Abstract
Newly emerging and re-emerging infections are recognized as a global problem and 75% of these are potentially zoonotic (Woolhouse & Gowtage-Sequeria, 2005). Emergence of a new “killer” disease in any part of the world is likely to be a threat world wide in today’s society with very rapid means of transportation of both human and animal/animal products. Recent examples include the global outbreaks of severe acute respiratory syndrome (SARS), H5N1 avian influenza, and the outbreaks of West Nile virus in United States. The rapid economic development in the Asian region during the last few decades was accompanied by massive urbanization and environmental changes, which are believed to be one of the triggers leading to the emergence of new zoonotic diseases. Wildlife animals play an ever-increasing role in the emergence of zoonotic diseases, and bats have been identified as natural reservoir host of several lethal zoonotic viruses that emerged in recent times. This review will focus on the disease outbreaks caused by emerging bat viruses in the family Paramyxoviridae.
Keywords: Severe Acute Respiratory Syndrome, Severe Acute Respiratory Syndrome, Nipah Virus, Hendra Virus, Severe Acute Respiratory Syndrome Coronavirus
Contributor Information
Yichen Lu, Email: yichenlu@hsph.harvard.edu.
M. Essex, Email: messex@hsph.harvard.edu
Bryan Roberts, Email: bryanr1@comcast.net.
Lin-Fa Wang, Email: Linfa.Wang@csiro.au.
John S. Mackenzie, Email: j.mackenzie@curtin.edu.au
Bryan T. Eaton, Email: Bryan.eaton@csiro.au
References
- Bossart KN, Broder CC. Developments towards effective treatments for Nipah and Hendra virus infection. Expert Review of Anti-infective Therapy. 2006;4:43–55. doi: 10.1586/14787210.4.1.43. [DOI] [PubMed] [Google Scholar]
- Bossart KN, Crameri G, Dimitrov AS, Mungall B, Feng YR, Patch JR, Choudhary A, Wang LF, Eaton BT, Broder CC. Receptor-binding, fusion inhibition and induction of cross-reactive neutralizing antibodies by a soluble G glycoprotein of Hendra virus. Journal of Virology. 2005;79:6690–6702. doi: 10.1128/JVI.79.11.6690-6702.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bossart KN, Mungall BA, Crameri GC, Wang LF, Eaton BT, Broder CC. Inhibition of henipavirus fusion and infection by heptad-derived peptides of the Nipah virus fusion protein. Virology Journal. 2005;2:57. doi: 10.1186/1743-422X-2-57. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bowden TR, Boyle DB. Completion of the full-length genome sequence of Menangle virus: Characterisation of the polymerase gene and genomic 5 trailer region. Archives of Virology. 2005;150:2125–2137. doi: 10.1007/s00705-005-0552-7. [DOI] [PubMed] [Google Scholar]
- Bowden TR, Westenberg M, Wang LF, Eaton BT, Boyle DB. Molecular characterization of Menangle virus, a novel paramyxovirus which infects pigs, fruit bats, and humans. Virology. 2001;283:358–373. doi: 10.1006/viro.2001.0893. [DOI] [PubMed] [Google Scholar]
