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. 2012 Feb 20;98(3):323–331. doi: 10.1016/S0065-1281(96)80025-0

Immunoelectron microscopic single and double labelling of aminopeptidase N (CD 13) and dipeptidyl peptidase IV (CD 26)

Thoralf Stange 1, Ursula Kettmann 2, Hans-Jürgen Holzhausen 3,1
PMCID: PMC7131626  PMID: 8863861

Summary

Using ultrathin cryosections and immunogold labelling, aminopeptidase N (CD 13) and dipeptidyl peptidase IV (CD 26) were localized on the luminal side of the brush border membrane of proximal tubular cells in human kidney as well as of enterocytes from rat small intestine. Furthermore, both enzymes could be detected on the cell surface of human T lymphocytes and especially aminopeptidase N on human synovial fibroblasts. Gold labelled vesicular structures were also found in the cytoplasm in the apical part of renal proximal tubular cells and synovial fibroblasts. In human kidney the colocalization of the two membrane antigens was possible by using several double labelling methods.

Key words: aminopeptidase N, CD 13, dipeptidyl peptidase IV, CD 26, cell surface antigens, immunoelectron microscopy, cryoultramicrotomy, immunogold labelling, double labelling

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