Abstract
The prevalence and phylogeny of feline coronaviruses were studied in urban cat populations by sampling of 113 clinically healthy cats. Rectal swab samples were subjected to a nested reverse-transcription polymerase chain reaction, specific for the conservative nucleocapsid region of the virus genome. More than 30% of the sampled animals proved positive for the presence of feline coronaviruses. The nucleotide sequences of amplified 440 bp products were determined, aligned and the phylogenetic analysis revealed noticeable genetic clusters among the prevalent feline coronaviruses in the surveyed geographic area. These findings will hopefully contribute to the elucidation of the epidemiology of feline infectious peritonitis.
Keywords: Feline, coronavirus, RT-PCR, prevalence, phylogeny.
References
REFERENCES
- 1.ADDIE D.D., JARRETT J.O. A study of naturally occurring feline coronavirus infections in kittens. Veterinary Record. 1992;130:133–137. doi: 10.1136/vr.130.7.133. [DOI] [PubMed] [Google Scholar]
- 2.ADDIE D.D., JARRETT J.O. Feline coronavirus antibodies in cats. Veterinary Record. 1992;131:202–203. doi: 10.1136/vr.131.9.202-a. [DOI] [PubMed] [Google Scholar]
- 3.BARIC R.S., FU K.F., SCHAAD M.C., STOHLMAN S.A. Establishing a genetic recombination map of murine coronavirus strain A59 complementation groups. Virology. 1990;177:646–656. doi: 10.1016/0042-6822(90)90530-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4.BOOM R., SOL C.J.A., SALIMANS M.M.M., JANSEN C.L., WERTHEIM-VAN DI.L.L.E.N, P.M.E., VAN DE.R NO.O.R.E.D.A.A, J. Rapid and simple method for purification of nucleic acid. Journal of Clinical Microbiology. 1990;28:495–503. doi: 10.1128/jcm.28.3.495-503.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5.CHEUNG R.C., MATSUI S., GREENBERG H. Rapid and sensitive method for detection of hepatitis C virus RNA by using silica particles. Journal of Clinical Microbiology. 1994;32:2593–2597. doi: 10.1128/jcm.32.10.2593-2597.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6.DE GR.O.O.T, R.J., TER HA.A.R, R.J., HORZINEK M.C., VAN DE.R ZE.I.J.S.T, B.A. Intracellular RNAs of the feline infectious peritonitis virus strain 79–1146. Journal of General Virology. 1987;68:995–1002. doi: 10.1099/0022-1317-68-4-995. [DOI] [PubMed] [Google Scholar]
- 7.DE GR.O.O.T, R.J., HORZINEK M.C. Feline infectious peritonitis. In: Siddell, S.G, editors. The Coronaviridae. Plenum Press; New York: 1995. pp. 293–315. [Google Scholar]
- 8.DE VR.I.E.S, A.A.F., HORZINEK M.C., ROTTIER P.J.M., DE GR.O.O.T, R. The genome organization of the Nidovirales: similarities and differences between Arteri-, Toro-, and Coronaviruses. Seminars in Virology. 1997;8:33–47. doi: 10.1006/smvy.1997.0104. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.EVERMANN J.F., MCKEIRNAN A.J., OTT R.L. Perspectives on the epizootiology of feline enteric coronavirus and the pathogenesis of feline infectious peritonitis. Veterinary Microbiology. 1991;28:243–255. doi: 10.1016/0378-1135(91)90079-U. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10.FEHR D., HOLZNAGEL E., BOLLA S., HAUSER B., HERREWEGH A.A., HORZINEK M.C., LUTZ H. Placebo-controlled evaluation of a modified life virus vaccine against feline infectious peritonitis: safety and efficacy under field conditions. Vaccine. 1997;15:1101–1109. doi: 10.1016/S0264-410X(97)00006-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.FOLEY J.F., PEDERSEN N.C. The inheritance of susceptibility to feline infectious peritonitis in purebred catteries. Feline Practice. 1996;24:14–22. [Google Scholar]
- 12.FOLEY J.F., POLAND A., CARLSON J., PEDERSEN N.C. Risk factors for feline infectious peritonitis among cats in multiple-cat environments with endemic feline enteric coronavirus. Journal of American Veterinary Medical Association. 1997;210:1313–1318. [PubMed] [Google Scholar]
- 13.FOLEY J.F., POLAND A., CARLSON J., PEDERSEN N.C. Patterns of feline coronavirus infection and fecal shedding from cats in multiple-cat environments. Journal of American Veterinary Medical Association. 1997;210:1307–1312. [PubMed] [Google Scholar]
