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. 2003 Apr 5;11(4):423–433. doi: 10.1016/S0969-2126(03)00050-9

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Copyright © 2003 Cell Press. Published by Elsevier Ltd. All rights reserved.

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SDS Resistance of Oligomeric Ring-like VP40 in Complex with RNA

(A) SDS resistance of VP40(31–212). Lane 1, boiled sample; lane 2, nonboiled sample; lane 3, nonboiled sample of crystals of VP40(31–212) in space group P6₂22.

(B) SDS resistance of full-length VP40. Lane 1, full-length recombinant VP40 destabilized in the presence of E. coli nucleic acids and separated on SDS-PAGE; lane 1, nonboiled; lane 2, boiled. Lane 3, detection of full-length VP40 in Ebola virus infected cells under nonboiling conditions and boiled (lane 4). Note that the high molecular weight form in lane 3 corresponds to the size of the oligomeric full-length VP40 generated in vitro (lane 1). The monomeric form and the proposed octameric form are both indicated by arrows and a band corresponding to a dimer (lane 2) is indicated by an asterisk.

(C) SDS resistance analysis of VP40 present in purified Ebola virus particles. Samples were separated under boiled (lane 1) and nonboiled conditions (lane 2).

Samples were separated on gradient SDS-PAGE and positions of marker proteins are indicated. Bands in (A) were detected by Coomassie blue staining and in (B) and (C) by Western blot.