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. 2005 Mar 3;153(3):287–305. doi: 10.1016/S1090-0233(97)80063-9

Detection of animal pathogens by using the polymerasechain reaction (PCR)

JM Rodriguez 1
PMCID: PMC7129519  PMID: 9232118

Abstract

The polymerase chain reaction (PCR) is a nucleic acid-based technique that enables the rapid and sensitive detection of specific micro-organisms. Although this technique is widely used in veterinary research, it has not yet found applications in routine microbiological analysis of veterinary clinical samples. However, advances in sample preparation together with the increasing availability of specific gene sequences will probably lead to the more widespread diagnostic use of PCR in the future. PCR is likely to have a strong impact in the epidemiology, treatment and prevention of animal infectious diseases.

Keywords: PCR, diagnosis, virus, bacteria, parasites

References

  1. Aabo S., Rasmussen O.F., Rossen L., Sorensen P.D., Olsen J.E. Salmonella identification by the polymerase chain reaction. Molecular and Cellular Probes. 1993;7:171–178. doi: 10.1006/mcpr.1993.1026. [DOI] [PubMed] [Google Scholar]
  2. Agresti A., Ponti W., Rocchi M., Meneveri R., Marozzi A., Cavalleri D., Peri E., Poli G., Ginelli E. Use of polymerase chain reaction to diagnose bovine leukemia-virus infection in calves at birth. American Journal of Veterinary Research. 1993;54:373–378. [PubMed] [Google Scholar]
  3. Akita G.Y., Glenn J., Castro A.E., Osburn B.I. Detection of bluetongue virus in clinical samples by polymerase chain reaction. Journal of Veterinary Diagnostic Investigation. 1993;5:154–158. doi: 10.1177/104063879300500202. [DOI] [PubMed] [Google Scholar]
  4. Aly M.M., Smith E.J., Fadly A.M. Detection of reticuloendotheliosis virus infection using the polymerase chain reaction. Avian Pathology. 1993;22:543–554. doi: 10.1080/03079459308418942. [DOI] [PubMed] [Google Scholar]
  5. Andreasen J.R., Jackwood M.W., Hilt D.A. Polymerase chain reaction amplification of the genome of infectious bronchitis virus. Avian Diseases. 1991;35:216–220. [PubMed] [Google Scholar]
  6. Aradaib I.E., Akita G.Y., Pearson J.E., Osburn B.I. Comparison of polymerase chain reaction and virus isolation for detection of epizootic hemorrhagic disease in clinical samples from naturally infected deer. Journal of Veterinary Diagnostic Investigation. 1993;7:196–200. doi: 10.1177/104063879500700205. [DOI] [PubMed] [Google Scholar]
  7. Ballagy-Pordany A., Klingeborn B., Flensburg J., Belak S. Equine herpesvirus type 1: detection of viral DNA sequences in aborted fetuses with the polymerase chain reaction. Veterinary Microbiology. 1990;22:373–381. doi: 10.1016/0378-1135(90)90024-p. [DOI] [PubMed] [Google Scholar]
  8. Ballagy-Pordany A., Klintevall K., Merza M., Klingeborn G., Belak S. Direct detection of bovine leukemia virus infection: practical applicability of the polymerase chain reaction. Journal of Veterinary Medicine B. 1992;39:69–77. doi: 10.1111/j.1439-0450.1992.tb01140.x. [DOI] [PubMed] [Google Scholar]
  9. Bandi C., La Rosa G., Comincini S., Damiani G., Pozio E. Random amplified polymorphic DNA technique for the identifacation of Trichinella species. Parasitology. 1993;107:419–424. doi: 10.1017/s0031182000067779. [DOI] [PubMed] [Google Scholar]
  10. Barrett T., Crowther J., Osterhaus A.D.M.E., Subbarao S.M., Groen J., Haas L., Mamaev L.V., Titenko A.M., Grachev M.A., Visser I.K.G., Bostock C.J. Molecular and serological studies on the recent seal virus epizooties in Europe and Siberia. Science of the Total Environment. 1992;115:117–132. doi: 10.1016/0048-9697(92)90037-s. [DOI] [PubMed] [Google Scholar]
  11. Barrett T., Amarel-Doel C., Kitching R.P., Gusev A. Use of polymerase chain reaction in differentiating rinderpest field virus and vaccine virus in the same animals. Revue Scientifique et Technique. Office International des Epizooties. 1993;12:865–872. doi: 10.20506/rst.12.3.734. [DOI] [PubMed] [Google Scholar]
  12. Bashiruddin J.B., Nicholas R.A.J., Santin F.G., Ready R.A., Woodward M.J., Taylor T.K. Use of polymerase chain reaction to detect mycoplasma DNA in cattle with contagious bovine pleuropneumonia. Veterinary Record. 1994;134:240–241. doi: 10.1136/vr.134.10.240. [DOI] [PubMed] [Google Scholar]
  13. Becker Y., Asher Y., Tabor E., Davidson I., Malkinson M., Weisman Y. Polymerase chain reaction for differentiation between pathogenic and non-pathogenetic serotype 1 Marek's disease virus (MDV) and vaccine viruses ofMDV-serotypes 2 and 3. Journal of Virological Methods. 1992;40:307–322. doi: 10.1016/0166-0934(92)90089-v. [DOI] [PubMed] [Google Scholar]
  14. Becker Y., Tabor E., Asher Y., Davidson I., Malkinson M., Witter R.L. PCR detection of amplified 132 bp repeats in Marek's disease virus type 1 (MDV-1) DNA can serve as an indicator of initial genomic rearrangement leading to the attenuation of virus virulence. Virus Genes. 1993;7:277–287. doi: 10.1007/BF01702588. [DOI] [PubMed] [Google Scholar]
  15. Belak S., Ballagy-Pordany A. Bovine viral diarrhea virus infection: rapid diagnosis by the polymerase chain reaction. Archives of Virology. 1991;3:181–190. doi: 10.1007/978-3-7091-9153-8_22. (Suppl.) [DOI] [PubMed] [Google Scholar]
  16. Belak S., Ballagy-Pordany A., Flensburg A., Virtanen J. Detection of pseudorabies virus DNA sequences by the polymerase chain reaction. Archives of Virology. 1989;108:279–286. doi: 10.1007/BF01310940. [DOI] [PubMed] [Google Scholar]
  17. Boye M., Kamstrup S., Dalsgaard K. Specific sequence amplification of bovine viral diarrhea virus (BVDV) and hog cholera virus and sequencing of BVDV nucleic acid. Veterinary Microbiology. 1991;29:1–13. doi: 10.1016/0378-1135(91)90106-p. [DOI] [PubMed] [Google Scholar]
  18. Brock K.V. Detection of persistent bovine viral diarrhea virus infections by DNA hybridization and polymerase chain reactions assay. Archives of Virology. 1991;(Suppl. 3):199–208. doi: 10.1007/978-3-7091-9153-8_24. [DOI] [PubMed] [Google Scholar]
  19. Brockmeier S.L., Lager K.M., Mengeling W.L. Comparison of in vitro reactivation and polymerase chain reaction for detection of latent pseudorabies virus infection in swine. Journal of Veterinary Diagnostic Investigation. 1993;5:505–509. doi: 10.1177/104063879300500401. [DOI] [PubMed] [Google Scholar]
  20. Buck G.E., O'Hara L.C., Summersgill J.T. Rapid, simple method for treating clinical samples containing Mycobacterium tuberculosis to remove DNA for polymerase chain reaction. Journal of Clinical Microbiology. 1992;30:1331–1334. doi: 10.1128/jcm.30.5.1331-1334.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Buogo C., Capaul S., Hani H., Frey J., Nicolet J. Diagnosis of Clostridium perfringens type C enteritis in pigs using a DNA amplification technique (PCR) Journal of Veterinary Medicine B. 1995;42:51–58. doi: 10.1111/j.1439-0450.1995.tb00681.x. [DOI] [PubMed] [Google Scholar]
  22. Burnens A.P., Frey A., Lior H., Nicolet J. Prevalence and clinical significance of vero-cytotoxin-producing Escherichia coli (VTEC) isolates from cattle in herds with and without calf diarrhea. Journal of Veterinary Medicine B. 1995;42:311–318. doi: 10.1111/j.1439-0450.1995.tb00715.x. [DOI] [PubMed] [Google Scholar]
  23. Carl M., Hawkins S.R., Coulson N., Lowe J., Robertson D.L., Nelson W.M., Titball R.W., Woody J.N. Detection of spores of Bacillus anthracis using the polymerase chain reaction. Journal of Infectious Diseases. 1992;165:1145–1148. doi: 10.1093/infdis/165.6.1145. [DOI] [PubMed] [Google Scholar]
