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. 2008 Feb 28;16:357–396. doi: 10.1016/S0065-3527(08)60027-5

Australia (Hepatitis-Associated) Antigen: Physicochemical And Immunological Characteristics*

George L Le Bouvier 1, Robert W McCollum 1
PMCID: PMC7131679  PMID: 4993220

Publisher Summary

This chapter discusses the discovery, characterization, immunology, and etiological significance of “Australia antigen (Au),” a new antigenic specificity appearing in the serum of patients with serum hepatitis, and carried on characteristic lipoprotein particles. In addition to its original name, given before its relationship to viral hepatitis became known, the antigen has also been given the designations, including Au(1) , SH antigen, Au/SH antigen, hepatitis antigen, and hepatitis-associated antigen (HAA). The synthesis of Au antigen, and its appearance in the serum, is specifically associated with infection by the causal agent of the SH type of viral hepatitis. The chapter discusses properties of the antigen, and of the particles which carry the Au specificity. It also discusses various serological techniques for Au antigen, such as two-dimensional double immunodiffusion (ID), complement fixation (CF), immunofluorescence (IF), reversed passive hemagglutination (RPHA) of antibody-coated red cells, immune electron microscopy (IEM), immunoelectroosmophoresis (IEOP), and radioimmunoassay (RIA).

