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. 2004 Feb 10;40(4):1030–1038. doi: 10.1016/0042-6822(70)90149-2

Electron microscopic observations of visna virus-infected cell cultures☆

Joe E Coward 1, Donald H Harter 1,2, Councilman Morgan 1
PMCID: PMC7130857  PMID: 4194173

Abstract

Electron microscopic observations of three cell lines infected with visna virus revealed two types of extracellular particles. The smaller of these was 65–110 mμ in diameter and contained a 20–30 mμ electron-dense core. Ordered arrays of the latter type of particle occurred rarely in the cytoplasm. After cesium chloride density gradient centrifugation of the virus, the band that contained maximal infectivity was composed of numerous particles with osmiophilic cores similar to those found in infected cell cultures. This finding suggests that such particles represent the infective agent. The second type of extracellular particle was larger (100–140 mμ in diameter), lacked an electron-dense core, and contained material similar in appearance to cellular cytoplasm. This form appeared to develop by budding from the cell surface.

Footnotes

☆

Supported by Public Health Service Grants NB 06989 from the National Institute of Neurological Diseases and AI 06814 from the National Institute of Allergy and Infectious Diseases, Grant GB 6013 from the National Science Foundation, research contract No. DADA 17-67-C-7141 from the U. S. Army Medical Research and Development Command, Department of the Army, and a gift from the Miles Hodson Vernon Foundation, Inc.

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