  24. Chamberlain R.W., Wamwayi H.M., Hockley E., Shaila M.S., Goatley L., Knowlesn J.C., Barrett T. Evidence for different lineages of rinderpest virus reflecting their geographic isolation. Journal of General Virology. 1993;74:2775–2780. doi: 10.1099/0022-1317-74-12-2775. [DOI] [PubMed] [Google Scholar]
  25. Cheung A.K. Investigation of pseudorabies virus DNA and RNA in trigeminal ganglia and tonsil tissues of latently infected swine. American Journal of Veterinary Research. 1995;56:45–50. [PubMed] [Google Scholar]
  26. Chirnside E.D., Spaan W.J.M. Reverse transcription and cDNA amplification by the polymerase chain reaction of equine arteritis virus (EAV) Journal of Virological Methods. 1990;30:133–140. doi: 10.1016/0166-0934(90)90014-7. [DOI] [PubMed] [Google Scholar]
  27. Clavijo A., Thorsen J. Chemiluminescent detection of caprine arthritis encephalitis virus with a PCR-generated single stranded nonradiolabelled probe. Veterinary Microbiology. 1995;43:295–305. doi: 10.1016/0378-1135(94)00104-5. [DOI] [PubMed] [Google Scholar]
  28. Cohen N.D., McGruder E.D., Neibergs H.L., Behle R.W., Wallis D.E., Hargis B.M. Detection of Salmonella enteritidis in feces from poultry using booster polymerase chain reaction and oligonucleotide primers specific for all members of the genus Salmonella. Poultry Science. 1994;73:354–357. doi: 10.3382/ps.0730354. [DOI] [PubMed] [Google Scholar]
  29. Cohen N.D., Neibergs H.L., Wallis D.E., Simpson R.B., McGruder E.D. Genus-specific detection of salmonellae in equine feces by use of the polymerase chain reaction. American Journal of Veterinary Research. 1994;55:1049–1054. [PubMed] [Google Scholar]
  30. Cohen N.D., Wallis D.E., Neibergs H.L., McElroy A., McGruder E.D., De Loach J.R., Corrier D.E., Hargas B.M. Comparison of the polymerase chain reaction using genus-specific oligonucleotide primers and microbiologic cultures for the detection of Salmonella in drag-swabs from poultry houses. Poultry Science. 1994;73:1276–1281. doi: 10.3382/ps.0731276. [DOI] [PubMed] [Google Scholar]
  31. Cohen N.D., Wallis D.E., Neibergs H.L., Hargis B.M. Detection of Salmonella enteritidis in equine feces using the polymerase chain reaction and genus-specific oligonucleotide primers. Journal of Veterinary Diagnostic Investigation. 1995;7:219–222. doi: 10.1177/104063879500700209. [DOI] [PubMed] [Google Scholar]
  32. Cousins D.V., Wilton S.D., Francis B.R. Use of DNA amplification for the rapid identification of Mycobacterium bovis. Veterinary Microbiology. 1991;27:187–195. doi: 10.1016/0378-1135(91)90010-d. [DOI] [PubMed] [Google Scholar]
  33. Dangler C.A., Demattos C.A., Demattos C.C., Osbury B.I. Identifying bluetongue virus ribonucleic acid sequences by the polymerase chain reaction. Journal of Virological Methods. 1990;28:281–292. doi: 10.1016/0166-0934(90)90121-u. [DOI] [PubMed] [Google Scholar]
  34. Davidson I., Borovskaya A., Peri S., Malkinson M. Use of polymerase chain reaction for the diagnosis of natural infection of chickens and turkey with Marek's disease virus and reticuloendotheliosisvirus. Avian Pathology. 1995;24:69–94. doi: 10.1080/03079459508419050. [DOI] [PubMed] [Google Scholar]
  35. Denamur E., Sayada C., Souriau A., Orfila J., Rodolakis A., Elion J. Restriction pattern of the major outer membrane gene provides evidence for a homogeneous invasive group among ruminant isolates of Chlamydiapsittaci. Journal of Clinical Microbiology. 1991;137:2525–2530. doi: 10.1099/00221287-137-11-2525. [DOI] [PubMed] [Google Scholar]
  36. Diallo A., Libeau G., Couacy-Hymann E., Barbron M. Recent developments in the diagnosis of rinderpest and peste des ruminants. Veterinary Microbiology. 1995;44:307–317. doi: 10.1016/0378-1135(95)00025-6. [DOI] [PubMed] [Google Scholar]
  37. Dick T.A., Lu M., De Vos T., Ma K. The use of polymerase chain reaction to identify porcine isolates of Trichinella. Journal of Parasitology. 1992;78:145–148. [PubMed] [Google Scholar]
  38. Dupouy-Camet J., Soule C., Guillou J.P., Rover E., De Souza S.L., Ancelle T., Benarous R. Detection of repetitive sequences of Trichell spiralis by the polymerase chain reaction in experimentally infected mice. Parasitology Research. 1991;77:180–182. doi: 10.1007/BF00935434. [DOI] [PubMed] [Google Scholar]
  39. Dupouy-Camet J., Robert F., Soule C. RAPD in the genus Trichinella. Parasitology Today. 1993;9:463–464. doi: 10.1016/0169-4758(93)90101-k. [DOI] [PubMed] [Google Scholar]
  40. Egberink H.F., Herrewegh A.P.M., Schuurman N.P.M., Van Der Linde-Sipman J.S., Horzinek M.C., De Groot R.J. FIP, easy to diagnose? The Veterinary Quarterly. 1995;17:S24–S25. [PubMed] [Google Scholar]
  41. Epe C., Bienioschek S., Rehbein S., Schnieder T. Comparative RAPD-PCR analysis of lung-worms (Dictyocaulidae) from fallow deer, cattle, sheep and horses. Journal of Veterinary Medicine B. 1995;42:187–191. doi: 10.1111/j.1439-0450.1995.tb00699.x. [DOI] [PubMed] [Google Scholar]
  42. Ericsson H., Stalhandske P., Danielsson-Tham M.L., Bannerman E., Bille J., Jacquet C., Rocourt J., Tham W. Division of Listeria monocytogenes serovar 4b strains into two groups by PCR and restriction enzyme analysis. Applied and Environmental Microbiology. 1995;61:3872–3874. doi: 10.1128/aem.61.11.3872-3874.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Erlich H. Stockton Press; Providence: R.I.: 1989. PCR Technology: Principles and Applications for DNA Amplification. [Google Scholar]
  44. Ermine A., Larzul D., Ceccaldi P.E., Guesdon J.L., Tsiang H. Polymerase chain reaction amplification of rabies virus nucleic acids from total mouse brain RNA. Molecular and Cellular Probes. 1990;4:189–191. doi: 10.1016/0890-8508(90)90052-2. [DOI] [PubMed] [Google Scholar]
  45. Fach P., Delbart M.O., Schlachter A., Poumeyrol M., Popoff M.R. Apport de la technique d'amplification génique (PCR) au diagnostic des toxi-infections alimentaires a Clostridium perfringens. Médicine du Maladies Infectioses. 1993;23:70–77. [Google Scholar]
  46. Fach P., Gilbert M., Griffais R., Guillou J.P., Popoff M.R. PCR and gene probe identification of botulinum neurotoxin A-, B-, E-, F-, and G-producing Clostridium spp. and evaluation in food samples. Applied and Environmental Microbiology. 1995;61:389–392. doi: 10.1128/aem.61.1.389-392.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Fekete A., Bantle J.A., Halling S., Sanborn M.R. Preliminary development of a diagnostic test for Brucella using polymerase chain reaction. Journal of Applied Bacteriology. 1990;69:216–227. doi: 10.1111/j.1365-2672.1990.tb01512.x. [DOI] [PubMed] [Google Scholar]
  48. Fekete A., Bantle J.A., Halling S., Sanborn M.R. Rapid, sensitive detection of Brucella abortus by polymerase chain reaction without extraction of DNA. Biotechnology Techniques. 1990;4:31–34. [Google Scholar]
  49. Gerritsen M.J., Olyhoek T., Smits M.A., Bokhout B.A. Sample preparation method from polymerase chain reaction-based semiquantitative detection of Leptospira interrogans serovar hardjo serotypeHardjobovis in bovine urine. Journal of Clinical Microbiology. 1991;29:2805–2808. doi: 10.1128/jcm.29.12.2805-2808.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  50. Gonzalez L., Reid H.W., Pow I., Gilmour J.S. A disease resembling louping-ill in sheep in the Basque region of Spain. Veterinary Record. 1987;121:12–13. doi: 10.1136/vr.121.1.12. [DOI] [PubMed] [Google Scholar]