- Chadha MS, Comer JA, Lowe L, Rota PA, Rollin PE, Bellini WJ, Ksiazek TG, Mishra AC. Nipah virus identified as the agent responsible for an outbreak of encephalitis in Siliguri, India. Emerging Infectious Diseases. 2006;12:235–240. doi: 10.3201/eid1202.051247. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chant K, Chan R, Smith M, Dwyer DE, Kirkland P. Probable human infection with a newly described virus in the family Paramyxoviridae. Emerging Infectious Diseases. 1998;4:273–275. doi: 10.3201/eid0402.980215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chong HT, Kamarulzaman A, Tan CT, Goh KJ, Thayaparan T, Kunjapan R, Chew NK, Chua KB, Lam SK. Treatment of acute Nipah encephalitis with ribavirin. Annals of Neurology. 2001;49:810–813. doi: 10.1002/ana.1062. [DOI] [PubMed] [Google Scholar]
- Chua KB. Nipah virus outbreak in Malaysia. Journal of Clinical Virology. 2003;26:265–275. doi: 10.1016/S1386-6532(02)00268-8. [DOI] [PubMed] [Google Scholar]
- Chua KB, Bellini WJ, Rota PA, Harcourt BH, Tamin A, Lam SK, Ksiazek TG, Rollin PE, Zaki SR, Shieh WJ, Goldsmith CS, Gubler DJ, Roehrig JT, Eaton B, Gould AR, Olson J, Field H, Daniels P, Ling AE, Peters CJ, Anderson LJ, Mahy BWJ. Nipah virus: A recently emergent deadly paramyxovirus. Science. 2000;288:1432–1435. doi: 10.1126/science.288.5470.1432. [DOI] [PubMed] [Google Scholar]
- Chua KB, Goh KJ, Wong KT, Kamarulzaman A, Tan PSK, Ksiazek TG, Zaki SR, Paul G, Lam SK, Tan CT. Fatal encephalitis due to Nipah virus among pig-farmers in Malaysia. Lancet. 1999;354:1257–1259. doi: 10.1016/S0140-6736(99)04299-3. [DOI] [PubMed] [Google Scholar]
- Chua KB, Koh CL, Hooi PS, Wee KF, Khong JH, Chua BH, Chan YP, Lim ME, Lam Sai Kit K. Isolation of Nipah virus from Malaysian island flying-foxes. Microbes and Infection. 2002;4:145–151. doi: 10.1016/S1286-4579(01)01522-2. [DOI] [PubMed] [Google Scholar]
- Chua KB, Lam SK, Goh KJ, Hooi PS, Ksiazek TG, Kamarulzaman A, Olson J, Tan CT. The presence of Nipah virus in respiratory secretions and urine of patients during an outbreak of Nipah virus encephalitis in Malaysia. Journal of Infection. 2001;42:40–43. doi: 10.1053/jinf.2000.0782. [DOI] [PubMed] [Google Scholar]
- Chua KB, Wang LF, Lam SK, Crameri G, Yu M, Wise T, Boyle D, Hyatt AD, Eaton BT. Tioman virus, a novel paramyxovirus isolated from fruit bats in Malaysia. Virology. 2001;283:215–229. doi: 10.1006/viro.2000.0882. [DOI] [PubMed] [Google Scholar]
- Chua KB, Wang LF, Lam SK, Eaton BT. Full length genome sequence of Tioman virus, a novel paramyxovirus in the genus Rubulavirus isolated from fruit bats in Malaysia. Archives of Virology. 2002;147:1323–1348. doi: 10.1007/s00705-002-0815-5. [DOI] [PubMed] [Google Scholar]
- Daniels P, Aziz J, Ksiazek T, Ong B, Bunning M, Johara B, Field H, Olson J, Hoffmann D, Bilou J, Ozawa Y. Nipah virus considerations for regional preparedness. In: Blacksell S, editor. Classical Swine Fever and Emerging Diseases in Southeast Asia. ACIAR Proceedings No. 94. Canberra: Australian Centre for International Agricultural Research; 2000. pp. 133–141. [Google Scholar]
- Eaton BT, Broder CC, Middelton D, Wang LF. Hendra and Nipah viruses: Different and dangerous. Nature Reviews Microbiology. 2006;4:23–35. doi: 10.1038/nrmicro1323. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Field, H., Mackenzie, J., & Daszak, P. (2004). Novel viral encephalitides associated with bats (Chiroptera)–host management strategies. Archives of Virology Supplement, 113–121. [DOI] [PubMed]
- Field H, Young P, Yob JM, Mills J, Hall L, Mackenzie J. The natural history of Hendra and Nipah viruses. Microbes and Infection. 2001;3:307–314. doi: 10.1016/S1286-4579(01)01384-3. [DOI] [PubMed] [Google Scholar]
