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. 2007:213–222. doi: 10.1007/978-3-540-37885-3_25

Use of IgY Antibodies in Human and Veterinary Medicine

Rudiger Schade 6, Xiao-Ying Zhang 7, Horacio Raúl Terzolo 8
Editors: Rainer Huopalahti1, Rosina López-Fandiño2, Marc Anton3, Rüdiger Schade4
PMCID: PMC7121454

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Contributor Information

Rainer Huopalahti, Email: rainer.huopalahti@utu.fi.

Rosina López-Fandiño, Email: rosina@ifi.csic.es.

Marc Anton, Email: anton@nantes.inra.fr.

Rüdiger Schade, Email: ruediger.schade@charite.de.

Rudiger Schade, Email: ruediger.schade@charite.de.

Xiao-Ying Zhang, Email: zhxying@yahoo.com.

Horacio Raúl Terzolo, Email: hterzolo@balcarce.inta.gov.ar.

References

  1. Ahmed N, Rice GE. Strategies for revealing lower abundance proteins in two-dimensional protein maps. J Chromatogr B. 2005;815:39–50. doi: 10.1016/j.jchromb.2004.10.070. [DOI] [PubMed] [Google Scholar]
  2. Almeida CM, Kanashiro MM, Rangel Filho FB, Mata MF, Kipnis TL, da Silva WD. Development of snake antivenom antibodies in chickens and their purification from yolk. Vet Record. 1998;143:579–584. doi: 10.1136/vr.143.21.579. [DOI] [PubMed] [Google Scholar]
  3. Arasteh N, Aminirissehei A-H, Yousif AN, Albright LJ, Durance TD. Passive immunization of rainbow trout (Oncorhynchus mykiss) with chicken egg yolk immunoglobulins (IgY) Aquaculture. 2004;231:23–36. doi: 10.1016/j.aquaculture.2003.11.004. [DOI] [Google Scholar]
  4. Bilbao GN, Chacana PA, Mendiburu A, Rodríguez E, Blackhall JO, Terzolo HR. Profilaxis de la diarrea neonatal de terneros de tambo mediante suplementación con inmunoglobulinas de yema de huevo (IgY) Rev Med Vet (Bs As) 2006;84:135–139. [Google Scholar]
  5. Calzado EG, Marino EC, Chavez TS, Vazquez EL, Ochoa ZC, Schade R. Extraction of a monospecific Coombs-reagent from chicken eggs. ALTEX. 2003;20:21–25. [PubMed] [Google Scholar]
  6. Carlander D. Avian IgY antibody, in vitro and in vivo. Acta Universitatis Upsaliensis, Comprehensive summaries of uppsala dissertations from the faculty of medicine 1119. Sweden: University of Uppsala; 2002. pp. 1–153. [Google Scholar]
  7. Carlander D, Stalberg J, Larsson A. Chicken antibodies. A clinical chemistry perspective. Upps J Med Sc. 1999;104:179–190. doi: 10.3109/03009739909178961. [DOI] [PubMed] [Google Scholar]
  8. Carlander D, Kollberg H, Wejaker P-E, Larsson A. Peroral immunotherapy with yolk antibodies for the prevention and treatment of enteric infections. Immunol Res. 2000;21:1–6. doi: 10.1385/IR:21:1:1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Carlander D, Kollberg H, Larsson A. Retention of specific yolk IgY in the human oral cavity. BioDrugs. 2002;16:433–437. doi: 10.2165/00063030-200216060-00004. [DOI] [PubMed] [Google Scholar]
  10. Casswall T. Passive immunisation as therapy for gastrointestinal infections in children. Karolinska Institute, Stockholm: Doctoral Thesis; 1999. pp. 1–80. [Google Scholar]
  11. Chacana PA, Terzolo HR, Calzado EG, Schade R. Tecnologia IgYo applicaciones de los anticuerpos de yema de huevo de gallina. Rev Med Veterinaria. 2004;85:179–189. [Google Scholar]
  12. Chang HM, Ou-Yang RF, Chen YT, Chen CC. Productivity and some properties of immunoglobulin specific against Streptococcus mutans serotype c in chicken egg yolk (IgY) J Agric Food Chem. 1999;47:61–66. doi: 10.1021/jf980153u. [DOI] [PubMed] [Google Scholar]
