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. 2006;581:313–318. doi: 10.1007/978-0-387-33012-9_55

Three-Dimensional Reconstruction of the Nucleolus Using Meta-Confocal Microscopy in Cells Expressing the Coronavirus Nucleoprotein

Jae-Hwan You 3, Mark L Reed 4, Brian K Dove 5, Julian A Hiscox 6
Editors: Stanley Perlman1, Kathryn V Holmes2
PMCID: PMC7124029  PMID: 17037551

The content is available as a PDF (1.4 MB).

Contributor Information

Stanley Perlman, Email: Stanley-Perlman@uiowa.edu

Kathryn V. Holmes, Email: Kathryn.Holmes@ucHSC.edu

References

  • 1.Hiscox JA. The interaction of animal cytoplasmic RNA viruses with the nucleus to facilitate replication. Virus Res. 2003;95:13–22. doi: 10.1016/S0168-1702(03)00160-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Hiscox JA. The nucleolus--a gateway to viral infection? Arch. Virol. 2002;147:1077–1089. doi: 10.1007/s00705-001-0792-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Rowland RR, Yoo D. Nucleolar-cytoplasmic shuttling of PRRSV nucleocapsid protein: a simple case of molecular mimicry or the complex regulation by nuclear import, nucleolar localization and nuclear export signal sequences. Virus Res. 2003;95:23–33. doi: 10.1016/S0168-1702(03)00161-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Leung AK, Andersen JS, Mann M, Lamond AI. Bioinformatic analysis of the nucleolus. Biochem. J. 2003;376:553–569. doi: 10.1042/BJ20031169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Chen H, Gill A, Dove BK, Emmett SR, Kemp CF, Ritchie MA, Dee M, Hiscox JA. Mass spectroscopic characterization of the coronavirus infectious bronchitis virus nucleoprotein and elucidation of the role of phosphorylation in RNA binding by using surface plasmon resonance. J. Virol. 2005;79:1164–1179. doi: 10.1128/JVI.79.2.1164-1179.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Calvo E, Escors D, Lopez JA, Gonzalez JA, Alvarez A, Arza E, Enjuanes L. Phosphorylation and subcellular localization of transmissible gastroenteritis virus nucleocapsid protein in infected cells. J. Gen. Virol. 2005;86:2255–2267. doi: 10.1099/vir.0.80975-0. [DOI] [PubMed] [Google Scholar]
  • 7.Parker MM, Masters PS. Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three domain structure for the nucleocapsid protein. Virology. 1990;179:463–468. doi: 10.1016/0042-6822(90)90316-J. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Lundberg M, Johansson M. Positively charged DNA-binding proteins cause apparent cell membrane translocation. Biochem. Biophys. Res. Commun. 2002;291:367–371. doi: 10.1006/bbrc.2002.6450. [DOI] [PubMed] [Google Scholar]
  • 9.Sheval EV, Polzikov MA, Olson MO, Zatsepina OV. A higher concentration of an antigen within the nucleolus may prevent its proper recognition by specific antibodies. Eur. J. Histochem. 2005;49:117–123. [PubMed] [Google Scholar]

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