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. 2004 Feb 23;137(2):297–304. doi: 10.1016/0042-6822(84)90221-6

Characterization of murine hepatitis virus (JHM) RNA from rats with experimental encephalomyelitis

Dennis P Jackson ∗, Dean H Percy †, Vincent L Morris ∗,1
PMCID: PMC7131065  PMID: 6091331

Abstract

When Wistar Furth rats are inoculated intracerebrally with the murine hepatitis virus JHM they often develop a demyelinating disease with resulting hind leg paralysis. Using an RNA transfer procedure and hybridization kinetic analysis, the virus-specific RNA in these rats was characterized. The pattern of JHM-specific RNA varied with individual infections of Wistar Furth rats. However, two species of JHM-specific RNA, the nucleocapsid and a 2.1–2.4 × 106-Da RNA species were generally present. A general decrease in JHM-specific RNA in brains' and spinal cord samples taken later than 20 days postinoculation was observed; however, JHM-specific RNA persisted in the spinal cord longer than in the brain of these rats.

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