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. 2004 Jun 9;112(2):596–604. doi: 10.1016/0042-6822(81)90305-6

Intracellular murine hepatitis virus-specific RNAs contain common sequences

Steve Cheley 1, Robert Anderson 1, Margaret J Cupples 1, Edwin CM Lee Chan 1, Vincent L Morris 1,1
PMCID: PMC7131256  PMID: 6114592

Abstract

A major polyadenylated viral RNA of approximately 0.8 × 106 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded 32P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 106 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.

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