  51. Gottstein B. Molecular and immunological diagnosis of echinococcosis. Clinical Microbiology Review. 1992;5:248–261. doi: 10.1128/cmr.5.3.248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  52. Gottstein B., Mowatt M.R. Sequencing and characterization of an Echinococcus multilocularis DNA probe and its use in the polymerase chain reaction (PCR) Molecular and Biochemical Parasitology. 1991;44:183–194. doi: 10.1016/0166-6851(91)90004-p. [DOI] [PubMed] [Google Scholar]
  53. Gould A.R., Hyatt A.D., Eaton B.T., White J.R., Hooper P.T., Blacksell S.D., Leblanc Smith P.M. Current techniques in rapid bluetongue diagnosis. Australian Veterinary Journal. 1989;66:450–454. doi: 10.1111/j.1751-0813.1989.tb13575.x. [DOI] [PubMed] [Google Scholar]
  54. Gradil C.M., Harding M.J., Lewis K. Use of polymerase chain reaction to detect porcine parvovirus associated with swine embryos. American Journal of Veterinary Research. 1994;55:344–347. [PubMed] [Google Scholar]
  55. Gravekamp C., Van De Kemp H., Franzen M., Carrington D., Schoone G.J., Vaneys G.J.J.M., Everard C.O.R., Hartskeerl R.A., Terpstra W.J. Detection of seven species of pathogenic leptospires by PCR using two sets of primers. Journal of General Microbiology. 1993;139:1691–1700. doi: 10.1099/00221287-139-8-1691. [DOI] [PubMed] [Google Scholar]
  56. Griffin H.G., Griffin A.M. CRC Press; New York: 1994. PCR Technology. Current Innovations. [Google Scholar]
  57. Gruber A.D., Moenning V., Hewicker-Trautwein M., Trautwein G. Effect of formalin fixation and long-term storage on the detectability of bovine viral diarrhea virus (BVDV) RNA in archival brain tissue usingpolymerase chain reaction. Journal of Veterinary Medicine B. 1994;41:654–661. doi: 10.1111/j.1439-0450.1994.tb00276.x. [DOI] [PubMed] [Google Scholar]
  58. Guerin C., Allieta M., Guerin B., Thibier M. Utilisation de la technique d'amplification de gène pour la détection du virus de la maladie d'Aujeszky dans le sperme de verrat. Veterinary Research. 1995;26:140–144. [PubMed] [Google Scholar]
  59. Guevara P., Alonso G., Da Silveira J.F., De Mello M., Scorza J.V., Anez N., Ramirez J.L. Identification of a new world Leishmania using ribosomal gene spacer probes. Molecular and Biochemical Parasitology. 1992;56:15–26. doi: 10.1016/0166-6851(92)90150-i. [DOI] [PubMed] [Google Scholar]
  60. Harel J., Lapointe H., Fallara A., Lortie L.A., Bigras-Poulin M., Lariviere S., Fairbrother J.M. Detection of genes for fimbrial antigens and enterotoxins associated with Escherichia coli serogroups isolated from pigs with diarrhea. Journal of Clinical Microbiology. 1991;29:745–752. doi: 10.1128/jcm.29.4.745-752.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  61. Henderson I., Duggleby C.J., Turnbull P.C.B. Differentiation of Bacillus anthracis from other Bacillus cereus group bacteria with the PCR. International Journal of Systematic Bacteriology. 1994;44:99–105. doi: 10.1099/00207713-44-1-99. [DOI] [PubMed] [Google Scholar]
  62. Hertig C., Pauli V., Zanoni R., Peterhans E. Detection of bovine viral diarrhea (BVD) virus using the polymerase chain reaction. Veterinary Microbiology. 1991;26:65–76. doi: 10.1016/0378-1135(91)90042-e. [DOI] [PubMed] [Google Scholar]
  63. Hill W.E. The polymerase chain reaction: applications for the detection of foodborne pathogens. Critical Reviews in Food Science and Nutrition. 1996;36:123–173. doi: 10.1080/10408399609527721. [DOI] [PubMed] [Google Scholar]
  64. Hirasawa T., Kaneshige T., Mikazuki K. Sensitive detection of canine parvovirus DNA by the nested polymerase chain reaction. Veterinary Microbiology. 1994;41:135–145. doi: 10.1016/0378-1135(94)90143-0. [DOI] [PubMed] [Google Scholar]
  65. Hirasawa T., Yono K., Mikazuki K. Differentiation of wild- and vaccine-type canine parvoviruses by PCR and restriction-enzyme analysis. Journal of Veterinary Medicine B. 1995;42:601–610. doi: 10.1111/j.1439-0450.1995.tb00754.x. [DOI] [PubMed] [Google Scholar]
  66. Hoft Van Iodekinge B.J.L., Van Wamel J.L.V.B., Van Gennip H.G.P., Moorman R.J.M. Application of polymerase chain reaction to the detection of bovine viral diarrhea virus infections in cattle. Veterinary Microbiology. 1992;30:21–34. doi: 10.1016/0378-1135(92)90091-7. [DOI] [PubMed] [Google Scholar]
  67. Hookey J.V. Detection of Leptospiraceae by amplification of 16S ribosomal DNA. FEMS Microbiological Letters. 1992;90:267–274. doi: 10.1016/0378-1097(92)90659-c. [DOI] [PubMed] [Google Scholar]
  68. Horner G.W., Tham K.M., Orr D., Ralston J., Rowe S., Houghton T. Comparison of an antigen capture enzyme-linked assay with reverse transcription-polymerase chain reaction and cell culture immunoperoxidase tests forthe diagnosis of ruminant pestivirus infections. Veterinary Microbiology. 1995;43:75–84. doi: 10.1016/0378-1135(94)00080-g. [DOI] [PubMed] [Google Scholar]
  69. Horserace Betting Levy Board The Horserace Betting Levy Board's code of practice for equine viral arteritis for the 1994 breeding season. Veterinary Record. 1993;134:512–514. doi: 10.1136/vr.133.21.512. [DOI] [PubMed] [Google Scholar]
  70. Hutson R.A., Duggleby C.J., Lowe J.R., Manchee R.J., Turnbull P.C.B. The development and assessment of DNA and oligonucleotides for the specific detection of Bacillus anthracis. Journal of Applied Bacteriology. 1993;75:463–472. doi: 10.1111/j.1365-2672.1993.tb02803.x. [DOI] [PubMed] [Google Scholar]
  71. Hyman J.A., Johnson L.K., Tsai M.M., O'Leary T.J. Specificity of polymerase chain reaction identification of Toxoplasma gondii in paraffin-embedded animal tissues. Journal of Veterinary Diagnostic Investigation. 1995;7:275–278. doi: 10.1177/104063879500700223. [DOI] [PubMed] [Google Scholar]
  72. Innis M.A., Gelfand D.H., Sninsky J.J., White T.J. Academic Press; Boca Raton: 1990. PCR Protocols: A Guide to Methods and Applications. [Google Scholar]
  73. Jackwood M.W., Kwon H.M., Hilt D.A. Infectious bronchitis virus detection in allantoic fluid using the polymerase chain reaction and DNA probes. Avian Diseases. 1992;36:403–409. [PubMed] [Google Scholar]
  74. Jaton K., Sahli R., Bille J. Development of polymerase chain reaction assays for detection of Listeria monocytogenes in clinical cerebrospinal fluid samples. Journal of Clinical Microbiology. 1992;30:1931–1936. doi: 10.1128/jcm.30.8.1931-1936.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  75. Jestin V., Jestin A. Detection of Newcastle disease virus RNA in infected allantoic fluids by in vitro enzymatic amplification (PCR) Archives of Virology. 1991;118:151–161. doi: 10.1007/BF01314026. [DOI] [PubMed] [Google Scholar]
  76. Johns M., Harrington L., Titball R.W., Leslie D.L. Improved methods for the detection of Bacillus anthracis spores by the polymerase chain reaction. Letters in Applied Microbiology. 1994;18:236–238. [Google Scholar]
  77. Jones D.D., Bej A.K. Detection of foodborne microbial pathogens using polymerase chain reaction methods. In: Griffin H.G., Griffin A.M., editors. PCR Technology. Current Innovations. CRC Press; San Diego: 1994. [Google Scholar]
  78. Kaltenboeck B., Kousoulas K.G., Storz J. Two-step polymerase chain reaction and restriction endonuclease analyses detection and differentiation of ompA DNA of Chlamydia spp. Journal of Clinical Microbiology. 1992;30:1098–1104. doi: 10.1128/jcm.30.5.1098-1104.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  79. Kamolvarin N., Tirawatnpong T., Rattanasiwamore R., Tirawatnpong S., Papanich T., Hemachudha T. Diagnosis of rabies by polymerase chain reaction with nested primers. Journal of Infectious Diseases. 1993;167:207–210. doi: 10.1093/infdis/167.1.207. [DOI] [PubMed] [Google Scholar]
