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. 2002 Dec 10;50(1):237–244. doi: 10.1016/0166-0934(94)90180-5

Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6

Claude Daniel 1, Alain Lamarre 1, Pierre J Talbot 1,∗
PMCID: PMC7119785  PMID: 7714047

Abstract

Infection of cell monolayers by murine coronavirus A59 at pH 6 rather than 7 yielded a ten-fold increase in the infectious titer and a remarkable enhancement of the reactivities of monoclonal and polyclonal antibodies against the spike glycoprotein in immunoblotting, immuno-precipitation and enzyme-linked immunosorbent assays. These observations are very useful for detecting antibodies against the S glycoprotein of coronaviruses and enhancing infectious titers.

Keywords: Coronavirus, Infectivity, S glycoprotein, Antibody, Antigenicity

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