- 14.GAMBLE D.A., LOBBIANI A., GRAMEGNA M., MOORE L.E., COLUCCI G. Development of a nested PCR assay for detection of feline infectious peritonitis virus in clinical specimens. Journal of Clinical Microbiology. 1997;35:673–675. doi: 10.1128/jcm.35.3.673-675.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15.GUNN-MOORE D.A., GRUFFYDD-JONES T.J., HARBOUR D.A. Detection of feline coronaviruses by culture and reverse transcriptase-polymerase chain reaction of blood samples from healthy cats and cats with clinical feline infectious peritonitis. Veterinary Microbiology. 1998;62:193–205. doi: 10.1016/S0378-1135(98)00210-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 16.HARVEY C.J., LOPEZ J.W., HENDRICK M.J. An uncommon intestinal manifestation of feline infectious peritonitis: 26 cases (1986–1993) Journal of American Veterinary Medical Association. 1996;209:1117–1120. [PubMed] [Google Scholar]
- 17.HERREWEGH A.A.P.M., DE GR.O.O.T, R., CEPICA A., EGBERINK H.F., HORZINEK M.C., ROTTIER P.J.M. Detection of feline coronavirus RNA in feces, tissues, and body fluids of naturally infected cats by reverse transcriptase PCR. Journal of Clinical Microbiology. 1995;33:684–689. doi: 10.1128/jcm.33.3.684-689.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 18.HERREWEGH A.A.P.M., MÄHLER M., HEDRICH H.J., HAAGMANS B.L., EGBERINK H.F., HORZINEK M.C., ROTTIER P.J.M., DE GR.O.O.T, R. Persistence and evolution of feline coronavirus in a closed cat-breeding colony. Virology. 1997;234:349–363. doi: 10.1006/viro.1997.8663. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 19.HERREWEGH A.A.P.M., SMEENK I., HORZINEK M.C., ROTTIER P.J.M., DE GR.O.O.T, R. Feline coronavirus type II strains 79–1683 and 79–1146 originate from a double recombination between feline coronavirus type I and canine coronavirus. Journal of Virology. 1998;72:4508–4514. doi: 10.1128/jvi.72.5.4508-4514.1998. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 20.HIGGINS D.G., BLEASBY A.J., FUCHS R. Clustal V: Improved software for multiple sequence alignment. CABIOS. 1992;8:189–191. doi: 10.1093/bioinformatics/8.2.189. [DOI] [PubMed] [Google Scholar]
- 21.HOHDATSU T., OKADA S., KOYAMA H. Characterization of monoclonal antibodies against feline infectious peritonitis virus type II and antigenic relationship between feline, porcine, and canine coronaviruses. Archives of Virology. 1991;117:85–95. doi: 10.1007/BF01310494. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 22.HOHDATSU T., SASAMOTO T., OKADA S., KOYAMA H. Antigenic analysis of feline coronaviruses with monoclonal antibodies (Mabs): preparation of Mabs which discriminate between FIPV strain 79–1146 and FECV strain 79–1683. Veterinary Microbiology. 1991;28:13–24. doi: 10.1016/0378-1135(91)90096-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 23.HOHDATSU T., OKADA S., ISHIZUKA Y., YAMADA H., KOYAMA H. The prevalence of types I and II feline coronavirus infections in cats. Journal of Veterinary Medical Science. 1992;54:557–562. doi: 10.1292/jvms.54.557. [DOI] [PubMed] [Google Scholar]
- 24.HORSBURGH B.C., BRIERLEY I., BROWN T.D. Analysis of a 9.6 kb sequence from the 3′ end of canine coronavirus genomic RNA. Journal of General Virology. 1992;73:2849–2862. doi: 10.1099/0022-1317-73-11-2849. [DOI] [PubMed] [Google Scholar]
- 25.KECK J.G., MATSUSHIMA G.K., MAKINO S., FLEMING S., VANNIER D.M., STOHLMAN S.A., LAI M.M.C. In vivo RNA-RNA recombination of coronavirus in mouse brain. Journal of Virology. 1988;62:1810–1813. doi: 10.1128/jvi.62.5.1810-1813.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 26.KOTTIER S.A., CAVANAGH D., BRITTON P. Experimental evidence of recombination in coronavirus infectious bronchitis virus. Virology. 1995;213:569–580. doi: 10.1006/viro.1995.0029. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 27.KUSTERS J.G., JAGER E.J., NIESTERS B.G.M., ZEIST B.A.M. Sequence evidence for RNA recombination in fireld isolates of avian coronavirus infectious bronchitis virus. Vaccine. 1990;8:605–608. doi: 10.1016/0264-410X(90)90018-H. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 28.LAI M.M.C., BARIC R.C., MAKINO S., KECK J.G., ENGBERT J., LEIBOWITZ J.L., STOHLMAN S.A. Recombination between nonsegmented RNA genomes of murine coronaviruses. Journal of Virology. 1985;56:449–456. doi: 10.1128/jvi.56.2.449-456.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 29.LAI M.M.C. Recombination in large RNA viruses: coronaviruses. Seminars in Virology. 1996;7:381–388. doi: 10.1006/smvy.1996.0046. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 30.LI X., SCOTT F.W. Detection of feline coronaviruses in cell cultures and in fresh and fixed feline tissues using polymerase chain reaction. Veterinary Microbiology. 1994;42:65–77. doi: 10.1016/0378-1135(94)90078-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 31.LUYTJES W. Coronavirus gene expression: genome organization and protein expression. In: Siddell S.G., editor. The Coronaviridae. Plenum Press; New York: 1995. pp. 33–49. [Google Scholar]
- 32.MAKINO S., KECK J.G., STOHLMAN S.A., LAI M.M.C. High-frequency RNA recombination of murine coronaviruses. Journal of Virology. 1986;57:729–739. doi: 10.1128/jvi.57.3.729-737.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 33.MOTOKAWA K., HOHDATSU T., HASHIMOTO H., KOYAMA H. Comparison of the amino acid sequence and phylogenetic analysis of the peplomer, integral membrane and nucleocapsid proteins of feline, canine and porcine coronaviruses. Microbiology and Immunology. 1996;40:425–433. doi: 10.1111/j.1348-0421.1996.tb01089.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 34.OLSEN C.W. A review of feline infectious peritonitis virus: molecular biology, immunopathogenesis, clinical aspects, and vaccination. Veterinary Microbiology. 1993;36:1–37. doi: 10.1016/0378-1135(93)90126-R. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 35.PALTRINIERI S., PARODI CA.M.M.A.R.A.T.A, M., CAMMARATA G., COMAZZI S. Some aspects of humoral and cellular immunity in naturally occuring feline infectious peritonitis. Veterinary Immunology and Immunpathology. 1998;65:205–220. doi: 10.1016/S0165-2427(98)00155-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 36.PEDERSEN N.C. Serologic studies of naturally occurring feline infectious peritonitis. American Journal of Veterinary Research. 1976;37:1449–1453. [PubMed] [Google Scholar]
- 37.PEDERSEN N.C. Virologic and immunologic aspects of feline infectious peritonitis virus infection. Advances in Experimental Medicine and Biology. 1987;218:529–550. doi: 10.1007/978-1-4684-1280-2_69. [DOI] [PubMed] [Google Scholar]
- 38.PEDERSEN N.C. An overview of feline enteric coronavirus and infectious peritonitis virus infections. Feline Practice. 1987;23:7–22. [Google Scholar]
- 39.POLAND A.M., VENNEMA H., FOLEY J.E., PEDERSEN N.C. Two related strains of feline infectious peritonitis virus isolated from immunocompromised cats infected with a feline enteric coronavirus. Journal of Clinical Microbiology. 1996;34:3180–3184. doi: 10.1128/jcm.34.12.3180-3184.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 40.SIDDELL S., WEGE H., TER ME.U.L.E.N, V. The biology of coronaviruses. Journal of General Virology. 1983;64:761–776. doi: 10.1099/0022-1317-64-4-761. [DOI] [PubMed] [Google Scholar]
- 41.VENNEMA H., DE GR.O.O.T, R., HARBOUR D.A., DALDERUP M., GRUFFYDD-JONES T., HORZINEK M.C., SPAAN W.J.M. Early death after feline infectious peritonitis virus challenge due to recombinant vaccinia virus immunization. Journal of Virology. 1990;64:1407–1409. doi: 10.1128/jvi.64.3.1407-1409.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 42.VENNEMA H., DE GR.O.O.T, R.J., HARBOUR D.A., HORZINEK M.C., SPAAN W.J. Primary structure of the membrane and nucleocapsid protein genes of feline infectious peritonitis virus and immunogenicity of recombinant vaccinia viruses in kittens. Virology. 1991;181:327–335. doi: 10.1016/0042-6822(91)90499-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 43.VENNEMA H., POLAND A., FOLEY J., PEDERSEN N.C. Feline infectious peritonitis viruses arise by mutation from endemic feline enteric coronaviruses. Virology. 1998;243:150–157. doi: 10.1006/viro.1998.9045. [DOI] [PMC free article] [PubMed] [Google Scholar]