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. 2002 Nov 13;32(1):1–9. doi: 10.1016/0165-5728(91)90065-F

Production of mice from a lethal coronavirus infection in the central nervous system by adoptive transfer of virus-specific T cell clones

Kenjiro Yamaguchi 1,∗, Naoaki Goto 2, Shigeru Kyuwa 1,†, Masanori Hayami 1,∗∗, Yutaka Toyoda 1
PMCID: PMC7119813  PMID: 1705943

Abstract

The protective effect of a mouse hepatitis virus type-4 (MHV-4)-specific CD8+ cytotoxic T cell clone and a CD4+ helper T cell clone was examined by the adoptive transfer into brains of mice lethally infected with MHV-4. Mice survived acute encephalitis if more than 5 × 105 cells of either type of the virus-specific T cell clones had been transfered into H-2-matched recipients by 1 day post-infection. Although the adoptive transfer of both types of the T cell clones suppressed viral growth and viral antigen-positive cells in the brains, a significant inhibition of virus replication by the cytotoxic T cell clone was detected prior to that induced by the helper T cell clone. Histologically, cell destruction was prominent in the brains of mice which received the cytotoxic T cell clone. These results demonstrate that both the CD8+ cytotoxic T cell and the CD4+ helper T cell can protect mice from a lethal MHV-4 infection in the central nervous system.

Keywords: CD4+ helper T cell, CD8+ cytotoxic T cell, Cellular immunity, Central nervous system, Viral infection

