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. 2002 Nov 13;43(3):203–208. doi: 10.1016/0165-2478(94)90224-0

Ontogeny of interferon alpha secreting cells in the porcine fetal hematopoietic organs

Igor Šplíchal c, Michel Bonnneau b, Bernard Charley a,∗
PMCID: PMC7119891  PMID: 7721334

Abstract

We examined the ontogeny of IFN-α Secreting Cells (IFN-α SC) in different hematopoietic organs and blood of porcine fetuses at different stages of gestation. Cells were induced to produce IFN-α by incubation with the coronavirus TGEV and IFN-α SC were detected by ELISPOT. A striking finding was that IFN-α SC could be detected in the fetal liver as early as at 26 days of gestation, i.e., during the first quarter of gestation, a period at which T-cell markers could not be detected by flow cytometry. In addition, IFN-α SC could be detected in the cord blood, the spleen and the bone marrow of fetuses at later stages of gestation. These data indicate that IFN-α SC appear very early during the ontogeny of the immune system, long before the development of the specific immune system, and may therefore represent an early antiviral defence mechanism. IFN-α SC were found to be associated with hematopoietic organs, which argues for their hematopoietic lineage.

Keywords: Interferon, Coronavirus, Ontogeny, Leukocytes, Porcine, ELISPOT

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