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. 2016 Jun 10;101(4):895–901. doi: 10.1016/0016-5085(91)90713-U

Electrolyte transport in piglets infected with transmissible gastroenteritis virus 

Stimulation by verapamil and clonidine

Fadia R Homaidan a,b, Alfonso Torres a,b,1, Mark Donowitz a,b, Geoffrey WG Sharp a,b
PMCID: PMC7130406  PMID: 1889713

Abstract

The effects of clonidine, an α2-adrenergic agonist, and verapamil, a Ca2+ channel blocker, on Na2+ and Cl− absorption were studied in stripped jejunal mucosa from control and transmissible-gastroenteritis-virus-infected piglets. All infected piglets developed severe diarrhea 18–24 hours after oral inoculation. Jejunum from infected animals, as compared with control jejunum, had decreased mucosal-to-serosal, serosal-to-mucosal, and net Na+ and Cl− fluxes. Clonidine and verapamil caused a decrease in short-circuit current and stimulation of Na+ and Cl− absorption in control jejunum. In infected piglets, although the jejunum exhibited severe villus atrophy, both drugs stimulated Na+ and Cl− absorption and the magnitude of Na+ and Cl− absorption was similar in control and transmissible-gastroenteritis-infected jejunum. In contrast, d-glucose stimulated Na+ absorption, and the decrease in shortcircuit current caused by verapamil and clonidine, were decreased in transmissible-gastroenteritis-infected jejunum. Such pharmacological stimulation of Na+ and Cl− absorption might be useful in the management and treatment of certain viral diarrheal diseases.

Abbreviations: [Ca2+]i, intracellular Ca2+ concentration; G, conductance; Isc, short-circuit current; PD, potential difference; TGE, transmissible gastroenteritis

Footnotes

 

Supported by a National Institutes of Health Biomedical Research Support Grant and by National Institutes of Health grants R01-DK-26523, R01-DK-31667 and Research Career Development Award 1KOH-00588.

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