References

  1. Blanden R.V., Gardner I.D. The cell-mediated immune response to ectromelia virus infection. I. Kinetics and characteristics of the primary effector T cell response in vivo. Cell. Immunol. 1976;22:271–282. doi: 10.1016/0008-8749(76)90029-0. [DOI] [PubMed] [Google Scholar]
  2. Bryne J.A., Oldstone M.B.A. Biology of cytotoxic T lymphocytes specific for lymphocytic choriomeningitis virus: clearance of virus in vivo. J. Virol. 1984;51:682–686. doi: 10.1128/jvi.51.3.682-686.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Buchmeier M.J., Lewicki H.A., Talbot P.J., Knobler R.L. Murine hepatitis virus (strain JHM)-induced neurologic disease is modulated in vivo by monoclonal antibody. Virology. 1984;132:261–270. doi: 10.1016/0042-6822(84)90033-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fleming J.O., Shubin R.A., Sussman M.A., Casteel N., Stohlman S.A. Monoclonal antibodies to the matrix (E1) glycoprotein of mouse hepatitis virus protect mice from encephalitis. Virology. 1989;168:162–167. doi: 10.1016/0042-6822(89)90415-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hirano N., Murakami T., Fujiwara K., Matsumoto M. Utility of mouse cell line DBT for propagation and assay of mouse hepatitis virus. Jpn. J. Exp. Med. 1978;48:71–74. [PubMed] [Google Scholar]
  6. Kaplow L.S. Substitute for benzidine in myeloperoxidase stains. Am. J. Clin. Pathol. 1975;63:451. doi: 10.1093/ajcp/63.3.451. [DOI] [PubMed] [Google Scholar]
  7. Knobler R.L., Dubois-Dalcq M., Haspel M.V., Claysmith A.P., Lampert P.W., Oldstone M.B.A. Selective localization of wild type and mutant mouse hepatitis virus (JHM strain) antigens in CNS tissue by fluorescence, light and electron microscopy. J. Neuroimmunol. 1981;1:81–92. doi: 10.1016/0165-5728(81)90010-2. [DOI] [PubMed] [Google Scholar]
  8. Knobler R.L., Haspel M.V., Oldstone M.B.A. Mouse hepatitis virus type 4 (JHM strain)-induced fatal central nervous system disease. I. Genetic control and the murine neuron as the susceptible site of disease. J. Exp. Med. 1981;153:832–843. doi: 10.1084/jem.153.4.832. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Knobler R.L., Tunison L.A., Lampert P.W., Oldstone M.B.A. Selected mutants of mouse hepatitis virus type 4 (JHM strain) induce different CNS disease. Am. J. Pathol. 1982;109:157–168. [PMC free article] [PubMed] [Google Scholar]
  10. Kyuwa S., Stohlman S.A. Pathogenesis of a murine coronavirus, strain JHM infection in the central nervous system of mice. Semin. Virol. 1990;1:273–280. [Google Scholar]
  11. Kyuwa S., Yamaguchi K., Hayami M., Fujiwara K. Characterization of mouse hepatitis virus-reactive T cell clones. Adv. Exp. Med. Biol. 1987;218:391–398. doi: 10.1007/978-1-4684-1280-2_48. [DOI] [PubMed] [Google Scholar]
  12. Lavi E., Suzumura A., Murasko D.K., Murray E.M., Silberberg D.H., Weiss S.R. Tumor necrosis factor induces expression of MHC class I antigens on mouse astrocytes. J. Neuroimmunol. 1988;18:245–253. doi: 10.1016/0165-5728(88)90102-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lukacher A.E., Braciale V.L., Braciale T.J. In vivo effector function of influenza virus-specific cytotoxic T lymphocyte clones is highly specific. J. Exp. Med. 1984;160:814–826. doi: 10.1084/jem.160.3.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mullbacher A., Ada G.L. How do cytotoxic T lymphocytes work in vivo? Microb. Pathogen. 1987;3:315–318. doi: 10.1016/0882-4010(87)90001-5. [DOI] [PubMed] [Google Scholar]
  15. Oldstone M.B.A., blount P., Southern P.J., Lampert P.W. Cytoimmunotherapy for persistent virus infection reveals a unique clearance pattern from the central nervous system. Nature. 1986;321:239–243. doi: 10.1038/321239a0. [DOI] [PubMed] [Google Scholar]
  16. Stohlman S.A., Frelinger J.A. Resistance to fatal central nervous system disease by mouse hepatitis virus, strain JHM. I. Genetic analysis. Immunogenetics. 1978;6:277–281. [Google Scholar]
  17. Stohlman S.A., Matsushima G.K., Casteel N., Weiner L.P. In vivo effect of coronavirus-specific T cell clones: DTH inducer cells prevent a lethal infection but do not inhibit virus replication. J. Immunol. 1986;136:3052–3056. [PubMed] [Google Scholar]
  18. Sussman M.A., Shubin R.A., Kyuwa S., Stohlman S.A. T-cell-mediated clearance of JHM virus from the central nervous system. J. Virol. 1989;63:3051–3056. doi: 10.1128/jvi.63.7.3051-3056.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Suzumura A., Levi E., Weiss S.R., Silberberg D.H. Coronavirus infection induces H-2 antigen expression on oligodendrocytes and astrocytes. Science. 1986;232:991–993. doi: 10.1126/science.3010460. [DOI] [PubMed] [Google Scholar]
  20. Sweetser M.T., Morrison L.A., Braciale V.L., Braciale T.J. Recognition of pre-processed endogenous antigen by class I but not class II MHC-restricted T cells. Nature. 1989;342:180–182. doi: 10.1038/342180a0. [DOI] [PubMed] [Google Scholar]
  21. Townsend A., Bodmer H. Antigen recognition by class I-restricted T lymphocytes. Annu. Rev. Immunol. 1989;7:601–624. doi: 10.1146/annurev.iy.07.040189.003125. [DOI] [PubMed] [Google Scholar]
  22. Vitetta E.S., Capra J.D. The protein products of the murine 17th chromosome: genetics and structure. Adv. Immunol. 1978;26:147–193. doi: 10.1016/s0065-2776(08)60230-8. [DOI] [PubMed] [Google Scholar]
  23. Wege H., Siddell S., ter Meulen V. The biology and pathogenesis of coronavirus. Curr. Top. Microbiol. Immunol. 1982;99:165–200. doi: 10.1007/978-3-642-68528-6_5. [DOI] [PubMed] [Google Scholar]
  24. Williams K.A., Hart D.N.J., Fabre J.W., Morris P.J. Distribution and quantitation of HLA-A, B, C and DR (Ia) antigens on human kidney and other tissues. Transplantation. 1980;29:274–279. doi: 10.1097/00007890-198004000-00002. [DOI] [PubMed] [Google Scholar]
  25. Williamson J.S.P., Stohlman S.A. Effective clearance of mouse hepatitis virus from the central nervous system requires both CD 4+ and CD 8+ T cells. J. Virol. 1990;9:4589–4592. doi: 10.1128/jvi.64.9.4589-4592.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Witton J.L., Oldstone M.B.A. Class I MHC can present an endogenous peptide to cytotoxic T lymphocytes. J. Exp. Med. 1989;170:1033–1038. doi: 10.1084/jem.170.3.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wong G.H.W., Bartlett P.F., Clark-Lewis I., Battye F., Schrader J.W. Inducible expression of H-2 and Ia antigens on brain cells. Nature. 1984;310:688–691. doi: 10.1038/310688a0. [DOI] [PubMed] [Google Scholar]
  28. Wong G.H.W., Bartlett P.F., Clark-Lewis I., McKimm-Breschkin J.L., Schrader J.W. Interferon-γ induces the expression of H-2 and Ia antigens on brain cells. J. Neuroimmunol. 1985;7:255–278. doi: 10.1016/s0165-5728(84)80026-0. [DOI] [PubMed] [Google Scholar]
  29. Yamaguchi K., Kyuwa S., Nakanaga K., Hayami M. Establishment of cytotoxic T-cell clones specific for cells infected with mouse hepatitis virus. J. Virol. 1988;62:2505–2507. doi: 10.1128/jvi.62.7.2505-2507.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yamamoto S., Iwai H., Ueda K. Establishment of tuberculous antigen-specific T cell line and its effect on hepatic granuloma formation in BCG-infected nude mice. Jpn. J. Vet. Sci. 1988;50:215–225. doi: 10.1292/jvms1939.50.215. [DOI] [PubMed] [Google Scholar]
  31. Yap K.L., Ada G.L., McKenzie I.F.C. Transfer of specific cytotoxic lymphocytes protects mice inoculated with influenza virus. Nature. 1978;273:238–239. doi: 10.1038/273238a0. [DOI] [PubMed] [Google Scholar]

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