  80. Kapperud G., Vardund T., Skjerve E., Hornes E., Michaelsen T.E. Detection of pathogenic Yersinia entercolitica in foods and water by immunomagnetic separation, nested polymerase chain reactions, and colorimetric detection of amplified DNA. Applied and Environmental Microbiology. 1993;59:2938–2944. doi: 10.1128/aem.59.9.2938-2944.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  81. Katz J.B., Shafer A.L., Eernisse K.A., Landgraf J.G., Nelson E.A. Antigenic differences between European and American isolates of porcine reproductive and respiratory syndrome virus (PRRSV) are encoded by the carboxyterminal portion of viral open reading frame 3. Veterinary Microbiology. 1995;44:65–76. doi: 10.1016/0378-1135(94)00113-B. [DOI] [PMC free article] [PubMed] [Google Scholar]
  82. Kawabata H.H., Tashibo H., Yamada K., Masuzawa T., Yanagihara Y. Polymerase chain reaction analysis of Borrelia species isolated in Japan. Microbiology and Immunology. 1993;38:591–598. doi: 10.1111/j.1348-0421.1994.tb01828.x. [DOI] [PubMed] [Google Scholar]
  83. Kelly E.J., Kackson M.J., Marsolais G., Morrey J.D., Callan R.J. Early detection of bovine leukemia virus in cattle by use of the polymerase chain reaction. American Journal of Veterinary Research. 1993;54:205–209. [PubMed] [Google Scholar]
  84. Kempf I., Blanchard A., Gesbert F., Guittet M., Bennejean G. The polymerase chain reaction for the detection of Mycoplasma gallisepticum. Avian Pathology. 1993;22:739–750. doi: 10.1080/03079459308418961. [DOI] [PubMed] [Google Scholar]
  85. Kempf I., Gesbert F., Guittet M., Bennejean G. Mycoplasma gallisepticum infection in drug-treated chickens: comparison of diagnosis methods including polymerase chain reaction. Journal of Veterinary Medicine B. 1994;41:597–602. doi: 10.1111/j.1439-0450.1994.tb00269.x. [DOI] [PubMed] [Google Scholar]
  86. Kennan R., Soderlind O., Conway P. Presence of F107, 2134P and Av24 fimbriae on strains of Escherichia coli isolated from Swedish piglets with diarrhoea. Veterinary Microbiology. 1995;43:123–129. doi: 10.1016/0378-1135(94)00093-c. [DOI] [PubMed] [Google Scholar]
  87. Kimura K., Isogai E., Isogai H., Kamewaka Y., Nishikawa T., Ishii N., Fujii N. Detection of Lyme disease spirochetes in the skin of naturally infected wild sika deer (Cervus nippon yesoensis) by PCR. Applied and Environmental Microbiology. 1995;61:1641–1642. doi: 10.1128/aem.61.4.1641-1642.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  88. Kinenge F.S.B., Harris L.M., McKenna P.K., Wadowska D., Yason C.V. Amplication of strains of bovine herpesvirus 1 by use of polymerase chain reaction with primers in the thymidine kinase region. American Journal of Veterinary Research. 1994;55:1206–1212. [PubMed] [Google Scholar]
  89. Koepel E., Meyer R., Luethy J., Candrian U. Recognition of pathogenic Yersinia enterocolitica by crystal violet binding and polymerase chain reaction. Letters in Applied Microbiology. 1993;17:231–234. doi: 10.1111/j.1472-765x.1993.tb01454.x. [DOI] [PubMed] [Google Scholar]
  90. Kwon H.M., Jackwood M.W., Brown T.P., Hilt D.A. Polymerase chain reaction and a biotin-labeled DNA probe for detection of infectious bronchitis virus in chickens. Avian Diseases. 1993;37:149–156. [PubMed] [Google Scholar]
  91. Kwon H.M., Jackwood M.W., Gelb J. Differentiation of infectious bronchitis virus serotyping using polymerase chain reaction and restriction fragment length polymorphism analysis. Avian Diseases. 1993;37:194–207. [PubMed] [Google Scholar]
  92. La Fontaine S., Egerton J.R., Rood J.I. Detection of Dichelobacter nodosus using species-specific oligonucleotides as PCR primers. Veterinary Microbiology. 1993;35:101–117. doi: 10.1016/0378-1135(93)90119-r. [DOI] [PubMed] [Google Scholar]
  93. Laor O., Torgersen H., Yadin H., Becker Y. Detection of FMDV RNA amplified by the polymerase chain reaction (PCR) Journal of Virological Methods. 1991;36:197–208. doi: 10.1016/0166-0934(92)90051-e. [DOI] [PubMed] [Google Scholar]
  94. Laor O., Yadin H., Chai D., Becker Y. Detection of foot and mouth disease virus in diagnostic material using the PCR method on viral genomic poly-A isolated with oligo dT on magnetic beads. Israel Journal of Veterinary Medicine. 1991;46:127–133. [Google Scholar]
  95. Laxer M.A., Timblin B.K., Patel R.J. DNA sequences for the specific detection of Cryptosporidium parvum by the polymerase chain reaction. American Journal of Tropical Medicine and Hygiene. 1991;45:688–694. doi: 10.4269/ajtmh.1991.45.688. [DOI] [PubMed] [Google Scholar]
  96. Laxer M.A., D'Nicuola M.E., Patel R.J. Detection of Cryptosporidium parvum DNA in fixed paraffin-embedded tissue by polymerase chain reaction. American Journal of Tropical Medicine and Hygiene. 1992;47:450–455. doi: 10.4269/ajtmh.1992.47.450. [DOI] [PubMed] [Google Scholar]
  97. Lee L.H., Yu S.L., Shieh H.K. Detection of infectious bursal disease virus infection using the polymerase chain reaction. Journal of Virological Methods. 1992;40:243–254. doi: 10.1016/0166-0934(92)90083-p. [DOI] [PubMed] [Google Scholar]
  98. Lin Z., Kato A., Kudou Y., Ueda S. A new typing method for the avian infectious bronchitis virus using polymerase chain reaction and restriction fragment length polymorphism. Archives of Virology. 1991;116:19–31. doi: 10.1007/BF01319228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  99. Lipman L.J.A., De Nijs A., Lam T.J.G.M., Gaastra W. Identification of Escherichia coli strains from cows with clinical mastitis by serotyping and DNA polymorphism patterns with REP and ERIC primers. Veterinary Microbiology. 1995;43:13–19. doi: 10.1016/0378-1135(94)00070-d. [DOI] [PubMed] [Google Scholar]
  100. Liu D., Webber J. A polymerase chain reaction assay for improved detection of virulence of Dichelobacter nodosus, the specific causative pathogen of ovine footrot. Veterinary Microbiology. 1995;43:197–207. doi: 10.1016/0378-1135(94)00091-a. [DOI] [PubMed] [Google Scholar]
  101. Liu S.T., Li S.N., Wang D.C., Chang S.F., Chiang S.C., Ho W.C., Chang Y.S., Lai S.S. Rapid detection of hog cholera virus in tissues by polymerase chain reaction. Journal of Virological Methods. 1991;35:227–236. doi: 10.1016/0166-0934(91)90138-p. [DOI] [PubMed] [Google Scholar]
  102. Lopez M., Inga R., Cangalaya M., Echevarria J., Llanos-Cuentas A., Orrego C., Arevalo J. Diagnosis of Leishmania using the polymerase chain reaction: a simplified procedure for field work. American Journal of Tropical Medical and Hygiene. 1993;49:348–356. doi: 10.4269/ajtmh.1993.49.348. [DOI] [PubMed] [Google Scholar]
  103. MacLachlan N.J., Nunamaker R.A., Katz J.B. Detection of bluetongue virus in the blood of inoculated calves: comparison of virus isolation, PCR assay, and in vitro feeding of Cullicoidesvariipennis. Archives of Virology. 1994;136:1–8. doi: 10.1007/BF01538812. [DOI] [PubMed] [Google Scholar]
  104. MacPherson J.M., Gajadhar A.A. Sensitive and specific polymerase chain reaction detection of Toxoplasma gondii for veterinary and medical diagnosis. Canadian Journal of Veterinary Research. 1993;57:45–48. [PMC free article] [PubMed] [Google Scholar]
  105. Mahbubani M.H., Bej A.K. Application of polymerase chain reaction methodology in clinical diagnosis. In: Griffin H.G., Griffin A.M., editors. PCR Technology. Current Innovations. CRC Press; Boca Raton: 1994. [Google Scholar]