References

  1. Agarwal S.S., Blumberg B.S., Gerstley B.J.S., Sutnick A.I., London W.T., Loeb L.A. Clin. Res. 1970;18:436. doi: 10.1172/JCI106215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Allison A.C., Blumberg B.S. Lancet. 1961;i:634–637. doi: 10.1016/s0140-6736(61)91654-3. [DOI] [PubMed] [Google Scholar]
  3. Almeida J.D., Waterson A.P. Lancet. 1969;ii:983–986. doi: 10.1016/s0140-6736(69)90540-6. [DOI] [PubMed] [Google Scholar]
  4. Almeida J.D., Zuckerman A.J., Taylor P.E., Waterson A.P. Microbios. 1969;2:117–123. [Google Scholar]
  5. Alter H.J., Blumberg B.S. Blood. 1966;27:297–309. [PubMed] [Google Scholar]
  6. Bang F.B. New Engl. J. Med. 1969;281:1190–1191. doi: 10.1056/NEJM196911202812111. [DOI] [PubMed] [Google Scholar]
  7. Barker L.F., Smith K.O., Gehle W.D., Shulman N.R. J. Immunol. 1969;102:1529–1532. [PubMed] [Google Scholar]
  8. Barker L.F., Shulman N.R., Murray R., Hirschman R.J., Ratner F., Diefenbach W.C.L., Geller H.M. J. Amer. Med. Ass. 1970;211:1509–1512. [PubMed] [Google Scholar]
  9. Bayer M.E., Blumberg B.S., Werner B. Nature (London) 1968;218:1057–1059. doi: 10.1038/2181057a0. [DOI] [PubMed] [Google Scholar]
  10. Berson S.A., Yalow R.S., Bauman A., Rothschild M.A., Newerly K. J. Clin. Invest. 1956;35:170–190. doi: 10.1172/JCI103262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Blumberg B.S. Bull. N.Y. Acad. Med. 1964;40:377–386. [PMC free article] [PubMed] [Google Scholar]
  12. Blumberg B.S. Perspect. Virol. 1970 in press. [Google Scholar]
  13. Blumberg B.S., Bernanke D., Allison A.C. J. Clin. Invest. 1962;41:1936–1944. doi: 10.1172/JCI104651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Blumberg B.S., Alter H.J., Visnich S. J. Amer. Med. Ass. 1965;191:541–546. doi: 10.1001/jama.1965.03080070025007. [DOI] [PubMed] [Google Scholar]
  15. Blumberg B.S., Melartin L., Guinto R.A., Werner B. Amer. J. Hum. Genet. 1966;18:594–608. [PMC free article] [PubMed] [Google Scholar]
  16. Blumberg B.S., Gerstley B.J.S., Hungerford D.A., London W.T., Sutnick A.I. Ann. Intern. Med. 1967;66:924–931. doi: 10.7326/0003-4819-66-5-924. [DOI] [PubMed] [Google Scholar]
  17. Blumberg B.S., Melartin L., Lechat M., Guinto R.A. Lancet. 1967;ii:173–176. doi: 10.1016/s0140-6736(67)90003-7. [DOI] [PubMed] [Google Scholar]
  18. Blumberg B.S., Sutnick A.I., London W.T. Bull. N.Y. Acad. Med. 1968;44:1566–1568. [PMC free article] [PubMed] [Google Scholar]
  19. Blumberg B.S., Friedlaender J.S., Woodside A., Sutnick A.I., London W.T. Proc. Nat. Acad. Sci. U.S. 1969;62:1108–1115. doi: 10.1073/pnas.62.4.1108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Coller J.A., Millman I. J. Clin. Invest. 1970;49:20a. [Google Scholar]
  21. Cossart Y.E., Field A.M. Lancet. 1970;i:695–698. doi: 10.1016/s0140-6736(70)92460-8. [DOI] [PubMed] [Google Scholar]
  22. Cowan K.M., Graves J.H. Virology. 1968;34:544–548. [PubMed] [Google Scholar]
  23. Coyne V.E., Millman I., Cerda J., Gerstley B.J.S., London W.T., Sutnick A.I., Blumberg B.S. J. Exp. Med. 1970;131:307–319. doi: 10.1084/jem.131.2.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Dane D.S., Cameron C.H., Briggs M. Lancet. 1970;i:695–698. doi: 10.1016/s0140-6736(70)90926-8. [DOI] [PubMed] [Google Scholar]
  25. Deinhardt, F. Holmes, A. W. Harris, W. Cline, J.B. Ball, F. L. 1970 Unpublished observations.
  26. Dixon F.J. Harvey Lect. 1963;58:21–52. [PubMed] [Google Scholar]
  27. Fox R.A., Niazi S.P., Sherlock S. Lancet. 1969;ii:609–612. doi: 10.1016/s0140-6736(69)90324-9. [DOI] [PubMed] [Google Scholar]
  28. Gerin J.L., Purcell R.H., Hoggan M.D., Holl P.V., Chanock R.M. J. Virol. 1969;4:763–768. doi: 10.1128/jvi.4.5.763-768.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Gibbons R.A., Hunter G.D. Nature (London) 1967;215:1041–1043. doi: 10.1038/2151041a0. [DOI] [PubMed] [Google Scholar]