  13. Cova L. DNA-designed avian IgY antibodies: novel tools for research, diagnostics and therapy. J Clin Virol 34 Suppl. 2005;1:S70–S74. doi: 10.1016/S1386-6532(05)80013-7. [DOI] [PubMed] [Google Scholar]
  14. Danielpour D, Roberts AB. Specific and sensitive quantitation of transforming growth factor b3 by sandwich enzyme-linked immunosorbent assay. J Immun Methods. 1995;180:265–272. doi: 10.1016/0022-1759(94)00322-N. [DOI] [PubMed] [Google Scholar]
  15. De Ceuninck F, Pastoureau P, Agnellet S, Bonnet J, Vanhoutte PM. Development of an enzyme-linked immunoassay for the quantification of YKL-40 (cartilage gp-39) in guinea pig serum using hen egg yolk antibodies. J Immunol Methods. 2001;252:153–161. doi: 10.1016/S0022-1759(01)00352-0. [DOI] [PubMed] [Google Scholar]
  16. Erhard MH, Bergmann J, Renner M, Hofmann A, Heinritzi K. Prophylactic effect of specific egg yolk antibodies in diarrhoea of weaned piglets caused by Escherichia coli K88 (F4) J Vet Med A. 1996;43:217–223. doi: 10.1111/j.1439-0442.1996.tb00447.x. [DOI] [PubMed] [Google Scholar]
  17. Finlay WJJ, deVore NC, Dobrovolskaia EN, Goodyear CS, Slater JE. Exploiting the avian immunoglobulin system to simplify the generation of recombinant antibodies to allergenic proteins. Clin Exp Allergy. 2005;35:1040–1048. doi: 10.1111/j.1365-2222.2005.02307.x. [DOI] [PubMed] [Google Scholar]
  18. Gerl M, Steinert C, Quint M, Schade R, Günzler V. Immunisation of chickens with the aminoterminal propeptide of bovine procollagen type III. ALTEX 13 Suppl. 1996;1:51–56. [PubMed] [Google Scholar]
  19. Gomez CM, Biamonte S, Barragan J, Babusci M, Garavano J, Guillen G, Schade R. Production of chicken antibodies against botulinic toxin A. Adv Agric Sci. 2006;10:25–32. [Google Scholar]
  20. Hamada S, Kodoma Y. Passive immunity for protection against mucosal infections and vaccination for dental caries. In: Kiyono H, Ogra PL, McGhee JR, editors. Mucosal vaccines. New York: Academic Press; 1996. pp. 187–197. [Google Scholar]
  21. Hatta H, Mabe K, Kim M, Yamamoto T, Guiterrez MA, Miyazaki T. Prevention of fish disease using egg yolk antibody. In: Sim JS, Nakai S, editors. Egg uses and processing technologies. Wallingford, UK: CAB International; 1994. pp. 241–249. [Google Scholar]
  22. Hatta H, Tsuda K, Ozeki M, Kim M, Yamamoto T, Otake S, Hirasawa M, Katz J, Childers NK, Michalek SM. Passive immunization against dental plaque formation in humans: effect of a mouth rinse containing egg yolk antibodies (IgY) specific to Streptococcus mutans. Caries Res. 1997;31:268–274. doi: 10.1159/000262410. [DOI] [PubMed] [Google Scholar]
  23. Hau J, Hendriksen CFM. Refinement of polyclonal antibody production by combining oral immunization of chickens with harvest of antibodies from the egg yolk. ILAR. 2005;46:294–299. doi: 10.1093/ilar.46.3.294. [DOI] [PubMed] [Google Scholar]
  24. Hinerfeld D, Innamorati D, Pirro J, Tam SW. Serum/plasma depletion with chicken immunoglobulin Y antibodies for proteomic analysis from multiple mammalian species. J Biomol Tech. 2004;15:184–190. [PMC free article] [PubMed] [Google Scholar]
  25. Horie K, Horie N, Abdou AM, Yang JO, Yun SS, Chun HN, Park CK, Kim M, Hatta H. Suppressive effect of functional drinking yogurt containing specific egg yolk immunoglobulin on Helicobacter pylori in humans. J Dairy Sci. 2004;87:4073–4079. doi: 10.3168/jds.S0022-0302(04)73549-3. [DOI] [PubMed] [Google Scholar]
  26. Huang L, Harvie G, Feitelson JS, Gramatikoff K, Herold DA, Allen DL, Amunngama R, Hagler RA, Pisano MR, Zhang W-W, Fang X. Immunoaffinity separation of plasma proteins by IgY microbeads: Meeting the needs of proteomic sample preparation and analysis. Proteomics. 2005;5:3314–3328. doi: 10.1002/pmic.200401277. [DOI] [PubMed] [Google Scholar]
