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. 2010 Dec 24;29(7):1481–1490. doi: 10.1016/j.vaccine.2010.12.028

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Copyright © 2010 Elsevier Ltd. All rights reserved.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 1 — Strategy for the construction of the recombinant AIK-C genome cDNAs having RSV protein genes. The recombinant AIK-C viral cDNAs expressing RSV G or F protein were constructed based on AIK-C cDNA (pMVAIK). pMVAIK/20-77 was constructed for the cloning of foreign genes. The Asc I site was introduced by adding GGCGCG after position 3432 of AIK-C and R1 and R2 sequences were added. The Nco I–Not I fragment of RSV G or F was cloned into pMVAIK/20-77, designed as pMVAIK/20-77/RSV. pMVAIK/20-77/RSV had unique restriction enzyme sites, Sac II and Pac I sites, located in the P gene and between the F and H gene. The DNA fragments between the Sac II and Pac I sites of pMVAIK/20-77/RSV/G and pMVAIK/20-77/RSV/F were inserted into pMVAIK using Sac II and Pac I sites. The recombinant plasmid constructs were designated pMVAIK/RSV/G and pMVAIK/RSV/F, respectively.