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. 2002 Nov 12;144(1):1–10. doi: 10.1016/0022-1759(91)90223-3

CD4 rat × rat and mouse × rat T cell hybridomas produced by fusion of established T cell lines and clones to W/Fu (C58NT)D

AMH Boots 1,2, MJ van Lierop 2, MHM Wauben 2, PJS van Kooten 2, EJ Hensen 2, W van Eden 2,∗
PMCID: PMC7130835  PMID: 1960397

Abstract

Previously, fusion of established T cell lines or clones has been claimed to be difficult. We now report our experiences in the fusion of both long term cultures of rat T cell clones and mouse T cell lines to rat W/Fu (C58NT)D. Upon fusion of rat T cell clones the hybrids obtained expressed antigen specificities identical to those of the parent clones. In addition, C58 was used for interspecies hybridisation of murine T cell lines. The specificity of intra- and inter-species hybrids was maintained by subcloning.

We conclude that the C58 cell line can be used to generate continuously growing monoclonal T-cell reagents of sufficient stability using both intra- and inter-species hybri disation.

Keywords: CD4+, T cell hybridoma, Intra- and inter-species hybridisation

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