  27. Karlsson M, Kollberg H, Larsson A. Chicken IgY: utilizing the evolutionary advantage. World's Poultry Sci J. 2004;60:341–347. doi: 10.1079/WPS200422. [DOI] [Google Scholar]
  28. Kim DK, Jang IK, Seo HC, Shin SO, Yang SY, Kim JW. Shrimp protected from WSSV disease by treatment with egg yolk antibodies (IgY) against a truncated fusion protein derived from WSSV. Aquaculture. 2004;237:21–30. doi: 10.1016/j.aquaculture.2004.03.015. [DOI] [Google Scholar]
  29. Klemperer F. Ueber natürliche Immunität und ihre Verwerthung für die Immuni-sierungstherapie. Arch Exp Pathol Pharmakol. 1893;31:356–382. doi: 10.1007/BF01832882. [DOI] [Google Scholar]
  30. Kollberg H, Carlander D, Olesen H, Wejaker P-E, Johannesson M, Larsson A. Oral administration of specific yolk antibodies (IgY) may prevent Pseudomonas aeruginosa infections in patients with cystic fibrosis: a phase I feasibility study. Pediatr Pulmonol. 2003;35:433–440. doi: 10.1002/ppul.10290. [DOI] [PubMed] [Google Scholar]
  31. Kovacs-Nolan J, Mine Y. Passive immunization through avian egg antibodies. Food Biotechnol. 2004;18:39–62. doi: 10.1081/FBT-120030384. [DOI] [Google Scholar]
  32. Kuroki M, Ohta M, Ikemori Y, Icatlo FC, Kobayashi C, Yokoyama H, Kodama Y. Field evaluation of chicken egg yolk immunoglobulins specific for bovine rotavirus in neonatal calves. Arch Virol. 1997;142:843–851. doi: 10.1007/s007050050123. [DOI] [PubMed] [Google Scholar]
  33. Larsson A, Karlsson-Parra A, Sjöquist J. Use of chicken antibodies in enzyme immunoassays to avoid interference by rheumatoid factors. Clin Chem. 1991;37:411–414. [PubMed] [Google Scholar]
  34. Larsson A, Wejaker P-E, Forsberg P-O, Lindahl T. Chicken antibodies: a tool to avoid interference by complement activation in ELISA. J Immunol Methods. 1992;156:79–83. doi: 10.1016/0022-1759(92)90013-J. [DOI] [PubMed] [Google Scholar]
  35. LeClaire RD, Hunt RE, Bavari S. Protection against bacterial staphylococcal enterotoxin B by passive vaccination. Infect Immun. 2002;70:2278–2281. doi: 10.1128/IAI.70.5.2278-2281.2002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Lee SB, Mine Y, Stevenson MW. Effects of hen egg yolk immunoglobulin in passive protection of rainbow trout against Yersinia ruckeri. J Agric Food Chem. 2000;48:110–115. doi: 10.1021/jf9906073. [DOI] [PubMed] [Google Scholar]
  37. Lee EN, Sunwoo HH, Menninen K, Sim JS. In vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium. Poultry Science. 2002;81:632–641. doi: 10.1093/ps/81.5.632. [DOI] [PubMed] [Google Scholar]
  38. Leenars PPAM, Hendriksen CFM, de Leeuw WA, Carat F, Delahaut P, Fischer R, Halder M, Hanly WC, Hartinger J, Hau J, Lindblad EB, Nicklas W, Outschoorn IM, Stewart-Tull DES. The production of polyclonal antibodies in laboratory animals. The report and recommendations of ECVAM workshop 35. ATLA. 1999;27:79–102. doi: 10.1177/026119299902700106. [DOI] [PubMed] [Google Scholar]
  39. Lemley PV, Thalley BS, Stafford DC. Prophylactic and therapeutic efficacy of an avian antitoxin in ricin intoxication. Therap Immunol. 1995;2:59–66. [PubMed] [Google Scholar]
  40. Lösch U, Schranner I, Wanke R, Jürgens L. The chicken egg: an antibody source. J Vet Med B. 1986;33:609–619. doi: 10.1111/j.1439-0450.1986.tb00076.x. [DOI] [PubMed] [Google Scholar]
