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. 2006 Jun 6;114(3):315–323. doi: 10.1016/S0021-9975(96)80053-3

Detection of porcine reproductive and respiratory syndrome virus and Mycoplasma hyorhinis antigens in pulmonary lesions of pigs suffering from respiratory distress

K Kawashima 1, S Yamada 1, H Kobayashi 1, M Narita 1
PMCID: PMC7130235  PMID: 8762589

Summary

Eleven field cases of a disease characterized by severe dyspnoea or abdominal breathing were examined post mortem. The affected pigs had antibody against porcine reproductive and respiratory syndrome virus (PRRSV). The predominant lung lesions were severe proliferative and interstitial pneumonia, and slight suppurative bronchopneumonia. The lesions were closely associated with the sites at which PRRSV and Mycoplasma hyorhinis antigens were detected. Four of five pigs inoculated with PRRSV developed slight pneumonitis. The fifth animal, which died of severe pneumonitis, yielded a heavy culture of M. hyorhinis. These findings demonstrate that dual infection with M. hyorhinis and PRRSV caused severe pulmonary lesions.

References

  1. Aluotto B.B., Wittler R.G., Williams C.O., Faber J.E. Standardized bacteriologic techniques for the characterization of Mycoplasma species. International Journal of Systematic Bacteriology. 1970;20:35–58. [Google Scholar]
  2. Christensen G., Mousing J. Causal factors in respiratory disease. In: Leman A.D., Straw B.E., Mengeling W.L., D'Allaire S., Taylor D.J., editors. Disease of Swine. 7th Edit. Iowa State University Press; Ames, Iowa: 1992. pp. 142–148. [Google Scholar]
  3. Dea S., Bilodeau R., Athanassious R., Sauvageau R., Martineau G.P. Swine reproductive and respiratory syndrome in Quebec: isolation of an enveloped virus serologically related to Lelystad virus. Canadian Veterinary Journal. 1992;33:801–808. [PMC free article] [PubMed] [Google Scholar]
  4. De Jong M.F., Cromwijk W., Van't Veld P. The “new” pig disease: epidemiology and production losses in the Netherlands. EEC Seminar Report on the New Pig Disease: Porcine Reproductive and Respiratory Syndrome; PRRS, Brussels; 1991. pp. 9–19. [Google Scholar]
  5. Done S.H., Paton D.J. Porcine reproductive and respiratory syndrome: clinical disease, pathology and immunosuppression. Veterinary Record. 1995;136:32–35. doi: 10.1136/vr.136.2.32. [DOI] [PubMed] [Google Scholar]
  6. Friis N.F. Mycoplasma hyorhinis as a causative agent in pneumonia of pigs. Acta Veterinaria Scandinavica. 1971;12:116–119. [PubMed] [Google Scholar]
  7. Galina L., Pijoan C., Sitjar M., Christianson W.T., Rossow K., Collins J.E. Interaction between Streptococcus suis serotype 2 and porcine reproductive and respiratory syndrome virus in specific pathogen-free piglets. Veterinary Record. 1994;134:60–64. doi: 10.1136/vr.134.3.60. [DOI] [PubMed] [Google Scholar]
  8. Gois M., Pospisil Z., Cerny M., Mrva V. Production of pneumonia after intranasal inoculation of gnotobiotic piglets with three strains of Mycoplasma hyorhinis. Journal of Comparative Pathology. 1971;81:401–410. doi: 10.1016/0021-9975(71)90028-4. [DOI] [PubMed] [Google Scholar]
  9. Gois M., Sisak F., Kuksa F., Sovadina M. Incidence and evaluation of the microbial flora in the lungs of pigs with enzootic pneumonia. Zentralblatt für Veterinärmedizin, B. 1975;22:205–219. doi: 10.1111/j.1439-0450.1975.tb00581.x. [DOI] [PubMed] [Google Scholar]
  10. Halbur P.G., Paul P.S., Vaughn E.M., Andrews J.J. Experimental reproduction of pneumonia in gnotobiotic pigs with porcine respiratory coronavirus isolate AR310. Journal of Veterinary Diagnostic Investigation. 1993;5:184–188. doi: 10.1177/104063879300500207. [DOI] [PubMed] [Google Scholar]
