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. 2009 Dec 30:533–580. doi: 10.1007/978-1-59745-405-6_21

Molecular Virology

Josephine Wu 4,5, Mona Sharaan 4,5, David Y Zhang 4,5
Editors: Liang Cheng1, David Y Zhang2,3
PMCID: PMC7121188

Abstract

Traditional viral isolation by cell culture assays and conventional serological methods have been previously used to detect and identify various virus infections

Keywords: Human Immunodeficiency Virus, Herpes Simplex Virus, Human Papilloma Virus, Avian Influenza, Severe Acute Respiratory Syndrome

Suggested reading

  1. Alejo E. Resistance of human cytomegalovirus to antiviral drugs. Clin Microbiol Rev. 1999;12:286–297.. doi: 10.1128/cmr.12.2.286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ando Y., Terao K., Narita M., et al. Quantitative analyses of cytomegalovirus genome in aqueous humor of patients with cytomegalovirus retinitis. Jpn. J. Ophthalmol. 2002;46:254–260.. doi: 10.1016/S0021-5155(01)00524-X. [DOI] [PubMed] [Google Scholar]
  3. Anzola M., Burgos J.J. Hepatocellular carcinoma: molecular interactions between hepatitis C virus and p53 in hepatocarcinogenesis. Expert Rev Mol Med. 2003;5:19.. doi: 10.1017/S1462399403006926. [DOI] [PubMed] [Google Scholar]
  4. Boeckh M., Boivin G. Quantitation of cytomegalovirus: methodologic aspects and clincial applications. Clin Microbiol Rev. 1998;11:533–554.. doi: 10.1128/cmr.11.3.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bowen E.F., Sabin C.A., Wilson P., et al. Cytomegalovirus (CMV) viremia detected by polymerase chain reaction identifies a group of HIV-positive patients at high risk of CMV disease. AIDS. 1997;11:889–893.. doi: 10.1097/00002030-199707000-00008. [DOI] [PubMed] [Google Scholar]
  6. Cope A.V., Sabin C., Burroughs A., et al. Interrelationships among quantity of human cytomegalovirus (HCMV) DNA in blood, donor-recipient serostatus, and administration of methylprednisolone as risk factors for HCMV disease following liver transplantation. J. Infect. Dis. 1997;176:1484–1490.. doi: 10.1086/514145. [DOI] [PubMed] [Google Scholar]
  7. Cockerill F.R., Smith T.F. Response of the clinical microbiology laboratory to emerging (new) and reemerging infectious diseases. J. Clin Microbiol. 2004;42:2359–2365.. doi: 10.1128/JCM.42.6.2359-2365.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Comanor L., Hendricks D. Hepatitis C virus RNA tests: performance attributes and their impact on clinical utility. Expert Rev Mol Diagn. 2003;3:689–701.. doi: 10.1586/14737159.3.6.689. [DOI] [PubMed] [Google Scholar]
  9. Cope A.V., Sweny P., Sabin C., et al. Quantity of cytomegalovirus viruria is a major risk factor for cytomegalovirus disease after renal transplantation. J. Med. Virol. 1997;52:200–205.. doi: 10.1002/(SICI)1096-9071(199706)52:2<200::AID-JMV14>3.0.CO;2-O. [DOI] [PubMed] [Google Scholar]
  10. Ellermann-Eriksen S. Macrophages and cytokines in the early defense against herpes simplex virus. Virol J. 2005;2:1–30.. doi: 10.1186/1743-422X-2-59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Espy M., Uhl J.R., Sloan L.M., et al. Real-Time PCR in clinical microbiology: applications for routine laboratory testing. Clin Microbiol Rev. 2006;19:165–256.. doi: 10.1128/CMR.19.1.165-256.2006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gault E., Michel Y., Dehée A., Belabani C., et al. Quantification of human cytomegalovirus DNA by real-time PCR. J. Clin. Microbiol. 2001;39:772–775.. doi: 10.1128/JCM.39.2.772-775.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gor D., Sabin C., Prentice H.G., et al. Longitudinal fluctuation in cytomegalovirus load in bone marrow transplant patients: relationship between peak virus load, donor/recipient serostatus, acute GVHD and CMV disease. Bone Marrow Transplant. 1998;21:597–605.. doi: 10.1038/sj.bmt.1701139. [DOI] [PubMed] [Google Scholar]
  14. Kearns A.M., Guiver M., James V., et al. Development and evaluation of a real-time quantitative PCR for the detection of human cytomegalovirus. J. Virol. Methods. 2001;95:121–131.. doi: 10.1016/S0166-0934(01)00307-X. [DOI] [PubMed] [Google Scholar]
  15. Kearns A.M., Turner A.J., Eltringham G.J., et al. Rapid detection and quantification of CMV DNA in urine using LightCycler-based real-time PCR. J. Clin. Virol. 2002;24:131–134.. doi: 10.1016/S1386-6532(01)00240-2. [DOI] [PubMed] [Google Scholar]
  16. Kühn J.E., Wendland T., Schäfer P., et al. Monitoring of renal allograft recipients by quantitation of human cytomegalovirus genomes in peripheral blood leukocytes. J. Med. Virol. 1994;44:398–405.. doi: 10.1002/jmv.1890440416. [DOI] [PubMed] [Google Scholar]
