scholar]
  4. Madeley C.R., Cosgrove B.P., Bell E.J., Fallon R.J. Stool viruses in babies in Glasgow. 1. Hospital admissions with diarrhoea. J. Hygiene. 1977;78:261–273. doi: 10.1017/s0022172400056151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mathan M., Mathan V.I., Swaninathan S.P., Yesudos S., Baker S.J. Pleomorphic viruslike particle in human faeces. Lancet. 1975;i:1192. doi: 10.1016/S0140-6736(75)91832-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Middleton P.J., Szymanski M.T., Abbott G.D., Bortolussi R., Hamilton J.R. Orbivirus acute gastroenteritis of infancy. Lancet. 1974;i:1241–1244. [Google Scholar]
  7. Narang H.K., Codd A.A. Low-speed centrifugation technique for the preparation of grids for direct virus examination by electron microscopy. J. Clin. Pathol. 1979;32:304–305. doi: 10.1136/jcp.32.3.304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Narang H.K., Codd A.A. Preponderance of rotavirus in clumped form in patients with acute gastroenteritis. Lancet. 1980;i:1192–1193. doi: 10.1016/s0140-6736(80)91650-5. [DOI] [PubMed] [Google Scholar]
  9. Narang H.K., Codd A.A. Enterovirus typing by immune electron microscopy using lowspeed centrifugation. J. Clin. Pathol. 1980;33:191–194. doi: 10.1136/jcp.33.2.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stain E.L., Rhodes M.B., White R.G., Mebus C.A. Neonatal calf diarrhoea: purification and electron microscopy of corona-like agent. Am. J. Vet. Res. 1972;33:1147–1156. [PubMed] [Google Scholar]
  11. Taverne J., Wildy P. Purification of herpes simplex virus by chromatography of calcium phosphate. Nature. 1959;184:1655–1656. doi: 10.1038/1841655a0. [DOI] [PubMed] [Google Scholar]
  12. Taverne J., Marshall J.H., Fulton F. The purification and concentration of viruses and virus soluble antigens on calcium phosphate. J. Gen. Microbiol. 1958;19:451–461. doi: 10.1099/00221287-19-3-451. [DOI] [PubMed] [Google Scholar]
  13. Tiselius A., Hjerten S., Levin O. Protein chromatography on calcium phosphate columns. Arch. Biochem. Biophys. 1956;65:132–155. doi: 10.1016/0003-9861(56)90183-7. [DOI] [PubMed] [Google Scholar]

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