  106. Mahon J., Lax A.J. A quantitative polymerase chain reaction method for the detection in avian feces of salmonellas carrying the spvR gene. Epidemiology and Infection. 1993;111:455–464. doi: 10.1017/s0950268800057186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  107. Mamaev L.V., Denikina N.N., Belikov S.I., Volchkov V.E., Visser I.K.G., Fleming M., Kai C., Harder T.C., Liess B., Osterhaus A.D.M.E., Barrett T. Characterisation of morbilliviruses isolated from Lake Baikal seals (Phoca sibirica) Veterinary Microbiology. 1995;44:251–259. doi: 10.1016/0378-1135(95)00018-6. [DOI] [PubMed] [Google Scholar]
  108. Marconi R.T., Garon C.F. Development of polymerase chain reaction primers sets for diagnosis of Lyme disease and for species-specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis. Journal of Clinical Microbiology. 1992;30:2830–2834. doi: 10.1128/jcm.30.11.2830-2834.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  109. Marin M.S., McKenzie J., Gao G.F., Reid H.W., Antoniadis A., Gould E.A. The virus causing encephalomyelitis in sheep in Spain: a new member of the tick-borne encephalitis group. Research in Veterinary Science. 1995;58:11–13. doi: 10.1016/0034-5288(95)90081-0. [DOI] [PubMed] [Google Scholar]
  110. McColl K.A., Gould A.R. Detection and characterization of bluetongue virus using the polymerase chain reaction. Virus Research. 1991;21:19–34. doi: 10.1016/0168-1702(91)90069-8. [DOI] [PubMed] [Google Scholar]
  111. McColl K.A., Gould A.R. Bluetongue virus infection in sheep: haemotological changes and detection by polymerase chain reaction. Australian Veterinary Journal. 1994;71:97–101. doi: 10.1111/j.1751-0813.1994.tb03346.x. [DOI] [PubMed] [Google Scholar]
  112. McColl K.A., Gould A.R., Selleck P.W., Hooper P.T., Westbury H.A., Smith J.S. Polymerase chain reaction and other laboratory techniques in the diagnosis of long incubation rabies in Australia. Australian Veterinary Journal. 1993;70:84–89. doi: 10.1111/j.1751-0813.1993.tb03282.x. [DOI] [PubMed] [Google Scholar]
  113. Merien F., Amourioux P., Perolat P., Baranton G., Saint Girons I. Polymerase chain reaction for the detection of Leptospira spp. in clinical samples. Journal of Clinical Microbiology. 1992;30:2219–2224. doi: 10.1128/jcm.30.9.2219-2224.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  114. Meyer R.F., Brown C., Jouse J.A., Miltor T.W. Rapid and sensitive detection of foot and mouth disease virus in tissues by enzymatic RNA amplification of the polymerase gene. Journal of Virological Methods. 1991;34:161–172. doi: 10.1016/0166-0934(91)90096-i. [DOI] [PubMed] [Google Scholar]
  115. Molitor T.W., Oraveerakul K., Zhang Q.Q., Choi C.S., Ludemann L.R. Polymerase chain reaction (PCR) amplification for the detection of porcine parvovirus. Journal of Virological Methods. 1991;32:201–211. doi: 10.1016/0166-0934(91)90051-z. [DOI] [PubMed] [Google Scholar]
  116. Murtaugh M.P., Lin G.F., Haggard D.L., Webber A.F., Meiske J.C. Detection of bovine leukemia virus in cattle by the polymerase chain reaction. Journal of Virological Methods. 1991;33:73–85. doi: 10.1016/0166-0934(91)90009-o. [DOI] [PubMed] [Google Scholar]
  117. Naif H.M., Brandon R.B., Daniel R.C.W., Lavin M.F. Bovine leukaemia provirae DNA detection in cattle by the polymerase chain reaction. Veterinary Microbiology. 1990;25:117–129. doi: 10.1016/0378-1135(90)90071-3. [DOI] [PubMed] [Google Scholar]
  118. Naif H.M., Daniel R.C.W., Cougle W.G., Lavin M.F. Early detection of bovine leukaemia virus by using an enzyme-linked assay for polymerase chain reaction-amplified proviral DNA in experimentally infected cattle. Journal of Clinical Microbiology. 1992;30:675–679. doi: 10.1128/jcm.30.3.675-679.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  119. Nascimento E.R., Yamamoto R., Herrick K.R., Tait R.C. Polymerase chain reaction for the detection of Mycoplasma gallisepticum. Avian Diseases. 1991;35:62–69. [PubMed] [Google Scholar]
  120. Nash J.W., Hanson L.A., Coats K.S.C. Detection of bovine immunodeficiency virus in blood and milk-derived leukocytes by use of polymerase chain reaction. American Journal of Veterinary Research. 1995;56:445–449. [PubMed] [Google Scholar]
  121. Nguyen A.V., Khan M.I., Lu Z. Amplification of salmonella chromosomal DNA using the polymerase chain reaction. Avian Diseases. 1994;38:119–126. [PubMed] [Google Scholar]
  122. Nunberg J.H., Wright D.K., Cole G.E., Petrovski E.A., Post L.E., Compton T., Gilbert J.H. Identification of the thymidine kinase gene of feline herpesvirus: use of degenerate oligonucleotides in the polymerase chain reaction to isolateherpesvirus gene homologies. Journal of Virology. 1989;63:3240–3249. doi: 10.1128/jvi.63.8.3240-3249.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  123. Ojeniyl B., Ahrens P., Meyling A. Detection of fimbrial and toxin genes in Escherichia coli and their prevalence in piglets with diarrhea. The application of colony hybridization assay, polymerase chain reaction and phenotypic assays. Journal of Veterinary Medicine B. 1994;41:49–59. doi: 10.1111/j.1439-0450.1994.tb00205.x. [DOI] [PubMed] [Google Scholar]
  124. Olsen B., Jaenson T.G.T., Bergstrom S. Prevalence of Borrelia burgdorferi sensu lato-infected ticks on migrating birds. Applied and Environmental Microbiology. 1995;61:3082–3087. doi: 10.1128/aem.61.8.3082-3087.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  125. O'Rourke K.I., Besola M.L., McGuire T.C. Proviral sequences detected by the polymerase chain reaction in peripheral blood cells of horses with equine infectious anemia lentivirus. Archives of Virology. 1991;117:109–119. doi: 10.1007/BF01310496. [DOI] [PubMed] [Google Scholar]
  126. Ouahrani S., Michaux S., Widada J.S., Bourg G., Tournebize R., Ramuz M., Liautard J.P. Identification and sequence analysis of IS6501, an insertion sequence in Brucella spp.: relationship between genomic structure and the numberof IS6501 copies. Journal of General Microbiology. 1993;139:3265–3273. doi: 10.1099/00221287-139-12-3265. [DOI] [PubMed] [Google Scholar]
  127. Parsonson I.M., Thompson L.H., Walton T.E. Experimentally induced infection with bluetongue virus serotype II in cows. American Journal of Veterinary Research. 1994;55:1529–1534. [PubMed] [Google Scholar]
  128. Paton D.J., Carisson U., Lowings J.P., Sands J.J., Vilcek S., Alenius S. Identification of herd-specific bovine viral diarrhea virus isolates from infected cattle and sheep. Veterinary Microbiology. 1995;43:283–294. doi: 10.1016/0378-1135(94)00107-8. [DOI] [PubMed] [Google Scholar]
  129. Peters M., Pohlenz J., Jaton K., Ninet B., Bille J. Studies of the detection of Listeria monocytogenes by culture and PCR in cerebrospinal fluid samples from ruminants with listeric encephalitis. Journal of Veterinary Medicine B. 1995;42:84–88. doi: 10.1111/j.1439-0450.1995.tb00686.x. [DOI] [PubMed] [Google Scholar]
  130. Procunier J.D., Fernando M.A., Barta J.R. Species and strain differentiation of Eimeria spp. of the domestic fowl using DNA polymorphisms amplified by arbitrary primers. Parasitology Research. 1993;79:98–102. doi: 10.1007/BF00932253. [DOI] [PubMed] [Google Scholar]
  131. Radwan G.S., Brock K.V., Hogan J.S., Smith K.L. Development of a PCR amplification assay as a screening test using bulk milk samples for identifying dairy herds infected with bovine viral diarrhea virus. Veterinary Microbiology. 1995;44:77–92. doi: 10.1016/0378-1135(94)00121-c. [DOI] [PubMed] [Google Scholar]