  17. Landry M.L., Garner R., Ferguson D. Comparison of the NucliSens basic kit (nucleic acid sequence-based amplification) and the Argene Biosoft enterovirus consensus reverse transcription-PCR assays for rapid detection of enterovirus RNA in clinical specimens. J Clin Microbiol. 2003;41:5006–5010.. doi: 10.1128/JCM.41.11.5006-5010.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Landry M.L., Garner R., Ferguson D. Real-Time nucleic acid sequence-based amplification using molecular beacons for detection of enterovirus RNA in clinical specimens. J Clin Microbiol. 2005;43:3136–3139.. doi: 10.1128/JCM.43.7.3136-3139.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Machida U., Kami M., Fukui T., et al. Real-time automated PCR for early diagnosis and monitoring of cytomegalovirus infection after bone marrow transplantation. J. Clin. Microbiol. 2000;38:2536–2542.. doi: 10.1128/jcm.38.7.2536-2542.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mahony J.B., Richardson S. Molecular diagnosis of severe acute respiratory syndrome. J Molec Diagn. 2005;7:551–559.. doi: 10.1016/S1525-1578(10)60587-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Moore C., Valappil M., Corden S., et al. Enhanced clinical utility of the NucliSens EasyQ RSV A+B Assay for rapid detection of respiratory syntitial virus in clinical samples. Eur J Clin Microbiol Infect Dis. 2006;25:167–174.. doi: 10.1007/S10096-006-0112-4. [DOI] [PubMed] [Google Scholar]
  22. Moret H., Brodard V., Barranger C., et al. New commercially available PCR and microplate hybridization assay for detection and differentiation of human polyomaviruses JC and BK in cerebrospinal fluid, serum, and urine samples. J Clin Microbiol. 2006;44:1305–1309.. doi: 10.1128/JCM.44.4.1305-1309.2006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Murray P.G., Young L.S. Epstein-Barr virus infection: basis of malignancy and potential for therapy. Expert Rev Molec Med. 2001;3:1–20.. doi: 10.1017/S1462399401002575. [DOI] [PubMed] [Google Scholar]
  24. Nitsche A., Steuer N., Schmidt C.A., et al. Detection of human cytomegalovirus DNA by real-time quantitative PCR. J. Clin. Microbiol. 2000;38:2734–2737.. doi: 10.1128/jcm.38.7.2734-2737.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Neville B.W., Dam D.D., Allen C.M., Bouquot J.E. Oral and Maxillofacial Pathology. Philadelphia, PA: WB Saunders Company; 1995. [Google Scholar]
  26. Oberste M.S., Pallansch M.A. Enterovirus molecular detection and typing. Rev Med Microbiol. 2005;16:163–171.. [Google Scholar]
  27. Podzorski R.P. Molecular testing in the diagnosis and management of hepatitis C virus infection. Arch Path Lab Med. 2002;126:285–290.. doi: 10.5858/2002-126-0285-MTITDA. [DOI] [PubMed] [Google Scholar]
  28. Rasmussen L., Zipeto D., Wolitz R.A., et al. Risk for retinitis in patients with AIDS can be assessed by quantitation of threshold levels of cytomegalovirus DNA burden in blood. J. Infect. Dis. 1997;176:1146–1155.. doi: 10.1086/514106. [DOI] [PubMed] [Google Scholar]
  29. Russell W.C. Update on adenovirus and its vectors. J Gen Virol. 2000;81:2573–2604.. doi: 10.1099/0022-1317-81-11-2573. [DOI] [PubMed] [Google Scholar]
  30. Shinkai M., Bozzette S.A., Powderly W., et al. Utility of urine and leukocyte cultures and plasma DNA polymerase chain reaction for identification of AIDS patients at risk for developing human cytomegalovirus disease. J. Infect. Dis. 1997;175:302–308.. doi: 10.1093/infdis/175.2.302. [DOI] [PubMed] [Google Scholar]
  31. Spector S.A., Wong R., Hsia K., et al. Plasma cytomegalovirus (CMV) DNA load predicts CMV disease and survival in AIDS patients. J. Clin. Invest. 1998;101:497–502.. doi: 10.1172/JCI1101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Taubenberger J.K., Layne S.P. Diagnosis of influenza virus: coming to grips with the molecular era. Mol Diagn. 2001;6:291–305.. doi: 10.1054/modi.2001.28063. [DOI] [PubMed] [Google Scholar]
  33. Tanaka N., Kimura H., Iida K., et al. Quantitative analysis of cytomegalovirus load using a real-time PCR assay. J. Med. Virol. 2000;60:455–462.. doi: 10.1002/(SICI)1096-9071(200004)60:4<455::AID-JMV14>3.0.CO;2-Q. [DOI] [PubMed] [Google Scholar]
  34. Toyoda M., Carlos J.B., Galera O.A., et al. Correlation of cytomegalovirus DNA levels with response to antiviral therapy in cardiac and renal allograft recipients. Transplantation. 1997;63:957–963.. doi: 10.1097/00007890-199704150-00009. [DOI] [PubMed] [Google Scholar]
  35. Whiley D.M., Mackay I.M., Sloots T.P. Detection and differentiation of human polyomaviruses JC and BK by LightCycler PCR. J Clin Microbiol. 2001;39:4357–4361.. doi: 10.1128/JCM.39.12.4357-4361.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Zaia J.A., Gallez-Hawkins G.M., Tegtmeier B.R., et al. Late cytomegalovirus disease in marrow transplantation is predicted by virus load in plasma. J. Infect. Dis. 1997;176:782–785.. doi: 10.1086/517301. [DOI] [PubMed] [Google Scholar]
  37. Zoulim F. New nucleic acid diagnostic tests in viral hepatitis. Semin Liver Dis. 2006;26:309–317.. doi: 10.1055/s-2006-951602. [DOI] [PubMed] [Google Scholar]

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