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. 2006;581:323–326. doi: 10.1007/978-0-387-33012-9_57

Characterization of Persistent SARS-CoV Infection in Vero E6 Cells

Tetsuya Mizutani 3, Shuetsu Fukushi 4, Masayuki Saijo 5, Ichiro Kurane 6, Shigeru Morikawa 7
Editors: Stanley Perlman1, Kathryn V Holmes2
PMCID: PMC7122806  PMID: 17037553

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Contributor Information

Stanley Perlman, Email: Stanley-Perlman@uiowa.edu

Kathryn V. Holmes, Email: Kathryn.Holmes@ucHSC.edu

References

  • 1.Rota PA, Oberste MS, Monroe SS, et al. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science. 2003;300:1394–1399. doi: 10.1126/science.1085952. [DOI] [PubMed] [Google Scholar]
  • 2.Marra MA, Jones SJ, Astell CR, et al. The genome sequence of the SARS-associated coronavirus. Science. 2003;300:1399–1404. doi: 10.1126/science.1085953. [DOI] [PubMed] [Google Scholar]
  • 3.Chan PK, To KF, Lo AW, Cheung JL, Chu I, Au FW, Tong JH, Tam JS, Sung JJ, Ng HK. Persistent infection of SARS coronavirus in colonic cells in vitro. J. Med. Virol. 2004;74:1–7. doi: 10.1002/jmv.20138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Li W, Moore MJ, Vasilieva N, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature. 2003;426:450–454. doi: 10.1038/nature02145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Mizutani T, Fukushi S, Saijo M, Kurane I, Morikawa S. Phosphorylation of p38 MAPK and its downstream targets in SARS coronavirus-infected cells. Biochem. Biophys. Res. Commun. 2004;319:1228–1234. doi: 10.1016/j.bbrc.2004.05.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Mizutani T, Fukushi S, Saijo M, Kurane I, Morikawa S. Importance of Akt signaling pathway for apoptosis in SARS-CoV-infected Vero E6 cells. Virology. 2004;327:169–174. doi: 10.1016/j.virol.2004.07.005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Mizutani T, Fukushi S, Murakami M, Hirano T, Saijo M, Kurane I, Morikawa S. Tyrosine dephosphorylation of STAT3 in SARS coronavirus-infected Vero E6 cells. FEBS Lett. 2004;577:187–192. doi: 10.1016/j.febslet.2004.10.005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Mizutani T, Fukushi S, Saijo M, Kurane I, Morikawa S. JNK and PI3K/Akt signaling pathways are required for establishing persistent SARS-CoV-infection in Vero E6 cells. Biochim. Biophys. Acta. 2005;1741:4–10. doi: 10.1016/j.bbadis.2005.04.004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Liao CL, Lin YL, Shen SC, Shen JY, Su HL, Huang YL, Ma SH, Sun YC, Chen KP, Chen LK. Antiapoptic but not antiviral function of human bcl-2 assists establishment of Japanese encephalitis virus persistence in cultured cells. J Virol. 1998;72:9844–9854. doi: 10.1128/jvi.72.12.9844-9854.1998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Appel E, Katzoff A, Ben-Moshe T, Kazimirsky G, Kobiler D, Lustig S, Brodie C. Differential regulation of Bcl-2 and Bax expression in cells infected with virulent and nonvirulent strains of sindbis virus. Virology. 2000;276:238–242. doi: 10.1006/viro.2000.0458. [DOI] [PubMed] [Google Scholar]
  • 11.Poruchynsky MS, Wang EE, Rudin CM, Blagosklonny MV, Fozo T. Bcl-xL is phosphorylated in malignant cells following microtubule disruption. Cancer Res. 1998;58:3331–3338. [PubMed] [Google Scholar]
  • 12.Kharbanda S, Saxena S, Yoshida K, et al. Translocation of SAPK/JNK to mitochondria and interaction with Bcl-x(L) in response to DNA damage. J. Biol. Chem. 2000;275:322–327. doi: 10.1074/jbc.275.1.322. [DOI] [PubMed] [Google Scholar]
  • 13.Basu A, Haldar S. Identification of a novel Bcl-xL phosphorylation site regulating the sensitivity of taxol- or 2-methoxyestradiol-induced apoptosis. FEBS Lett. 2003;538:41–47. doi: 10.1016/S0014-5793(03)00131-5. [DOI] [PubMed] [Google Scholar]
  • 14.Hinshaw VS, Olsen CW, Dybdahl-Sissoko N, Evans D. Apoptosis: a mechanism of cell killing by influenza A and B viruses. J. Virol. 1994;68:3667–3673. doi: 10.1128/jvi.68.6.3667-3673.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Ubol S, Tucker PC, Griffin DE, Hardwick JM. Neurovirulent strains of alphavirus induce apoptosis in bcl-2-expressing cells: role of a single amino acid change in the E2 glycoprotein. Proc. Natl. Acad. Sci. USA. 1994;91:5202–5206. doi: 10.1073/pnas.91.11.5202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Pekosz A, Phillips J, Pleasure D, Merry D, Gonzalez-Scarano F. Induction of apoptosis by La Crosse virus infection and role of neuronal differentiation and human bcl-2 expression in its prevention. J. Virol. 1996;70:5329–5335. doi: 10.1128/jvi.70.8.5329-5335.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]

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