Skip to main content
NCBI home page
Elsevier - PMC COVID-19 Collection logoLink to Elsevier - PMC COVID-19 Collection
. 2006 Jun 3;113(1):59–67. doi: 10.1016/S0021-9975(05)80069-6

An immunohistochemical investigation of porcine epidemic diarrhoea

M Sueyoshi 1, T Tsuda 1, K Yamazaki *, K Yoshida 1, M Nakazawa 1, K Sato 1, T Minami 1, K Iwashita †, M Watanabe †, Y Suzuki ‡, M Mori ‡
PMCID: PMC7130422  PMID: 7490338

Summary

A sudden outbreak of epidemic diarrhoea of piglets occurred in Japan, the principal features being watery diarrhoea, dehydration and high mortality in newborn animals. The microscopical lesions were villous atrophy in the small intestine, the villous enterocytes being vacuolated and cuboidal in shape. The villus-crypt ratio was severely reduced, varying from 1:1 to 3:1. Transmission electron microscopy showed numerous coronaviruses within the cytoplasm of enterocytes and among microvilli. Specific antigens of porcine epidemic diarrhoea (PED) virus were detected in the cytoplasm of enterocytes by the streptavidin-biotin (SAB) technique. Infected cells, which were most abundant in the villous epithelia of the jejunum and ileum, were present in small numbers in the large intestine, the crypt epithelia, the lamina propria and Peyer's patches. The study suggests that the SAB technique is useful for the diagnosis of PED.

References

  1. Chasey D., Cartwright S.F. Virus-like particles associated with porcine epidemic diarrhoea. Research in Veterinary Science. 1978;25:255–256. doi: 10.1016/S0034-5288(18)32994-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cox E., Hooyberghs J., Pensaert M.B. Sites of replication of a porcine respiratory coronavirus related to transmissible gastroenteritis virus. Research in Veterinary Science. 1990;48:165–169. doi: 10.1016/S0034-5288(18)30984-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Debouck P., Pensaert M. Experimental infection of pigs with a new porcine enteric coronavirus CV 777. American Journal of Veterinary Research. 1980;41:219–223. [PubMed] [Google Scholar]
  4. Debouck P., Pensaert M., Coussement W. The pathogenesis of an enteric infection in pigs, experimentally induced by the coronavirus-like agent, CV 777. Veterinary Microbiology. 1981;6:157–165. [Google Scholar]
  5. Ducatelle R., Coussement W., Charlier G., Debouck P., Hoorens J. Three-dimensional sequential study of the intestinal surface in experimental porcine CV 777 coronavirus enteritis. Zentralblatt für Veterinärmedizin, B. 1981;28:483–493. doi: 10.1111/j.1439-0450.1981.tb01765.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hooper B.E., Haelterman E.O. Growth of transmissible gastroenteritis virus in young pigs. American Journal of Veterinary Research. 1966;27:286–291. [PubMed] [Google Scholar]
  7. Horvath I., Mocsari E. Ultrastructural changes in the small intestinal epithelium of suckling pigs affected with a transmissible gastroenteritis (TGE)-like disease. Archives of Virology. 1981;68:103–113. doi: 10.1007/BF01314440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kusanagi K., Kuwahara H., Katoh T., Nunoya T., Ishikawa Y., Samejima T., Tajima M. Isolation and serial propagation of porcine epidemic diarrhoea virus in cell cultures and partial characterization of the isolate. Journal of Veterinary Medical Science. 1992;54:313–318. doi: 10.1292/jvms.54.313. [DOI] [PubMed] [Google Scholar]
  9. Kuwahara H., Nunoya T., Samejima T., Tajima M. Passage in piglets of a coronavirus associated with porcine epidemic diarrhoea. Journal of the Japan Veterinary Medical Association. 1988;41:169–173. [Google Scholar]
  10. Pensaert M.B., Debouck P. A new coronavirus-like particle associated with diarrhoea in swine. Archives of Virology. 1978;58:243–247. doi: 10.1007/BF01317606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pensaert M.B., Debouck P., Reynolds D.J. An immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, CV 777, and several coronaviruses. Archives of Virology. 1981;68:45–52. doi: 10.1007/BF01315166. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pospischil A., Hess R.G., Bachmann P.A. Light microscopy and ultrahistology of intestinal changes in pigs infected with epizootic diarrhoea virus (EVD): comparison with transmissible gastroenteritis (TGE) virus and porcine rotavirus infections. Zentralblatt für Veterinärmedizin, B. 1981;28:564–577. doi: 10.1111/j.1439-0450.1981.tb01774.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Takahashi K., Okada K., Ohshima K. An outbreak of swine diarrhoea of a new type associated with coronavirus-like particles in Japan. Japan Journal of Veterinary Science. 1983;45:829–832. doi: 10.1292/jvms1939.45.829. [DOI] [PubMed] [Google Scholar]
  14. Turgeon D.C., Morin M., Jolette J., Higgins R., Marsolais G., Difranco E. Coronavirus-like particles associated with diarrhea in baby pigs in Quebec. Canadian Veterinary Journal. 1980;21:100–101. [PMC free article] [PubMed] [Google Scholar]
  15. Wood E.N. An apparently new syndrome of porcine epidemic diarrhoea. Veterinary Record. 1977;100:243–244. doi: 10.1136/vr.100.12.243. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Comparative Pathology are provided here courtesy of Elsevier

RESOURCES