Skip to main content
NCBI home page
Elsevier - PMC COVID-19 Collection logoLink to Elsevier - PMC COVID-19 Collection
. 2002 Nov 11;22(2):107–111. doi: 10.1016/0165-5728(89)90040-4

Induciton of MHC class I antigens on glial cells is dependent on persistent mouse hepatitis virus infection

Ehud Lavi 1,∗, Akio Suzumura 2,∗∗, Edward M Murray 1, Donald H Silberberg 2, Susan R Weiss 1,∗∗∗
PMCID: PMC7119878  PMID: 2538490

Abstract

H-2 class I antigens, but not class II antigens, were detected on the surface of glial cells persistently infected with mouse hepatitis virus strain A59 (MHV-A59) as late as 90 days post-infection. Uninfected glial cells remained negative for H-2 class I and class II surface antigens. We have previously shown that conditioned media from infected glial cell cultures (supernatants) contain a factor unrelated to infectious virus and capable of inducing H-2 class I antigens on uninfected glial cells. The synthesis of this factor appears to be dependent on production of infectious virus since the H-2 inducing activity could not be detected 3 days following the addition of neutralizing antibodies to the cultures. This suggests that H-2 inducing activity contains as unstable component, the synthesis of which is dependent on continual virus production. Persistent MHV infection and H-2 class I antigen expression may play a role in MHV-induced demyelination.

Keywords: Major histocompatibility complex class I induction; Mouse hepatitis virus, persistent infection; Glial cell; Demyelination, virus-induced

References

  1. Fishman P.S., Gass J.S., Swoveland P.T., Lavi E., Highkin M.K., Weiss S.R. Infection of the basal ganglia by a murine coronavirus. Science. 1985;229:877–879. doi: 10.1126/science.2992088. [DOI] [PubMed] [Google Scholar]
  2. Knobler R.L., Lampert P.W., Oldstone M.B.A. Virus persistence and recurring demyelination produced by a temperature sensitive mutant of MHV-4. Nature. 1982;298:279–280. doi: 10.1038/298279a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Lampert P.W., Sims S.K., Kniazeff A.J. Mechanism of demyelination in JHM virus induced encephalomyelitis, electron microscopic studies. Acta Neuropathol. 1973;24:76–85. doi: 10.1007/BF00691421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lavi E., Gilden D.H., Highkin M.K., Weiss S.R. Persistence of mouse hepatitis virus A59 in a slow virus demyelinating infection in mice as detected by in situ hybridization. J. Virol. 1984;51:563–566. doi: 10.1128/jvi.51.2.563-566.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lavi E., Gilden D.H., Wroblewska Z., Rorke L.B., Weiss S.R. Experimental demyelination produced by the A59 strain of mouse hepatitis virus. Neurology. 1984;4:597–603. doi: 10.1212/wnl.34.5.597. [DOI] [PubMed] [Google Scholar]
  6. Lavi E., Gilden D.H., Highkin M.K., Weiss S.R. The organ tropism of mouse hepatitis virus A59 is dependent on dose of route of inoculation. Lab. Anim. Sci. 1986;36:130–135. [PubMed] [Google Scholar]
  7. Lavi E., Suzumura A., Hirayama M., Highkin M.K., Dambach D.M., Silberberg D.H., Weiss S.R. Coronavirus MHV-A59 causes a persistent, productive infection in glial cells. Microb. Pathog. 1987;3:79–86. doi: 10.1016/0882-4010(87)90066-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lavi E., Fishman P.S., Highkin M.K., Weiss S.R. Limbic encephalitis following inhalation of murine coronavirus MHV-A59. Lab. Invest. 1988;56:31–36. [PubMed] [Google Scholar]
  9. McCarthy K.D., de Vellis J. Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J. Cell Biol. 1980;85:890–902. doi: 10.1083/jcb.85.3.890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stohlman S.A., Weiner L.P. Chronic central nervous system demyelination in mice after JHM virus infection. Neurology. 1981;31:38–44. doi: 10.1212/wnl.31.1.38. [DOI] [PubMed] [Google Scholar]
  11. Suzumura A., Silberberg D.H. Expressiion of H-2 antigen on oligodendrocytes is induced by soluble factors from concanavalin A activated T cells. Brain Res. 1985;336:171–175. doi: 10.1016/0006-8993(85)90431-7. [DOI] [PubMed] [Google Scholar]
  12. Suzumura A., Bhat S., Ecclestone A., Lisak R.P., Silberberg D.H. The isolation and long-term culture of oligodendrocytes from newborn mouse brain. Brain Res. 1984;324:379–383. doi: 10.1016/0006-8993(84)90054-4. [DOI] [PubMed] [Google Scholar]
  13. Suzumura A., Lavi E., Weiss S.R., Silberberg D.H. Coronavirus infection induces H-2 antigen expression on oligodendrocytes and astrocytes. Science. 1986;232:991–993. doi: 10.1126/science.3010460. [DOI] [PubMed] [Google Scholar]
  14. Suzumura A., Lavi E., Bhat S., Weiss S.R., Silberberg D.H. Induction of glial cell MHC antigen expression in neurotropic coronavirus infection: characterization of the H-2 induces soluble factor elaborated by infected brain cells. J. Immunol. 1988;140:2068–2072. [PubMed] [Google Scholar]
  15. Watanabe R., Wege H., ter Meulen V. Adoptive transfer of EAE-like lesions from rats with coronavirus induced demyelinating encephalomyelites. Nature. 1983;305:150–153. doi: 10.1038/305150a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wong G.H.W., Bartlett R.F., Clar-Lewis I., Battye F., Shrader J.W. Inducible expression of H-2 and 1a antigens on brain cells. Nature. 1984;310:688–691. doi: 10.1038/310688a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Neuroimmunology are provided here courtesy of Elsevier

RESOURCES