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. 2004 Feb 11;191(1):368–386. doi: 10.1016/0042-6822(92)90199-Y

Rearrangement of viral sequences in cytopathogenic pestiviruses☆

Gregor Meyers a,†,∗,‡, Norbert Tautz a,†,∗,‡, Robert Stark a,†,∗,‡, Joe Brownlie ∗, Edward J Dubovi †, Marc S Collett ‡, Heinz-Jürgen Thiel a,†,∗,‡,1
PMCID: PMC7131167  PMID: 1329326

Abstract

Two cytopathogenic isolates of bovine viral diarrhea virus (cpBVDV) have been analyzed. For both viruses two regions of their genomic RNAs were found to be duplicated and rearranged. The viral genomes contain a small duplicated element (SD) derived from the genomic 5' end far downstream of its original context. This sequence is followed by a larger duplication which encompasses the region coding for the protein p80(LD), a molecular marker for cpBVDV. The SD element codes for the viral protease p20. In the case of the viruses analyzed here the aminoterminus of p80 is generated by autoproteolytic removal of the preceding SD-encoded protease. For one of the cpBVDV isolates a specific fusion protein (p28) could be identified which is composed of p20 and part of p10, another viral protein. Molecular characterization of the respective noncytopathogenic counterpart revealed that duplication and rearrangement of sequences as well as the expression of p28 and p80 are specific for the cytopathogenic virus.

Footnotes

☆

Sequence data from this article have been deposited with GenBank/EMBL Data Libraries under accession Nos. M96640 (Fig. 3A), M96641 (Fig. 3B), M96638 (Fig. 3C), and M96639 (Fig. 4).

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