  41. Marquardt RR, Jin LZ, Jung-Woo K, Fang L, Frohlich AA, Baidoo SK. Passive protective effect of egg-yolk antibodies against enterotoxigenic Escherichia coli K88+ infection in neonatal and early weaned piglets. FEMS Immunol Med Microbiol. 1999;23:283–88. doi: 10.1016/S0928-8244(98)00147-3. [DOI] [PubMed] [Google Scholar]
  42. Mine Y, Kovacs-Nolan J. Chicken egg yolk antibodies as therapeutics in enteric infectious disease: a review. J Med Food. 2002;5:159–169. doi: 10.1089/10966200260398198. [DOI] [PubMed] [Google Scholar]
  43. Nakamura N, Shuyama A, Hojyo S, Shimokawa M, Miyamoto K, Kawashima T, Aosasa M, Horiuchi H, Furusawa S, Matsuda H. Establishment of a chicken monoclonal antibody panel against mammalian prion protein. J Vet Med Sci. 2004;66:807–814. doi: 10.1292/jvms.66.807. [DOI] [PubMed] [Google Scholar]
  44. Narat M. Production of antibodies in chickens. Food Technol Biotechnol. 2003;41:259–267. [Google Scholar]
  45. Nomura S, Suzuki H, Masaoka T, Kurabayashi K, Ishii H, Kitajima M, Nomoto K, Hibi T. Effect of dietary anti-urease immunoglobulin Y on Helicobacter pylori infection in Mongolian gerbils. Helicobacter. 2005;10:43–52. doi: 10.1111/j.1523-5378.2005.00290.x. [DOI] [PubMed] [Google Scholar]
  46. Otake S, Nishihara Y, Makimura M, Hatta H, Kim M, Yamamoto T, Hirasawa M. Protection of rats against dental caries by passive immunization with hen-egg-yolk antibody (IgY) J Dent Res. 1991;70:162–166. doi: 10.1177/00220345910700030101. [DOI] [PubMed] [Google Scholar]
  47. Park KJ, Park DW, Kim CH, Han BK, Park TS, Han JY, Lillehoj HS, Kim J-K. Development and characterization of a recombinant chicken single-chain Fv antibody detecting Eimeria acervulina sporozoite antigen. Biotechnol Letters. 2005;27:289–295. doi: 10.1007/s10529-005-0682-8. [DOI] [PubMed] [Google Scholar]
  48. Pichler H, Krska R, Szekacs A, Grasserbauer M. An enzyme-immunoassay for the detection of the mycotoxin zearalenone by use of yolk antibodies. Fresenius J Analyt Chem. 1998;362:176–177. doi: 10.1007/s002160051053. [DOI] [Google Scholar]
  49. Pokorova D, Franz J, Stepanek J. The use of egg yolk immunoglobulin in the diagnostics of canine parvovirus infections. Veterinarni Medicina. 2000;45:49–54. [Google Scholar]
  50. Rosol TJ, Steinmeyer CL, McCauley LK, Merryman JI, Werkmeister JR, Gröne A, Weckman MT, Swayne DE, Capen CC. Studies on chicken polyclonal anti-peptide antibodies specific for parathyroid hormone-related protein (1–36) Vet Immunol Immunopathol. 1993;35:321–337. doi: 10.1016/0165-2427(93)90042-3. [DOI] [PubMed] [Google Scholar]
  51. Sarker SA, Casswall TH, Juneja LR, Hoq E, Hossain I, Fuchs GJ, Hammarström L. Randomized, placebo-controlled, clinical trial of hyperimmunized chicken egg yolk immunoglobulin in children with rotavirus diarrhea. J Ped Gastroenterol Nutr. 2001;32:9–25. doi: 10.1097/00005176-200101000-00009. [DOI] [PubMed] [Google Scholar]
  52. Schade R, Behn I, Erhard M, Hlinak A, Staak C. Chicken egg yolk antibodies, production and application: IgY technology. Berlin Heidelberg New York: Springer Lab Manuals; 2000. pp. 1–255. [Google Scholar]
  53. Schade R, Calzado EG, Sarmiento R, Chacana PA, Porankiewicz-Asplund J, Terzolo HR. Chicken egg yolk antibodies (IgY-technology): A review of progress in production and use in research and human and veterinary medicine. ATLA. 2005;33:129–154. doi: 10.1177/026119290503300208. [DOI] [PubMed] [Google Scholar]
  54. Schniering A, Schade R, Hiepe T. Development of an IgY-based assay for the detection of Ascaris suum antigens. ALTEX 13 Suppl. 1996;1:62–65. [PubMed] [Google Scholar]
