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. 2012 Sep 6;2(2):S855–S858. doi: 10.1016/S2221-1691(12)60323-2

Evaluation of antiviral and cytotoxic activities of methanolic extract of S. grandiflora (Fabaceae) flowers

Saravana Kumar Arthanari a,*, Jayachandran Vanitha b, Mani Ganesh c, Krishnasamy Venkateshwaran d, De Clercq e
PMCID: PMC7128231  PMID: 32289022

Abstract

Objective

To investigate the cytotoxicity and antiviral activity of methanolic extract of S. grandiflora flowers using different cell lines and viruses.

Methods

The methanolic flower extracts were prepared and evaluated for their antiviral and cytotoxic activities using viruses like herpes simplex-1 and 2, vaccinia, vesicular stomatitis, cox sackie, respiratory syncytical, feline corona, feline herpes, para influenza, reo-1, sindbis and punta toro viruses in different cell lines, like Hel, HeLa, Crandell Reus feline kidney and Vero cell cultures.

Results

Among the viruses used the extract possessed strongest antiviral activity against herpes simplex 1 and 2, repiratory syncytical, para influenza, reo, sindbis, cox sackie and punta toro viruses that was (EC50=20 μg/mL and 45 μg/mL) and moderate activity for remaining viruses (EC50= 100 μg/mL). The antiviral activities assessed by calculating the selectivity index may be due to the presence of flavonoids in the extracts there by inhibit the virus cell fusion in the early and replication stages. The cytotoxicity effect was evaluated using MTT assay and the results revealed that the extracts exhibited cytotoxicity from the range of 20 to 100 μg/mL.

Conclusions

Present results confirmed that the S. grandiflora used as a good antimicrobial agent in future.

Keywords: S. grandiflora, Antiviral, Cytotoxicity, Herpes simplex virus

Footnotes

Available online 28 August 2012

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