  132. Rahn K.A., De Granchis A., Clarke R.C., McEwen S.A., Galan J.E., Ginocchio C., Curtiss R., Gyles C.L. Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Molecular and Cellular Probes. 1992;6:271–279. doi: 10.1016/0890-8508(92)90002-f. [DOI] [PubMed] [Google Scholar]
  133. Rasmussen H.N., Rasmussen O.F., Andersen J.K., Olsen J.E. Specific detection of pathogenic Yersinia enterocolitica by two-step PCR using hot-start and DMSO. Molecular and Cellular Probes. 1994;8:99–108. doi: 10.1006/mcpr.1994.1014. [DOI] [PubMed] [Google Scholar]
  134. Rasmussen H.N., Rasmussen O.F., Christensen H., Olsen J.E. Detection of Yersinia enterocolitica O:3 in faecal samples and tonsil swabs from pigs using IMS and PCR. Journal of Applied Bacteriology. 1995;78:563–568. doi: 10.1111/j.1365-2672.1995.tb03100.x. [DOI] [PubMed] [Google Scholar]
  135. Reif T.C., Johns M., Pillai S., Carl M. Identification of capsule-forming Bacillus anthracis spores with the PCR and a novel dual probe hybridization format. Applied and Environmental Microbiology. 1994;60:1622–1625. doi: 10.1128/aem.60.5.1622-1625.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  136. Reubel G.H., Ramos R.A., Hickman M.A., Rimsead E., Hoffmann D.E., Pedersen N.C. Detection of active and latent feline herpesvirus 1 infections using the polymerase chain reaction. Archives of Virology. 1993;132:409–420. doi: 10.1007/BF01309549. [DOI] [PubMed] [Google Scholar]
  137. Ridge S.E., Harkin J.T., Badman R.T., Mellor A.M., Larsen J.W.A. Johne's disease in alpacas (Lama pacos) in Australia. Australian Veterinary Journal. 1995;72:150–153. doi: 10.1111/j.1751-0813.1995.tb15040.x. [DOI] [PubMed] [Google Scholar]
  138. Rimslad G.H., Ueland K. Detection of feline immunodeficiency virus by a nested polymerase chain reaction. Journal of Virological Methods. 1992;36:239–248. doi: 10.1016/0166-0934(92)90055-i. [DOI] [PubMed] [Google Scholar]
  139. Rishi A.K., McManus D.P. Genomic cloning of human Echinococcus granulosus DNA isolation of recombinant plasmids and their use as genetic markers in strain characterization. Parasitology. 1987;94:369–383. doi: 10.1017/s0031182000054020. [DOI] [PubMed] [Google Scholar]
  140. Rishi A.K., McManus D.P. Molecular cloning of Taenia solium genomic DNA and characterization of taeniid cestodes by DNA analysis. Parasitology. 1988;97:161–176. doi: 10.1017/s003118200006683x. [DOI] [PubMed] [Google Scholar]
  141. Rodriguez A., Martinez-Salas E., Dopazo J., Davila M., Saiz J.C., Sobrino F. Primer design for specific diagnosis by PCR of highly variable RNA viruses: typing of foot-and-mouth disease virus. Virology. 1992;189:363–367. doi: 10.1016/0042-6822(92)90717-4. [DOI] [PubMed] [Google Scholar]
  142. Rosa P.A., Schwan T.G. A specific and sensitive assay for the Lyme disease spirochete Borrelia burgdorferi using the polymerase chain reaction. Journal of Infectious Diseases. 1989;160:1018–1029. doi: 10.1093/infdis/160.6.1018. [DOI] [PubMed] [Google Scholar]
  143. Roze M. Polymerase chain reaction: a revolution in diagnosis of ocular leishmaniosis? Veterinary Quarterly. 1995;17:547–548. [PubMed] [Google Scholar]
  144. Sacramento D., Bourhy H., Tordo N. PCR technique as an alternative method for diagnosis and molecular epidemiology of rabies virus. Molecular and Cellular Probes. 1991;5:299–340. doi: 10.1016/0890-8508(91)90045-l. [DOI] [PubMed] [Google Scholar]
  145. Saiki R.K., Scharf S., Faloona F., Mullin K.B., Horn G.T., Erlich H.A., Arnheim N. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985;230:1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]
  146. Saiki R.K., Gelfand D.H., Stoffel S., Scharf S., Higucchi R., Horn G.T., Mullis K.B., Erlich H.A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988;239:487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  147. Saito M., Matsumoto M., Funabashi M. Detection of Clostridium perfringens enterotoxin gene by the polymerase chain reaction amplification procedure. International Journal of Food Microbiology. 1992;17:47–55. doi: 10.1016/0168-1605(92)90018-x. [DOI] [PubMed] [Google Scholar]
  148. Sarid R., Chajut A., Malkinson M., Tronick S.R., Gazit A., Yaniv A. Diagnostic test for lymphoproliferative disease virus infection of turkeys, using the polymerase chain reaction. American Journal of Veterinary Research. 1994;55:769–772. [PubMed] [Google Scholar]
  149. Saurnier P., Bourneux C., Prevost G., Andremont A. RAPD assay is less discriminant than pulse-field gel electrophoresis for typing strains of methicillin-resistant Staphylococcus aureus. Journal of Clinical Microbiology. 1993;31:982–985. doi: 10.1128/jcm.31.4.982-985.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  150. Sayada C., Andersen A., Rodriguez P., Eb F., Milon A., Elion J., Denamur E. Homogeneity of the major outer membrane protein gene of feline Chlamydia psittaci. Research in Veterinary Science. 1994;56:116–118. doi: 10.1016/0034-5288(94)90207-0. [DOI] [PubMed] [Google Scholar]
  151. Schorr E., Wentworth D., Hinshaw V.S. Use of polymerase chain reaction to detect swine influenza virus in nasal swab specimens. American Journal of Veterinary Research. 1994;55:952–956. [PubMed] [Google Scholar]
  152. Schunck B., Rziha H.J. Detection of food-borne viruses by the polymerase chain reaction. Revue du Médicine Veterinaire. 1994;145:215–216. [Google Scholar]
  153. Senda M., Parrish C.R., Harasawa R., Gamoh K., Muramatsu M., Hirayama N., Itho O. Detection by PCR of wild-type canine parvovirus which contaminates dog vaccines. Journal of Clinical Microbiology. 1995;33:110–113. doi: 10.1128/jcm.33.1.110-113.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  154. Shankar V., Dietzschold B., Koprowski H. Direct entry of rabies virus into the central nervous system without prior local replication. Journal of Virology. 1991;65:2736–2738. doi: 10.1128/jvi.65.5.2736-2738.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  155. Sharma P.C., Cullinane A.A., Onions D.E., Nicolson L. Diagnosis of equid herpesvirus 1 and 4 by polymerase chain reaction. Equine Veterinary Journal. 1992;24:20–25. doi: 10.1111/j.2042-3306.1992.tb02772.x. [DOI] [PubMed] [Google Scholar]
  156. Sherman M., Ehrlich G.D., Ferrer J.F., Sninsky J.J., Zandomeni R., Dock N.I., Poiesz B. Amplification and analysis of specific DNA and RNA sequences of bovine leukemia virus from infected cows by polymerase chain reaction. Journal of Clinical Microbiology. 1992;30:185–191. doi: 10.1128/jcm.30.1.185-191.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  157. Siles-Lucas M., Felleisen L., Cuesta-Bandera C., Gottstein B., Eckert J. Comparative genetic analysis of Swiss and Spanish isolates of Echinococcus granulosus by Southern hybridization and randomly amplified polymorphic DNA technique. Applied Parasitology. 1994;35:107–117. [PubMed] [Google Scholar]
  158. Silva R.F. Differentiation of pathogenic and non-pathogenic serotype 1 Marek's disease viruses (MDVs) by the polymerase chain reaction amplification of the tandem direct repeats within the MDV genome. Avian Diseases. 1992;36:521–528. [PubMed] [Google Scholar]
  159. Smith H.R., Scotland S.M., Willshaw G.A., Wray C., McLaren I.M., Cheasty T., Rowe B. Verocytotoxin production and presence of VT genes in Escherichia coli strains of animal origin. Journal of General Microbiology. 1988;134:829–834. doi: 10.1099/00221287-134-3-829. [DOI] [PubMed] [Google Scholar]
  160. Smyth A.J., Ghosh A., Hassan M.Q., Bassu D., De Bruijn S., Adhya S., Mallick K.K., Barker D.C. Rapid and sensitive detection of Leishmania kenetoplast DNA from spleen and blood samples of kala-azar patients. Parasitology. 1992;105:183–192. doi: 10.1017/s0031182000074096. [DOI] [PubMed] [Google Scholar]