  55. Shimamoto C, Tokioka S, Hirata I, Tani H, Ohishi H, Katsu K. Inhibition of Helicobacter pylori infection by orally administered yolk derived anti-Helicobacter pylori antibody. Hepatogastroenterology. 2002;49:709–714. [PubMed] [Google Scholar]
  56. Shin JH, Nam SW, Kim JT, Yoon JB, Bang WG, Roe IH. Identification of immunodominant Helicobacter pylori proteins with reactivity to H. pylori-specific egg-yolk immunoglobulin. J Med Microbiol. 2003;52:217–222. doi: 10.1099/jmm.0.04978-0. [DOI] [PubMed] [Google Scholar]
  57. Smith DJ, King WF, Godiska R. Passive transfer of immunoglobulin Y antibody to Streptococcus mutans glucan binding protein B can confer protection against experimental dental caries. Infect Immun. 2001;69:3135–3142. doi: 10.1128/IAI.69.5.3135-3142.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  58. Sunwoo HH, Sim JS. IgY as ovo-nutraceuticals: Anti-gluten specific IgY enforced egg products for celiac disease. The 3rd international symposium on egg nutrition for health promotion. Canada: Banff, Alberta; 2004. pp. 18–21. [Google Scholar]
  59. Sunwoo HH, Lee EN, Menninen K, Suresh MR, Sim JS. Growth inhibitory effect of chicken egg yolk antibody (IgY) on Escherichia coli 0157:H7. J Food Sci. 2002;67:1486–1494. doi: 10.1111/j.1365-2621.2002.tb10310.x. [DOI] [Google Scholar]
  60. Suzuki H, Nomura S, Masaoka T, Goshima H, Kamata N, Kodama Y, Ishii H, Kitajima M, Nomoto K, Hibi T. Effect of dietary anti-Helicobacter pylori-urease immunoglobulin Y on. Aliment Pharmacol Ther 20 Suppl. 2004;1:185–192. doi: 10.1111/j.1365-2036.2004.02027.x. [DOI] [PubMed] [Google Scholar]
  61. Terzolo H, Chacana P, Vivas A, Greco C, Goya R, Schade R. IgY technology in Argentina. ALTEX. 2003;20:202–203. [Google Scholar]
  62. Thalley BS, Carroll SB. Rattlesnake and scorpion antivenoma from the egg yolks of immunized hens. Biotechnol. 1990;8:934–938. doi: 10.1038/nbt1090-934. [DOI] [PubMed] [Google Scholar]
  63. Tsurushita N, Park M, Pakabunto K, Ong K, Avdalovic A, Fu H, Jia A, Vásquez M, Kumar S. Humanization of a chicken anti-IL-12 monoclonal antibody. J Immunol Methods. 2004;295:9–19. doi: 10.1016/j.jim.2004.08.018. [DOI] [PubMed] [Google Scholar]
  64. Wiedemann V, Linckh E, Kühlmann R, Schmidt P, Lösch U. Chicken egg antibodies for prophylaxis and therapy of infectious intestinal disorders. V. In vivo studies on protective effects against Escherichia coli diarrhea in pigs. J Vet Med Series B. 1991;38:283–291. doi: 10.1111/j.1439-0450.1991.tb00872.x. [DOI] [PubMed] [Google Scholar]
  65. Worledge KL, Godiska R, Barrett TA, Kink JA. Oral administration of avian tumor necrosis factor antibodies effectively treats experimental colitis in rats. Digest Dis Sci. 2000;45:2298–2305. doi: 10.1023/A:1005554900286. [DOI] [PubMed] [Google Scholar]
  66. Yolken RH, Leister F, Wee S-B, Miskuff R, Vonderfecht S. Antibodies to rotaviruses in chicken's eggs: a potential source of antiviral immunoglobulins suitable for human consumption. Pediatrics. 1988;81:291–295. [PubMed] [Google Scholar]
  67. Yu QS, Wang GP, Wang W, Qin Y, Huang S. Anti-King cobra egg yolk antibody from the egg yolks of immunized hens and its primary application. Chin J Microbiol Immunol. 2004;24:695–698. [Google Scholar]
  68. Zhou Z, Zhou R, Tang Z. Effects of topical application of immunoglobulin yolk on mutans streptococci in dental plaque. Hua Xi Kou Quiang Yi Xue Za Zhi. 2003;21:295–297. [PubMed] [Google Scholar]

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