Skip to main content
PMC home page
Elsevier - PMC COVID-19 Collection logoLink to Elsevier - PMC COVID-19 Collection
. 2004 Feb 6;174(2):605–607. doi: 10.1016/0042-6822(90)90114-7

Mouse hepatitis virus ORF 2a is expressed in the cytosol of infected mouse fibroblasts

Philip W Zoltick ∗, Julian L Leibowitz †, Emilia L Oleszak †, Susan R Weiss ∗,1
PMCID: PMC7131050  PMID: 2154893

Abstract

A 266-bp fragment of cDNA from within gene B, ORF 2a, of MHV-A59 was used to construct a vector encoding a bacterial/viral fusion protein. Antiserum raised against this fusion protein specifically immunoprecipitates a 30K protein from infected 17CI-1 mouse fibroblasts. The protein is localized primarily in the cytosol and not in the membranes. This is consistent with its predicted sequence and potential role as an RNA binding protein.

References

  • 1.Luytjes W., Bredenbeek P.J., Noten A.F.S., Horzinek M.C., Spaan W.J.M. Virology. 1988;166:415–422. doi: 10.1016/0042-6822(88)90512-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Spaan W.J.M., Cavanagh D., Horzinek M.C. J. Gen. Virol. 1988;69:2939–2952. doi: 10.1099/0022-1317-69-12-2939. [DOI] [PubMed] [Google Scholar]
  • 3.Pachuk C.J., Bredenbeek P.J., Zoltick P.W., Spaan W.J.M., Weiss S.R. Virology. 1989;171:141–148. doi: 10.1016/0042-6822(89)90520-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Skinner M.A., Siddell S.G. J. Gen. Virol. 1985;66:593–596. doi: 10.1099/0022-1317-66-3-593. [DOI] [PubMed] [Google Scholar]
  • 5.Skinner M.A., Ebner D., Siddell S.G. J. Gen. Virol. 1985;66:581–592. doi: 10.1099/0022-1317-66-3-581. [DOI] [PubMed] [Google Scholar]
  • 6.Budzilowicz C.J., Weiss S.R. Virology. 1987;157:509–515. doi: 10.1016/0042-6822(87)90293-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Leibowitz J.L., DeVries J.R., Haspel M.V. J. Virol. 1982;42:1080–1087. doi: 10.1128/jvi.42.3.1080-1087.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Leibowitz J.L., Perlman S., Weinstock G., DeVries J.R., Budzilowicz C.J., Weissmann J.M., Weiss S.R. Virology. 1988;164:156–164. doi: 10.1016/0042-6822(88)90631-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Ebner D., Siddell S.G. In: Advances in Experimental Medicine and Biology. Lai M.M.C., Stohlman S.A., editors. Plenum Press; New York: 1986. pp. 39–45. [Google Scholar]
  • 10.Leibowitz J.L., Weiss S.R., Paavola E., Bond C.W. J. Virol. 1982;43:905–913. doi: 10.1128/jvi.43.3.905-913.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Zoltick P.W., Leibowitz J.L., DeVries J.R., Weinstock G., Weiss S.R. Gene. 1989 doi: 10.1016/0378-1119(89)90434-4. in press. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Wilhelmson K.C., Leibowitz J.L., Bond C.W., Robb J.A. Virology. 1981;110:225–230. doi: 10.1016/0042-6822(81)90027-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Leibowitz J.L., Wilhelmson D.C., Bond C.W. Virology. 1981;114:39–51. doi: 10.1016/0042-6822(81)90250-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Maizel J.V., Jr. In: Mamarosch K., Keprowski H., editors. Vol. 5. Academic Press; New York: 1971. pp. 179–246. (Virology). [Google Scholar]
  • 15.Brayton P.R., Lai M.M.C., Patton C.D., Stohlman S.A. J. Virol. 1982;42:847–853. doi: 10.1128/jvi.42.3.847-853.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Virology are provided here courtesy of Elsevier

RESOURCES