  11. Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T., Williams S.T. Facultatively anaerobic gram negative rods. In: Hensyl W.R., editor. Bergey's Manual of Determinative Bacteriology. 9th Edit. Williams and Wilkins Press; Baltimore, Maryland: 1994. pp. 195–289. [Google Scholar]
  12. Hopper S.A., White M.E.C., Twiddy N. An outbreak of blue-eared disease (porcine reproductive and respiratory syndrome) in four pig herds in Great Britain. Veterinary Record. 1992;131:140–144. doi: 10.1136/vr.131.7.140. [DOI] [PubMed] [Google Scholar]
  13. Kay R.M., Done S.H., Paton D.J. Effect of sequential porcine reproductive and respiratory syndrome and swine influenza on the growth and performance of finishing pigs. Veterinary Record. 1994;135:199–204. doi: 10.1136/vr.135.9.199. [DOI] [PubMed] [Google Scholar]
  14. L'Ecuyer C., Switzer W.P., Roberts E.D. Microbiologic survey of pneumonic and normal swine lungs. American Journal of Veterinary Research. 1961;22:1020–1025. [PubMed] [Google Scholar]
  15. Murakami Y., Kato A., Tsuda T., Morozumi T., Miura Y., Sugimura T. Isolation and serological characterization of porcine reproductive and respiratory syndrome (PRRS) viruses from pigs with reproductive and respiratory disorders in Japan. Journal of Veterinary Medical Science. 1994;56:891–894. doi: 10.1292/jvms.56.891. [DOI] [PubMed] [Google Scholar]
  16. Rossow K.D., Bautista E.M., Goyal S.M., Moltor T.W., Murtaugh M.P., Morrison R.B., Benfield D.A., Collins J.E. Experimental porcine reproductive and respiratory syndrome virus infection in one-, four-, and 10-week-old pigs. Journal of Veterinary Diagnostic Investigation. 1994;6:3–12. doi: 10.1177/104063879400600102. [DOI] [PubMed] [Google Scholar]
  17. Shimizu M., Yamada S., Murakami Y., Morozumi T., Kobayashi H., Mitani K., Ito N., Kubo M., Kimura K., Kobayashi M., Yamamoto K., Miura Y., Yamamoto T., Watanabe K. Isolation of porcine reproductive and respiratory syndrome (PRRS) virus from Heko-Heko disease of pigs. Journal of Veterinary Medical Science. 1994;56:389–391. doi: 10.1292/jvms.56.389. [DOI] [PubMed] [Google Scholar]
  18. Stevenson G.W., van Alstine W.G., Kanitz C.L., Keffaber K.K. Endemic porcine reproductive and respiratory syndrome virus infection of nursery pigs in two swine herds without current reproductive failure. Journal of Veterinary Diagnostic Investigation. 1993;5:432–434. doi: 10.1177/104063879300500322. [DOI] [PubMed] [Google Scholar]
  19. Terpstra C., Wensvoort G., Pol J.M.A. Experimental reproduction of porcine epidemic abortion and respiratory syndrome (mystery swine disease) by infection with Lelystad virus: Koch's postulates fulfilled. Veterinary Quarterly. 1991;13:131–136. doi: 10.1080/01652176.1991.9694297. [DOI] [PubMed] [Google Scholar]
  20. Wensvoort G., Terpstra C., Pol J.M.A., Ter Laak E.A., Bloemraad M., De Kluyver E.P., Kragten C., Van Buiten L., Den Besten A., Wagenaar F., Broekhuijsen J.M., Moonen P.L.J.M., Zetstra T., De Boer E.A., Tibben H.J., De Jong M.F., Van't Veld P., Groenland G.J.R., Van Gennep J.A., Voets M.Th., Verheijden J.H.M., Braamskamp J. Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Veterinary Quarterly. 1991;13:121–130. doi: 10.1080/01652176.1991.9694296. [DOI] [PubMed] [Google Scholar]
  21. Yoon I.J., Joo H.S., Christianson W.T., Kim H.S., Collins J.E., Carlson J.H., Dee S.A. Isolation of a cytopathic virus from weak pigs on farms with a history of swine infertility and respiratory syndrome. Journal of Veterinary Diagnostic Investigation. 1992;4:139–143. doi: 10.1177/104063879200400204. [DOI] [PubMed] [Google Scholar]

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