Abstract
A compact model of imaging ellipsometer is used for the data acquisition and analysis of label free protein microarray. Its principle, methodology, and experimental setup as well as sampling conditions and a demonstration result are presented here. Furthermore, results of protein adsorption and protein interaction in the microarray may be deduced. This shows that the compact model is effectively performed as a reader for the protein microarray sampling and provides a potential for applications of label free protein microarrays on site of care.
Keywords: imaging ellipsometer, compact model, label free protein microarray
References
- 1.Jin G., Roger J., Arwin H. Imaging ellipsometry revisited: Developments for visualization of thin transparent layers on silicon substrates. Rev. Sci. Instrum. 1996;67:2930–2936. doi: 10.1063/1.1147074. [DOI] [Google Scholar]
- 2.Jin G., Wang Z.H. Micro-systems for optical protein-chip. Int. J. Nonlinear Sci. Number. Simul. 2002;3:191–194. [Google Scholar]
- 3.Jin G, Wang ZH, Meng YH. (1998) Imaging ellipsometry for the visualization of bio-molecular layers. in Proc. 20th Inernational Conference of the IEEE Engineering in Medicine and Biology Society. Hong Kong. IEEE, 581–584
- 4.Wang Z.H., Jin G. A lable-free multisensing immunosensor based on imaging ellipsometry. Anal Chem. 2003;75:6119–6123. doi: 10.1021/ac0347258. [DOI] [PubMed] [Google Scholar]
- 5.Jin G, Wang ZH, Meng YH et al. (2001) Optical proteinchip as microarrays for protein interaction determination. in Proc. 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Istanbul, Turkey, p. 58–61
- 6.Zhang H.G., Qi C., Wang Z.H., et al. Evaluation of a new CA15-3 protein assay method: optical protein-chip system for clinical application. Clinical Chemistry. 2005;51:1038–1040. doi: 10.1373/clinchem.2004.043240. [DOI] [PubMed] [Google Scholar]
- 7.Qi C., Duan J.Z., Wang Z.H. Investigation of interactions between two neutralizing monoclonal antibodies and SARS virus using biosensor based on imaging ellipsometry. Biomed Microdevices. 2006;8:186–192. doi: 10.1007/s10544-006-8305-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 8.Chen Y.-Y., Meng Y.-H., Jin G. Optimization of off-null ellipsometry for air/solid interface [J] Appl Opt. 2007;46:8475–8481. doi: 10.1364/AO.46.008475. [DOI] [PubMed] [Google Scholar]
- 9.Zhan-hui W., Yong-hong M., Pei-qing Y., Cai Q., Gang J. A label-free protein microfluidic array for immunoassays. Electrophoresis. 2006;27:4078–4085. doi: 10.1002/elps.200500956. [DOI] [PubMed] [Google Scholar]