- Field HE, Hall LS, Mackenzie JS. Emergence and Control of Zoonotic Ortho and Paramyxovirus Diseases. Montrouge France: John Libby Eurotext; 2001. Emerging zoonotic paramyxoviruses: The role of pteropid bats; pp. 205–209. [Google Scholar]
- Field HF, Mackenzie JS, Daszak P. Henipaviruses: Emerging paramyxoviruses associated with fruit bats. Current Topics in Microbiology and Immunology. 2007;315:133–159. doi: 10.1007/978-3-540-70962-6_7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goh KJ, Tan CT, Chew NK, Tan PSK, Kamarulzaman A, Sarji SA, Wong KT, Abdullah BJJ, Chua KB, Lam SK. Clinical features of Nipah virus encephalitis among pig farmers in Malaysia. New England Journal of Medicine. 2000;342:1229–1235. doi: 10.1056/NEJM200004273421701. [DOI] [PubMed] [Google Scholar]
- Griffin DE. Measles viruses. In: Fields BN, Knipe DM, Howley PM, editors. Fields Virolog. 4. Philadelphia: Lippincott-Raven; 2001. pp. 1401–1442. [Google Scholar]
- Guillaume V, Contamin H, Loth P, Grosjean I, Georges Courbot MC, Deubel V, Buckland R, Wild TF. Antibody prophylaxis and therapy against Nipah virus infection in hamsters. Journal of Virology. 2006;80:1972–1978. doi: 10.1128/JVI.80.4.1972-1978.2006. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall L, Richards G. Flying foxes. Sydney: University of New South Wales; 2000. [Google Scholar]
- Halpin K, Young PL, Field HE, Mackenzie JS. Isolation of Hendra virus from pteropid bats: A natural reservoir of Hendra virus. Journal of General Virology. 2000;81:1927–1932. doi: 10.1099/0022-1317-81-8-1927. [DOI] [PubMed] [Google Scholar]
- Harcourt BH, Lowe L, Tamin A, Liu X, Bankamp B, Bowden N, Rollin PE, Comer JA, Ksiazek TG, Hossain MJ, Gurley ES, Brieman RF, Bellini WJ, Rota PA. Genetic characterization of Nipah virus, Bangladesh, 2004. Emerging Infectious Diseases. 2005;11:1594–1597. doi: 10.3201/eid1110.050513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hooper PT, Gould AR, Russell GM, Kattenbelt JA, Mitchell G. The retrospective diagnosis of a second outbreak of equine morbillivirus infection. Australian Veterinary Journal. 1996;74:244–245. doi: 10.1111/j.1751-0813.1996.tb15414.x. [DOI] [PubMed] [Google Scholar]
- Hooper PT, Ketterer PJ, Hyatt AD, Russell GM. Lesions of experimental equine morbillivirus pneumonia in horses. Veterinary Pathology. 1997;34:312–322. doi: 10.1177/030098589703400407. [DOI] [PubMed] [Google Scholar]
- Hsu VP, Hossain MJ, Parashar UD, Ali MM, Ksiazek TG, Kuzmin I, Niezgoda M, Rupprecht C, Brese J, Brieman RF. Nipah virus encephalitis reemergence, Bangladesh. Emerging Infectious Diseases. 2004;12:2082–2087. doi: 10.3201/eid1012.040701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ICDDRB Outbreaks of viral encephalitis due to Nipah/Hendra-like viruses, Western Bangladesh. HSB Health and Science Bulletin. 2003;1:1–6. [Google Scholar]
- ICDDRB Nipah encephalitis outbreak over wide area of Western Bangladesh, 2004. Health and Science Bulletin. 2004;2:7–11. [Google Scholar]
- ICDDRB Person to person transmission of Nipah virus during outbreak in Faridpur district 2004. Health and Science Bulletin. 2004;2:5–9. [Google Scholar]
- ICDDRB Nipah virus outbreak from date palm juice. Health and Science Bulletin. 2005;3:1–5. [Google Scholar]
