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. 2012 Apr 28;5(5):342–346. doi: 10.1016/S1995-7645(12)60055-8

Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus

Piyathida Pongsiri a, Kesmanee Praianantathavorn b, Apiradee Theamboonlers a, Sunchai Payungporn b, Yong Poovorawan a,*
PMCID: PMC7128799  PMID: 22546647

Abstract

Objective

To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection.

Methods

We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR.

Results

The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus.

Conclusions

This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.

Keywords: Multiplex, Real-time RT-PCR, Chikungunya virus, Dengue virus

Footnotes

Available online 20 May 2012

Foundation project: This study was supported by the Center of Excellence in Clinical Virology, Chulalongkorn University, CU Centenary Academic Development Project, King Chulalongkorn Memorial Hospital, the National Research University project of CHE and the Ratchadaphiseksonphot Endowment Fund(HR1155A).

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