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. 2002 Nov 12;127(2):187–195. doi: 10.1016/0022-1759(90)90068-7

Monoclonal antibodies from Epstein-Barr virus-transformed lymphocytes of common marmosets (Callithrix jacchus) immune to malaria

DA Johnston 1,2, AM Knight 1, BA Naylor 2, N Wedderburn 2, GH Mitchell 1,∗
PMCID: PMC7130502  PMID: 2155967

Abstract

The B lymphocytes of the common marmoset Callithrix jacchus can be immortalised by infection with Epstein-Barr virus (EBV) in vitro (Desranges et al., 1976). C. jacchus is susceptible to infection with the blood stages of several species of malaria parasite including the line designated MVF1 (Mitchell et al., 1988) from which it recovers and shows immunity to reinfection. By exploiting these two phenomena, EBV-transformed, marmoset lymphoblastoid cell lines secreting antibodies to malaria parasite antigens have been generated and cloned. We believe this to be the first time that monoclonal antibodies (MAbs) have been raised from common marmosets. Since numerous and diverse human pathogens can infect this small primate in the laboratory, these methods may prove generally applicable for the generation of MAbs whose specificities derive from immune responses to infection.

Keywords: Common marmoset, Epstein-Barr virus, Feeder cell, Malaria, Monoclonal antibody

Abbreviations: DMSO, dimethyl sulphoxide; EBV, Epstein-Barr virus; EDTA, ethylenediaminetetraacetic acid; EGTA, 1,2-di(2-aminoethoxy)ethane-N,N,N′,N′-tetraacetic acid; FCS, heat-inactevated foetal calf serum; FITC, fluorescein iso-thiocyanate; Ig, immunoglobulin; LCL, lymphoblastoid cell line; Mab, monoclonal antibody; ND, not done; NP40, Nonidet P-40; PBL, peripheral blood lymphocyte; PI, post infection; PMSF, phenylmethylsulfonyl fluoride; PRO, 2,5-diphenyloxazole; PT, post transformation; RBC, red blood cell; SDS-PAGE, sodium dodecyl sulphate-polyacrylamide gel electrophoresis; T, transformation; TES, N-Tris(hydroxymethyl)methyl-2amino-ethanesulphonic acid; TLCK, Nα-tosyl-L-lysyl chloromethane hydrochloride; Tris, tris(hydroxymethyl)methylamine

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