Skip to main content
PMC home page
Springer Nature - PMC COVID-19 Collection logoLink to Springer Nature - PMC COVID-19 Collection
. 2006;581:609–613. doi: 10.1007/978-0-387-33012-9_111

Identification and Evaluation of Coronavirus Replicase Inhibitors Using a Replicon Cell Line

Elke Scandella 3, Klara K Eriksson 4, Tobias Hertzig 5, Christian Drosten 6, Lili Chen 7, Chunshan Gui 8, Xiaomin Luo 9, Jianhua Shen 10, Xu Shen 11, Stuart G Siddell 12, Burkhard Ludewig 13, Hualiang Jiang 14, Stephan Günther 15, Volker Thiel 16
Editors: Stanley Perlman1, Kathryn V Holmes2
PMCID: PMC7123120  PMID: 17037607

The content is available as a PDF (906.6 KB).

Contributor Information

Stanley Perlman, Email: Stanley-Perlman@uiowa.edu

Kathryn V. Holmes, Email: Kathryn.Holmes@ucHSC.edu

References

  • 1.Hertzig T, Scandella E, Schelle B, Ziebuhr J, Siddell SG, Ludewig B, Thiel V. Rapid identification of coronavirus replicase inhibitors using a selectable replicon RNA. J. Gen. Virol. 2004;85:1717–1725. doi: 10.1099/vir.0.80044-0. [DOI] [PubMed] [Google Scholar]
  • 2.Rubin B, Piala JJ, Burke JC, Craver BN. A new, potent and specific serotonin inhibitor (Sq 10,643) 2'-(3-dimethylaminopropylthio) cin-namanilide hydrochloride: antoserotonin activity on uterus and on gastrointestinal, vascular, and respiratory systems of animals. Arch. Int. Pharmacodyn. Ther. 1964;152:132–143. [PubMed] [Google Scholar]
  • 3.Gallant DM, Bishop MP. Cinanserin (SQ. 10,643): a preliminary evaluation in chronic schizophrenic patients. Curr. Ther. Res. Clin. Exp. . 1968;10:461–463. [PubMed] [Google Scholar]
  • 4.Itil TM, Polvan N, Holden JM. Clinical and electroencephalographic effects of cinanserin in schizophrenic and manic patients. Dis. Nerv. Syst. 1971;32:193–200. [PubMed] [Google Scholar]
  • 5.Chen L, Gui C, Luo X, et al. Cinanserin is an inhibitor of the 3C-like proteinase of severe acute respiratory syndrome coronavirus and strongly reduces virus replication in vitro. J. Virol. 2005;79:7095–7103. doi: 10.1128/JVI.79.11.7095-7103.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Thiel V, Herold J, Schelle B, Siddell SG. Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus. J. Gen. Virol. 2001;82:1273–1281. doi: 10.1099/0022-1317-82-6-1273. [DOI] [PubMed] [Google Scholar]
  • 7.Ziebuhr J, Snijder EJ, Gorbalenya AE. Virus-encoded proteinases and proteolytic processing in the Nidovirales. J. Gen. Virol. 2000;81:853–879. doi: 10.1099/0022-1317-81-4-853. [DOI] [PubMed] [Google Scholar]
  • 8.Anand K, Ziebuhr J, Wadhwani P, Mesters JR, Hilgenfeld R. Coronavirus main proteinase (3CLpro) structure: basis for design of anti-SARS drugs. Science. 2003;300:1763–1767. doi: 10.1126/science.1085658. [DOI] [PubMed] [Google Scholar]

Articles from The Nidoviruses are provided here courtesy of Nature Publishing Group

RESOURCES