  161. Solano P., Argiro L., Reifenberg J.M., Duvallet G. Utilisation de l'hybridisation moléculaire et de l'amplification en chaïne par polymérase pour la charactérisation des trypanosomes et l'epidemiologie des trypanosomes en Afrique de l'Quest. Veterinary Research. 1994;25:588–589. [Google Scholar]
  162. Stanley J., Linton D., Burnens A.P., Dewhirst F.E., Owen R.J., Porter A., On S.L.W., Costas M. Helicobacter canis, sp. nov, a new species from dogs: an integrated study of phenotype and genotype. Journal of General Microbiology. 1993;139:2495–2504. doi: 10.1099/00221287-139-10-2495. [DOI] [PubMed] [Google Scholar]
  163. Staskus K.A., Couch L., Bitterman P., Retzel E.F., Zupangic M., List J., Haase A.T. In situ amplification of visna virus DNA in tissue sections reveals a reservoir of latently infected cells. Microbial Pathogenesis. 1991;11:67–76. doi: 10.1016/0882-4010(91)90095-r. [DOI] [PubMed] [Google Scholar]
  164. Steiger Y., Ackermann M., Mettraux C., Kihm U. Rapid and biologically safe diagnosis of african swine fever by using polymerase chain reaction. Journal of Clinical Microbiology. 1992;30:1–8. doi: 10.1128/jcm.30.1.1-8.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  165. Stemke G.W., Phan R., Young T.F., Ross R.F. Differentiation of Mycoplasma hyopneumoniae, Mycoplasma flocculare, and Mycoplasma hyorhinis on the basis of amplification of a 16S rRNA genesequence. American Journal of Veterinary Research. 1994;55:81–84. [PubMed] [Google Scholar]
  166. Stettler M., Zurbriggen A. Nucleotide and deduced amino acid sequence of the nucleocapsid protein of the virulent A75/17-CDV strain of canine distemper virus. Veterinary Microbiology. 1995;44:211–217. doi: 10.1016/0378-1135(95)00014-2. [DOI] [PubMed] [Google Scholar]
  167. Stone-Marschal M., Carville A., Skowronek A., Lafgreid W.W. Detection of African horse sickness virus by reverse transcription-PCR. Journal of Clinical Microbiology. 1994;32:697–700. doi: 10.1128/jcm.32.3.697-700.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  168. Stram Y., Yadin H., Chai D., Molad T., Gelman B., Laor O., Becker Y. Identification of foot and mouth disease virus (FMDV) serotypes using the polymerase chain reaction. Israel Journal of Veterinary Medicine. 1993;48:57–60. [Google Scholar]
  169. Stram Y., Molad T., Chai D., Gelman B., Yadin H. Detection and subtyping of foot-and-mouth disease virus in infected cattle by polymerase chain reaction and amplified VP1 sequencing. Journal of Veterinary Diagnostic Investigation. 1995;7:52–55. doi: 10.1177/104063879500700107. [DOI] [PubMed] [Google Scholar]
  170. Suarez D.L., Van Der Maaten M.J., Whetstone C.A. Improved early and long-term detection of bovine lentivirus by a nested polymerase chain reaction test in experimentally infected calves. American Journal of Veterinary Research. 1995;56:579–586. [PubMed] [Google Scholar]
  171. Szabo E.A., Pemberton J.M., Desmarchelier P.M. Specific detection of Clostridium botulinum type B by using the polymerase chain reaction. Applied and Environmental Microbiology. 1992;58:418–420. doi: 10.1128/aem.58.1.418-420.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  172. Szabo E.A., Pemberton J.M., Desmarchelier P.M. Detection of the genes encoding botulinum neurotoxin types A to E by the polymerase chain reaction. Applied and Environmental Microbiology. 1993;59:3011–3020. doi: 10.1128/aem.59.9.3011-3020.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  173. Tajima M., Kirisawa R., Taguchi M., Iwai H., Kawakami Y., Hagiwara K., Ohtsuka H., Sentsui H., Takahashi K. Attempt to discriminate between bovine viral diarrhea virus strains using polymerase chain reaction. Journal of Veterinary Medicine B. 1995;42:257–265. doi: 10.1111/j.1439-0450.1995.tb00709.x. [DOI] [PubMed] [Google Scholar]
  174. Truyen U., Platzer G., Parrish C.R., Hanichen T., Hermanns W., Kaaden O.R. Detection of canine parvovirus DNA in paraffin-embedded tissues by polymerase chain reaction. Journal of Veterinary Medicine. 1994;41:148–152. doi: 10.1111/j.1439-0450.1994.tb00218.x. [DOI] [PubMed] [Google Scholar]
  175. Turnbull P.C.B., Huston R.A., Ward M.J., Jones M.N., Quinn C.P., Finnie N.J., Duggleby C.J., Kramer J.M., Melling J. Bacillus anthracis but not always anthrax. Journal of Applied Bacteriology. 1992;72:21–28. doi: 10.1111/j.1365-2672.1992.tb04876.x. [DOI] [PubMed] [Google Scholar]
  176. Tyler S.D., Johnson W.M., Lior H., Wang G., Rozee K.R. Identification of verotoxin type 2 variant B subunit genes in Escherichia coli by the polymerase chain reaction and restriction fragment lengthpolymorphism analysis. Journal of Clinical Microbiology. 1991;29:1339–1343. doi: 10.1128/jcm.29.7.1339-1343.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  177. Uwatoko K., Sunairi M., Nakajima M., Yamaura K. Rapid method utilizing polymerase chain reaction for detection of canine parvovirus in feces of diarrheic dogs. Veterinary Microbiology. 1995;43:315–323. doi: 10.1016/0378-1135(94)00102-3. [DOI] [PubMed] [Google Scholar]
  178. Van Belkum A., Bax R., Peerbooms P., Goessens W., Van Leeuwen N., Quint W.G.V. Comparison of phage typing and DNA fingerprinting by PCR for discrimination of methicillin-resistant Staphylococcus aureus strains. Journal of Clinical Microbiology. 1993;31:798–803. doi: 10.1128/jcm.31.4.798-803.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  179. Van Eys G.J.J.M., Gravekamp C., Gerritsen M.J., Quint W., Cornelissen M.T.E., Ter Schegget J., Terpstra W.J. Detection of leptospires in urine by polymerase chain reaction. Journal of Clinical Microbiology. 1989;27:2258–2262. doi: 10.1128/jcm.27.10.2258-2262.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  180. Van Eys G.J.J.M., Schoone G.J., Kroon N.C.M., Ebelling S.B. Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites. Molecular and Biochemical Parasitology. 1992;51:133–142. doi: 10.1016/0166-6851(92)90208-2. [DOI] [PubMed] [Google Scholar]
  181. Van Woensel P.A.M., Van Blaaderen A., Moormann R.J.M., De Boer G.F. Detection of proviral DNA and viral RNA in various tissues early after avian leukosis infection. Leukemia. 1992;6:135S–137S. [PubMed] [Google Scholar]
  182. Venugopal K., Shui S.Y.W., Gould E.A. Recombinant vaccinia virus expressing PrM and E glycoproteins of louping-ill virus: induction of partial homologous and heterologous protection in mice. Research in Veterinary Science. 1994;57:188–193. doi: 10.1016/0034-5288(94)90056-6. [DOI] [PubMed] [Google Scholar]
  183. Verbeek A., Tijssen P. Polymerase chain reaction for probe synthesis and for direct amplification in detection of bovine coronavirus. Journal of Virological Methods. 1990;29:243–256. doi: 10.1016/0166-0934(90)90052-H. [DOI] [PMC free article] [PubMed] [Google Scholar]
  184. Vilcek S. Detection of bovine herpesvirus-1 (BHV-1) genome by PCR. Journal of Virological Methods. 1993;41:245–248. doi: 10.1016/0166-0934(93)90132-b. [DOI] [PubMed] [Google Scholar]
  185. Visser I.K.G., Kumarev V.P., Orvell C., Vries P.D., Broeders H.W.J., Bildt M.W.G.V., Groen J., Teppema J.S. Comparison of two morbilli-viruses isolated from seals during outbreaks of distemper in North West Europe and Siberia. Archives of Virology. 1990;111:149–164. doi: 10.1007/BF01311050. [DOI] [PubMed] [Google Scholar]