- Koopman KF. Order Chiroptera. In: Wilson DE, Reeder DM, editors. Mammal Species of the World: A Taxonomy and Geographic Reference. 2. Washington: Smithsonian Institution Press; 1992. pp. 137–241. [Google Scholar]
- Kumar S. Inadequate research facilities fail to tackle mystery disease. British Medical Journal. 2003;326:12. doi: 10.1136/bmj.326.7379.12/d. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lau SKP, Woo PCY, Li KSM, Huang Y, Tsoi H, Wong BHL, Wong SSY, Leung S, Chan K, Yuen K. Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats. Proceedings of National Academy of Sciences USA. 2005;102:14040–14045. doi: 10.1073/pnas.0506735102. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leroy EM, Kumulungui B, Pourrut X, Rouquet P, Hassanin A, Yaba P, Delicat A, Paweska JT, Gonzalez JP, Swanepoel R. Fruit bats as reservoirs of Ebola virus. Nature. 2005;438:575–576. doi: 10.1038/438575a. [DOI] [PubMed] [Google Scholar]
- Li W, Shi Z, Yu M, Ren W, Smith C, Epstein JH, Wang H, Crameri G, Hu Z, Zhang H, Jianhong Z, McEachern J, Field H, Daszak P, Eaton BT, Zhang S, Wang LF. Bats are natural reservoirs of SARS-like coronaviruses. Science. 2005;310:676–679. doi: 10.1126/science.1118391. [DOI] [PubMed] [Google Scholar]
- Love RJ, Philbey AW, Kirkland PD, Ross AD, Davis RJ, Morrissey C, Daniels PW. Reproductive disease and congenital malformations caused by Menangle virus in pigs. Australian Veterinary Journal. 2001;79:192–198. doi: 10.1111/j.1751-0813.2001.tb14578.x. [DOI] [PubMed] [Google Scholar]
- Mackenzie, J. S., & Field, H. E. (2004). Emerging encephalitogenic viruses: lyssaviruses and henipaviruses transmitted by frugivorous bats. Archives of Virology Supplement, 97–111. [DOI] [PubMed]
- Mackenzie JS, Field HE, Guyatt KJ. Managing emerging diseases borne by fruit bats (flying foxes), with particular reference to henipaviruses and Australian bat lyssavirus. Journal of Applied Microbiology. 2003;94:59S–69S. doi: 10.1046/j.1365-2672.94.s1.7.x. [DOI] [PubMed] [Google Scholar]
- Mayo MA. Virus taxonomy – Houston 2002. Archives of Virology. 2002;147:1071–1076. doi: 10.1007/s007050200036. [DOI] [PubMed] [Google Scholar]
- Middleton DJ, Westbury HA, Morrissy CJ, van der Heide BM, Russell GM, Braun MA, Hyatt AD. Experimental Nipah virus infection in pigs and cats. Journal of Comparative Pathology. 2002;126:124–136. doi: 10.1053/jcpa.2001.0532. [DOI] [PubMed] [Google Scholar]
- Mohd. Nor MN, Gan CH, Ong BL. Nipah virus infection of pigs in peninsular Malaysia. Revue Scientific Et Technical Office Internationale Epizootie. 2000;19:160–165. doi: 10.20506/rst.19.1.1202. [DOI] [PubMed] [Google Scholar]
- Mounts AW, Kaur H, Parashar UD, Ksiazek TG, Cannon D, Arokiasamy JT, Anderson LJ, Lye MS. A cohort study of health care workers to assess nosocomial transmissibility of Nipah virus, Malaysia, 1999. Journal of Infectious Disease. 2001;183:810–813. doi: 10.1086/318822. [DOI] [PubMed] [Google Scholar]
- Murray K, Rogers R, Selvey L, Selleck P, Hyatt A, Gould A, Gleeson L, Hooper P, Westbury H. A novel morbillivirus pneumonia of horses and its transmission to humans. Emerging Infectious Diseases. 1995;1:31–33. doi: 10.3201/eid0101.950107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murray K, Selleck P, Hooper P, Hyatt A, Gould A, Gleeson L, Westbury H, Hiley L, Selvey L, Rodwell B. A morbillivirus that caused fatal disease in horses and humans. Science. 1995;268:94–97. doi: 10.1126/science.7701348. [DOI] [PubMed] [Google Scholar]