  30. Giles J.P., McCollum R.W., Berndtson L.W., Jr., Krugman S. New Engl. J. Med. 1969;281:119–122. doi: 10.1056/NEJM196907172810302. [DOI] [PubMed] [Google Scholar]
  31. Gitnick G.L., Gleich G.J., Schoenfield L.J., Baggenstoss A.H., Sutnick A.I., Blumberg B.S., London W.T., Summerskill W.H.J. Lancet. 1969;ii:285–288. doi: 10.1016/s0140-6736(69)90052-x. [DOI] [PubMed] [Google Scholar]
  32. Gocke D.J., Kavey N.B. Lancet. 1969;i:1055–1059. doi: 10.1016/s0140-6736(69)91701-2. [DOI] [PubMed] [Google Scholar]
  33. Gust I.D., Cross G., Kaldor J., Ferris A.A. Lancet. 1970;i:953. doi: 10.1016/s0140-6736(70)91082-2. [DOI] [PubMed] [Google Scholar]
  34. Hierholzerm, W.J. Jr. 1969 Unpublished data
  35. Hierholzer, W. J. Jr. Le Bouvier, G. L. 1969 Unpulished data.
  36. Hierholzer, W. J. Jr. Le Bouvier, G. L. Berndtson, L. W. Jr. 1970 Unpublished data
  37. Hirschman R.J., Shulman N.R., Barker L.F., Smith K.O. J. Amer. Med. Ass. 1969;208:1667–1670. [PubMed] [Google Scholar]
  38. Holl P.V., Walsh J.H., Morrow A.G., Purcell R.H. Lancet. 1969;ii:553–555. doi: 10.1016/s0140-6736(69)90259-1. [DOI] [PubMed] [Google Scholar]
  39. Hotchin J., Collins D. Nature (London) 1964;203:1357–1359. doi: 10.1038/2031357a0. [DOI] [PubMed] [Google Scholar]
  40. Juji T., Yokochi T. Jap. J. Exp. Med. 1969;39:83–88. [PubMed] [Google Scholar]
  41. Krugman, S. Giles, J. P. 1970 Personal communication
  42. Krugman S., Giles J.P., Hammond J. J. Amer. Med. Ass. 1967;200:365–373. doi: 10.1001/jama.200.5.365. [DOI] [PubMed] [Google Scholar]
  43. Le Bouvier G.L. Brit. J. Exp. Pathol. 1959;40:452–463. [PMC free article] [PubMed] [Google Scholar]
  44. Le Bouvier, G. L. 1969 Unpublished data.
  45. Le Bouvier, G. L. Hierholzer, W. J. Jr. 1969 Unpublished data.
  46. Le Bouvier, G. L. McCollum, R. W. Mirick, G. S. 1969 Unpublished observations
  47. Levene C., Blumberg B.S. Nature (London) 1969;221:195–196. doi: 10.1038/221195a0. [DOI] [PubMed] [Google Scholar]
  48. London W.T., Difiglia M., Sutnick A.I., Blumberg B.S. New Engl. J. Med. 1969;281:571–578. doi: 10.1056/NEJM196909112811101. [DOI] [PubMed] [Google Scholar]
  49. London W.T., Sutnick A.I., Blumberg B.S. Ann. Intern. Med. 1969;70:55–59. doi: 10.7326/0003-4819-70-1-55. [DOI] [PubMed] [Google Scholar]
  50. McCollum R.W. Proc. Soc. Exp. Biol. Med. 1952;81:157–160. doi: 10.3181/00379727-81-19809. [DOI] [PubMed] [Google Scholar]
  51. McCollum, R. W. 1970 Unpublished data
  52. Mathews J.D., Mackay I.R. Brit. Med J. 1970;i:259–261. doi: 10.1136/bmj.1.5691.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  53. Melartin L., Blumberg B.S. Nature (London) 1966;210:1340–1341. doi: 10.1038/2101340a0. [DOI] [PubMed] [Google Scholar]
  54. Mella B., Lang D.J. Science. 1967;155:80–81. doi: 10.1126/science.155.3758.80. [DOI] [PubMed] [Google Scholar]
  55. Melnick, J. L. 1969 Personal communication
  56. D.A. Merrill, P.F. Kohler, J.W. Cingleton. 1970 26th Annu. Meet. Amer. Acad. Allergy Progr. Abstr. No. 28. 42
  57. Millman I., Zavatone V., Gerstley B.J.S., Blumberg B.S. Nature (London) 1969;222:181–184. doi: 10.1038/222181b0. [DOI] [PubMed] [Google Scholar]
  58. Millman I., Loeb L.A., Bayer M.E., Blumberg B.S. J. Exp. Med. 1970;131:1190–1199. doi: 10.1084/jem.131.6.1190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  59. Millman I., Ziegenfuss J.F., Raunio V., London W.T., Sutnick A.I., Blumberg B.S. Proc. Soc. Exp. Biol. Med. 1970;133:1426–1431. doi: 10.3181/00379727-133-34705. [DOI] [PubMed] [Google Scholar]