  186. Volz D.M., Lager K.M., Mengeling W.L. Latency of a thymidine kinase-negative pseudorabies vaccine virus detected by the polymerase chain reaction. Archives of Virology. 1992;122:341–348. doi: 10.1007/BF01317195. [DOI] [PubMed] [Google Scholar]
  187. Wade-Evans A.M., Mertens P.P., Bostock C.J. Development of the polymerase chain reaction for the detection of bluetongue virus in tissue samples. Journal of Virological Methods. 1991;30:15–24. doi: 10.1016/0166-0934(90)90040-m. [DOI] [PubMed] [Google Scholar]
  188. Wagner W.N., Bogdan J., Haines D., Townsend H.G.G., Misra V. Detection of equine herpesvirus and differentiation of equine herpesvirus type 1 and type 4 by the polymerase chain reaction. Canadian Journal of Microbiology. 1992;38:1193–1196. doi: 10.1139/m92-196. [DOI] [PubMed] [Google Scholar]
  189. Wamwayi H.M., Fleming M., Barrett T. Characterisation of African isolates of rindepest virus. Veterinary Microbiology. 1995;44:151–163. doi: 10.1016/0378-1135(95)00008-x. [DOI] [PubMed] [Google Scholar]
  190. Ward P., Misra V. Detection of bovine viral diarrhea virus using degenerate oligonucleotides primers and the polymerase chain reaction. American Journal of Veterinary Research. 1991;52:1231–1236. [PubMed] [Google Scholar]
  191. Wards B.J., Collins D.M., De Lisle G.W. Detection of Mycobacterium bovis in tissues by polymerase chain reaction. Veterinary Microbiology. 1995;43:227–240. doi: 10.1016/0378-1135(94)00096-f. [DOI] [PubMed] [Google Scholar]
  192. Wastling J.M., Nicolls S., Buxton D. Comparation of two gene amplification methods for the detection of Toxoplasma gondii in experimentally infected sheep. Journal of Medical Microbiology. 1993;38:360–365. doi: 10.1099/00222615-38-5-360. [DOI] [PubMed] [Google Scholar]
  193. Way J.J., Josephson K.L., Pillai S.D., Abbaszadegan M., Gerba C.P., Pepper I.L. Specific detection of Salmonella spp. by multiplex polymerase chain reaction. Applied and Environmental Microbiology. 1993;59:1473–1479. doi: 10.1128/aem.59.5.1473-1479.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  194. Weiss J.B. DNA probes and PCR for diagnosis of parasitic infections. Clinical Microbiology Reviews. 1995;8:113–130. doi: 10.1128/cmr.8.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  195. Wheeler J.G., Osorio F.A. Investigation of sites of pseudorabies virus latency, using polymerase chain reaction. American Journal of Veterinary Research. 1991;52:1799–1803. [PubMed] [Google Scholar]
  196. Widjojoatmodjo M.N., Flutt A.C., Torensma R., Verdonk G.P.H.T., Verhoef J. The magnetic immuno-polymerase chain reaction assay for direct detection of salmonellae in fecal samples. Journal of Clinical Microbiology. 1992;30:3195–3199. doi: 10.1128/jcm.30.12.3195-3199.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  197. Wiedman M., Brandon R., Wagner P., Dubovi E., Batt C.A. Detection of bovine herpesvirus-1 in bovine semen by a nested PCR. Journal of Virological Methods. 1993;44:129–140. doi: 10.1016/0166-0934(93)90015-j. [DOI] [PubMed] [Google Scholar]
  198. Willems H., Thiele D., Frolich-Ritter R., Krauss H. Detection of Coxiella burnetti in cow's milk using the polymerase chain reaction. Journal of Veterinary Medicine B. 1994;41:580–587. doi: 10.1111/j.1439-0450.1994.tb00267.x. [DOI] [PubMed] [Google Scholar]
  199. Wilson W.C., Chase C.C. Nested multiplex polymerase chain reactions for the identification of bluetongue virus infection in the biting midge Cullicoides variipennis. Journal of Virological Methods. 1993;45:39–47. doi: 10.1016/0166-0934(93)90138-h. [DOI] [PubMed] [Google Scholar]
  200. Wirz B., Tratschin J., Muller H.K., Mitchell D.B. Detection of hog cholera virus and differentiation from other pestiviruses by polymerase chain reaction. Journal of Clinical Microbiology. 1993;31:1148–1154. doi: 10.1128/jcm.31.5.1148-1154.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  201. Wood M.W., Mahon J., Lax A.J. Development of a probe and PCR primers specific to the virulence plasmid of Salmonella enteritidis. Molecular and Cellular Probes. 1994;8:473–479. doi: 10.1006/mcpr.1994.1068. [DOI] [PubMed] [Google Scholar]
  202. Woodward M.J., Carroll P.J., Wray C. Detection of entero- and verocyto-toxin genes in Escherichia coli from diarrhoeal disease in animals using the polymerase chain reaction. Veterinary Microbiology. 1992;31:251–261. doi: 10.1016/0378-1135(92)90083-6. [DOI] [PubMed] [Google Scholar]
  203. Wu C.C., Lin T.L. Detection of infectious bursal disease virus in digested formalin-fixed paraffin embedded tissue sections by polymerase chain reaction. Journal of Veterinary Diagnostic Investigation. 1992;4:452–455. doi: 10.1177/104063879200400416. [DOI] [PubMed] [Google Scholar]
  204. Wu C.C., Lin T.L., Zhang H.G., Davis V.S., Boyle J.A. Molecular detection of infectious bursal disease virus by polymerase chain reaction. Avian Diseases. 1992;36:221–226. [PubMed] [Google Scholar]
  205. Xia J.Q., Yason C.V., Kibenge F.S.B. Comparison of dot-blot hybridization, polymerase chain reaction, and virus isolation for detection of bovine herpesvirus-1 (BHV-1) inartificially-infected bovine semen. Canadian Journal of Veterinary Research. 1995;59:102–109. [PMC free article] [PubMed] [Google Scholar]
  206. Xu L., Harbour D., McCrae M.A. The application of polymerase chain reaction to the detection of rotaviruses in faeces. Journal of Virological Methods. 1990;27:29–38. doi: 10.1016/0166-0934(90)90143-4. [DOI] [PubMed] [Google Scholar]
  207. Yason C.V., Harris L.M., McKenna P.K., Wadowska D., Kibenge F.S.B. Establishment of conditions for the detection of bovine herpesvirus-1 by polymerase chain reaction using primers in the thymidine kinase region. Canadian Journal of Veterinary Research. 1995;59:94–101. [PMC free article] [PubMed] [Google Scholar]
  208. Yule A. Amplification-based diagnostic target TB. Bacteriology. 1994;12:1335–1337. doi: 10.1038/nbt1294-1335. [DOI] [PubMed] [Google Scholar]
  209. Zanoni R.G., Nauta I.M., Kuhnert P., Pauli U., Pohl B., Peterhans E. Genomic heterogeneity of small ruminant lentiviruses detected by PCR. Veterinary Microbiology. 1992;33:241–251. doi: 10.1016/0378-1135(92)90061-w. [DOI] [PubMed] [Google Scholar]
  210. Zerbes M., Tannock G.A., Jenner R.J., Young P.L. Some characteristics of a recent virus isolate of Marek's disease virus. Australian Veterinary Journal. 1994;71:21–22. doi: 10.1111/j.1751-0813.1994.tb00897.x. [DOI] [PubMed] [Google Scholar]
  211. Zhu G.S., Ojima T., Hirowaka T., Ihara T., Mizukoshi N., Kato A., Ueda S., Hirai K. Differentiation of oncogenic and nononcogenic strains of Marek's disease virus type 1 by using polymerase chain reaction DNA amplification. Avian Diseases. 1992;36:637–645. [PubMed] [Google Scholar]
  212. Zientara S., Sailleau C., Moulay S., Cruciere C. Diagnosis of the African horse sickness virus serotype 4 by a one-tube, one manipulation RT-PCR reaction from infected organs. Journal of Virological Methods. 1993;46:133–143. doi: 10.1016/0166-0934(94)90102-3. [DOI] [PubMed] [Google Scholar]
  213. Zingg B.C., Lefebvre R.B. Polymerase chain reaction for detection of Borrelia coriaceae, putative agent of epizootic bovine abortion. American Journal of Veterinary Research. 1994;55:1509–1515. [PubMed] [Google Scholar]
  214. Zwaagstra K.A., Van Der Zeijst B.A.M., Kusters J.G. Rapid detection and identification of avian infectious bronchitis virus. Journal of Clinical Microbiology. 1992;30:79–84. doi: 10.1128/jcm.30.1.79-84.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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