- O’Sullivan JD, Allworth AM, Paterson DL, Snow TM, Boots R, Gleeson LJ, Gould AR, Hyatt AD, Bradfield J. Fatal encephalitis due to novel paramyxovirus transmitted from horses. Lancet. 1997;349:93–95. doi: 10.1016/S0140-6736(96)06162-4. [DOI] [PubMed] [Google Scholar]
- Olson JG, Rupprecht C, Rollin PE, An US, Niezgoda M, Clemins T, Walston J, Ksiazek TG. Antibodies to Nipah-like virus in bats (Pteropus lylei), Cambodia. Emerging Infectious Diseases. 2002;8:987–988. doi: 10.3201/eid0809.010515. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parashar UD, Sunn LM, Ong F, Mounts AW, Arif MT, Ksiazek TG, Kamaluddin MA, Mustafa AN, Kaur H, Ding LM, Othman G, Radzi HM, Kitsutani PT, Stockton PC, Arokiasamy JT, Gary HE, Jr, Anderson LJ. Case-control study of risk factors for human infection with a new zoonotic paramyxovirus, Nipah virus, during a 1998–1999 outbreak of severe encephalitis in Malaysia. Journal of Infectious Disease. 2000;181:1755–1759. doi: 10.1086/315457. [DOI] [PubMed] [Google Scholar]
- Paton NI, Leo YS, Zaki SR, Auchus AP, Lee KE, Ling AE, Chew SK, Ang BSP, Rollin PE, Umapathi T, Sng I, Lee CC, Lim E, Ksiazek TG. Outbreak of Nipah-virus infection among abattoir workers in Singapore. Lancet. 1999;354:1253–1256. doi: 10.1016/S0140-6736(99)04379-2. [DOI] [PubMed] [Google Scholar]
- Philbey AW, Kirkland PD, Ross AD, Davis RJ, Gleeson AB, Love RJ, Daniels PW, Gould AR, Hyatt AD. An apparently new virus (family Paramyxoviridae) infectious for pigs, humans, and fruit bats. Emerging Infectious Diseases. 1998;4:269–271. doi: 10.3201/eid0402.980214. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pringle CR. The order Mononegavirales. Archives of Virology. 1991;117:137–140. doi: 10.1007/BF01310499. [DOI] [PubMed] [Google Scholar]
- ProMed. (2004). Hendra virus – Australia (Queensland). Archive Number 20041214.3307.
- Reynes J, Counor D, Ong S, Faure C, Seng V, Molia S, Walston J, Georges-Coubot MC, Deubel V, Sarthou J. Nipah virus in Lyle’s flying foxes, Cambodia. Emerging Infectious Diseases. 2005;11:1042–1047. doi: 10.3201/eid1107.041350. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rogers RJ, Douglas IC, Baldock FC, Glanville RJ, Seppanen KT, Gleeson LJ, Selleck PN, Dunn KJ. Investigation of a second focus of equine morbillivirus infection in coastal Queensland. Australian Veterinary Journal. 1996;74:243–244. doi: 10.1111/j.1751-0813.1996.tb15413.x. [DOI] [PubMed] [Google Scholar]
- Selvey L, Taylor R, Arklay A, Gerrard J. Screening of bat carers for antibodies to equine morbillivirus. Communiccable Diseases Intelligence. 1996;20:477–478. [Google Scholar]
- Selvey LA, Wells RM, McCormack JG, Ansford AJ, Murray K, Rogers RJ, Lavercombe PS, Selleck P, Sheridan JW. Infection of humans and horses by a newly described morbillivirus. Medical Journal of Australia. 1995;162:642–645. doi: 10.5694/j.1326-5377.1995.tb126050.x. [DOI] [PubMed] [Google Scholar]
- Sendow I, Field HF, Curran J, Morrissy C, Meehan G, Buick T, Daniels P. Henipavirus infection in Pteropus bats flying foxes in the Indonesian archipelago. Emerging Infectious Diseases. 2006;12:711–712. doi: 10.3201/eid1204.051181. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sulkin SE, Allen R. Virus infections in bats. In: Melnick JL, editor. Monographs in Virology. 8. Basel: Karger; 1974. [PubMed] [Google Scholar]