  60. Millman I., London W.T., Sutnick A.I., Blumberg B.S. Nature (London) 1970;226:83–84. doi: 10.1038/226083a0. [DOI] [PubMed] [Google Scholar]
  61. Mosley J.W., Barker L.F., Shulman N.R., Hatch M.H. Nature (London) 1970;225:953–955. doi: 10.1038/225953a0. [DOI] [PubMed] [Google Scholar]
  62. Neefe J.R., Stokes J., Jr. J. Amer. Med. Ass. 1945;128:1063–1075. [Google Scholar]
  63. Nowoslawski A., Brzosko W.J., Madalinski K, Krawczynski K. Lancet. 1970;i:494–498. doi: 10.1016/s0140-6736(70)91580-1. [DOI] [PubMed] [Google Scholar]
  64. Okochi K., Murakami S. Vox Sang. 1968;15:374–385. doi: 10.1111/j.1423-0410.1968.tb04078.x. [DOI] [PubMed] [Google Scholar]
  65. Oldstone M.B.A., Dixon F.J. J. Exp. Med. 1969;129:483–505. doi: 10.1084/jem.129.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  66. Ouchterlony Ö. Progr. Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  67. Preer J.R., Jr., Preer L.B. J. Protozool. 1959;6:88–100. [Google Scholar]
  68. Prince A.M. Proc. Nat. Acad. Sci. U.S. 1968;60:814–821. doi: 10.1073/pnas.60.3.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  69. Prince A.M. Lancet. 1968;ii:642–643. [Google Scholar]
  70. Prince A.M. Perspect. Virol. 1970 in press. [Google Scholar]
  71. Prince A.M., Burke K. Science. 1970;169:593–595. doi: 10.1126/science.169.3945.593. [DOI] [PubMed] [Google Scholar]
  72. Prince A.M., Hargrove R.L., Szmuness M.D., Cherubin C.E., Fontana V.J., Jeffries G.H. New Engl. J. Med. 1970;282:987–991. doi: 10.1056/NEJM197004302821801. [DOI] [PubMed] [Google Scholar]
  73. Purcell, R. H. 1970 Personal communication
  74. Purcell R.H., Holl P.V., Walsh J.H., Wong D.C., Morrow A.G., Chanock R.M. J. Infec. Dis. 1969;120:383–386. doi: 10.1093/infdis/120.3.383. [DOI] [PubMed] [Google Scholar]
  75. Purcell R.H., Gerin J.L., Holl P.V., Cline W.L., Chanock R.M. J. Infec. Dis. 1970;121:222–226. doi: 10.1093/infdis/121.2.222. [DOI] [PubMed] [Google Scholar]
  76. Reinicke V., Nordenfelt E. Lancet. 1970;i:141–142. doi: 10.1016/s0140-6736(70)90491-5. [DOI] [PubMed] [Google Scholar]
  77. Shulman N.R., Barker L.F. Science. 1969;165:304–306. doi: 10.1126/science.165.3890.304. [DOI] [PubMed] [Google Scholar]
  78. Sutnick A.I., London W.T., Gerstley B.J.S., Cronlund M.M., Blumberg B.S. J. Amer. Med. Ass. 1968;205:670–674. [PubMed] [Google Scholar]
  79. Turner G.C., White G.B. Lancet. 1969;ii:121–124. [PubMed] [Google Scholar]
  80. Van Regenmortel M.H.V. Advan. Virus Res. 1966;12:207–271. doi: 10.1016/s0065-3527(08)60850-7. [DOI] [PubMed] [Google Scholar]
  81. Velasco M., Katz R. Lancet. 1970;i:779. doi: 10.1016/s0140-6736(70)91009-3. [DOI] [PubMed] [Google Scholar]
  82. Volkert M., Larsen J.H. Progr. Med. Virol. 1965;7:160–207. [PubMed] [Google Scholar]
  83. Walsh J.H., Yalow R.S., Berson S.A. J. Infec. Dis. 1970;121:550–554. doi: 10.1093/infdis/121.5.550. [DOI] [PubMed] [Google Scholar]
  84. Willems F.T.C., Melnick J.L., Rawls W.E. Proc. Soc. Exp. Biol. Med. 1969;130:652–661. doi: 10.3181/00379727-130-33628. [DOI] [PubMed] [Google Scholar]
  85. Wright R. Lancet. 1970;i:521–522. doi: 10.1016/S0140-6736(70)91605-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  86. Wright R., McCollum R.W., Klatskin G. Lancet. 1969;ii:117–121. doi: 10.1016/s0140-6736(69)92437-4. [DOI] [PubMed] [Google Scholar]
  87. Zuckerman A.J. Nature (London) 1969;223:569–572. doi: 10.1038/223569a0. [DOI] [PubMed] [Google Scholar]
  88. Zuckerman A.J., Taylor P.E. Nature (London) 1969;223:81–82. doi: 10.1038/223081a0. [DOI] [PubMed] [Google Scholar]
  89. Zuckerman A.J., Taylor P.E., Almeida J.D. Brit. Med. J. 1970;i:262–264. doi: 10.1136/bmj.1.5691.262. [DOI] [PMC free article] [PubMed] [Google Scholar]

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