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. 2002 Nov 13;19(1):159–165. doi: 10.1016/0165-5728(88)90045-8

Cytotoxic effect of myelin basic protein-reactive T cells on cultured oligodendrocytes

Kuniyuki Kawai 1,∗, Burton Zweiman 2
PMCID: PMC7119855  PMID: 2456302

Abstract

To help clarify effector mechanisms in experimental allergic encephalitis (EAE), the cytotoxic effects of myelin basic protein (MBP)-reactive lymphocytes on oligodendrocytes were studied using a 51Cr release assay. MBP-reactive encephalitogenic T cell lines were cytotoxic to 51Cr-labeled oligodendrocyte target cells derived from Lewis rat fetal brain-dissociated culture, when incubated for 6 h in the presence of antigen-presenting cells (APC) and MBP (percent51Cr release = 65±3% vs. spontaneous release = 22±3% vs. normal lymph node cells + APC and MBP = 20 ± 3%). This reaction is time dependent, likely MHC restricted, and is not just a nonspecific toxic effect against any Lewis target cells since neither fibroblasts nor astrocytes were affected. Other (tetanus toxoid-reactive) lymphoblasts stimulated by specific antigen were not cytotoxic to the oligodendrocytes. These findings suggest that oligodendrocytes might be target cells for MBP-reactive lymphocytes in EAE if antigen presentation is appropriate.

Keywords: Experimental allergic encephalitis, Oligodendrocyte, Myelin basic protein-reactive lymphocyte, Cytotoxic test, 51Cr release assay

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