- Wacharapluesadee S, Lumlertdacha B, Boongird K, Wanghongsa S, Chanhome L, Rollin P, Stockton P, Rupprecht CE, Ksiazek TG, Hemachudha T. Bat Nipah virus, Thailand. Emerging Infectious Diseases. 2005;11:1949–1951. doi: 10.3201/eid1112.050613. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang LF, Yu M, Hansson E, Pritchard LI, Shiell B, Michalski WP, Eaton BT. The exceptionally large genome of Hendra virus: Support for creation of a new genus within the family Paramyxoviridae. Journal of Virology. 2000;74:9972–9979. doi: 10.1128/JVI.74.21.9972-9979.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williamson MM, Hooper PT, Selleck PW, Gleeson LJ, Daniels PW, Westbury HA, Murray PK. Transmission studies of Hendra virus (equine morbillivirus) in fruit bats, horses and cats. Australian Veterinary Journal. 1998;76:813–818. doi: 10.1111/j.1751-0813.1998.tb12335.x. [DOI] [PubMed] [Google Scholar]
- Williamson MM, Hooper PT, Selleck PW, Westbury HA, Slocombe RF. Experimental Hendra virus infection in pregnant guinea-pigs and fruit bats (Pteropus poliocephalus) Journal of Comparative Pathology. 2000;122:201–207. doi: 10.1053/jcpa.1999.0364. [DOI] [PubMed] [Google Scholar]
- Wong KT, Grosjean I, Brisson C, Blanquier B, Fevre-Montange M, Bernard A, Loth P, Georges-Courbot MC, Chevallier M, Akaoka H, Marianneau P, Lam SK, Wild TF, Deubel V. A golden hamster model for human acute Nipah virus infection. American Journal of Pathology. 2003;163:2127–2137. doi: 10.1016/S0002-9440(10)63569-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woolhouse MEJ, Gowtage-Sequeria S. Host range and emerging and reemerging pathogens. Emerging Infectious Diseases. 2005;11:1842–1847. doi: 10.3201/eid1112.050997. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wright PJ, Crameri GS, Eaton BT. RNA synthesis during infection by Hendra virus: An examination by quantitative real-time PCR of RNA accumulation, the effect of ribavirin and the attenuation of transcription. Archives of Virology. 2004;150:521–532. doi: 10.1007/s00705-004-0417-5. [DOI] [PubMed] [Google Scholar]
- Yob JM, Field H, Rashdi AM, Morrissy C, van der heide B, Rota P, Binadzhar A, White J, Daniels P, Jamaluddin A, Ksiazek T. Nipah virus infection in bats (order Chiroptera) in Peninsular Malaysia. Emerging Infectious Diseases. 2001;7:439–441. doi: 10.3201/eid0703.010312. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Young P, Halpin KFH, Mackenzie J. Finding the wild life reservoir of equine morbillivirus. Recent Advances in Microbiology. 1997;5:1–12. [Google Scholar]
- Young PL, Halpin K, Selleck PW, Field H, Gravel JL, Kelly MA, Mackenzie JS. Serologic evidence for the presence in Pteropus bats of a paramyxovirus related to equine morbillivirus. Emerging Infectious Diseases. 1996;2:239–240. doi: 10.3201/eid0203.960315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zhu Z, Dimitrov AS, Bossart KN, Crameri G, Bishop KA, Choudhry V, Mungall BA, Feng Y-R, Choudhary A, Zhang M-Y, Feng Y, Wang LF, Xiao X, Eaton BT, Broder CC, Dimitrov DS. Potent neutralization of Hendra and Nipah viruses by human monoclonal antibodies. Journal of Virology. 2006;80:891–899. doi: 10.1128/JVI.80.2.891-899.2006. [DOI] [PMC free article] [PubMed] [Google Scholar]
