MEDICAL SCIENCES Correction for “Mutations in thyroid hormone receptor α1 cause premature neurogenesis and progenitor cell depletion in human cortical development,” by Teresa G. Krieger, Carla M. Moran, Alberto Frangini, W. Edward Visser, Erik Schoenmakers, Francesco Muntoni, Chris A. Clark, David Gadian, Wui K. Chong, Adam Kuczynski, Mehul Dattani, Greta Lyons, Alexandra Efthymiadou, Faraneh Varga-Khadem, Benjamin D. Simons, Krishna Chatterjee, and Frederick J. Livesey, which was first published October 18, 2019; 10.1073/pnas.1908762116 (Proc. Natl. Acad. Sci. U.S.A. 116, 22754–22763).
The authors note that “In Fig. 4D, the horizontal line that highlights the comparison between neuronal activity in the three control lines and one of the three THRA mutant lines was drawn to end above the TRα1-FS382 mutation, rather than the TRα1-FS397 mutation. The placement of the horizontal line incorrectly indicated that neuronal activity in TRα1-FS382 mutation neurons was significantly lower than that of controls, whereas activity in TRα1-FS397 neurons was not. The figure has been amended, and now the horizontal line correctly indicates that neuronal activity in TRα1-FS397 neurons was significantly lower than that of controls, whereas activity in TRα1-FS382 neurons was not. We apologize for any confusion this labeling error may have caused.” The corrected Fig. 4 and its legend appear below.
Fig. 4.
FACS-enriched THRA mutation-containing cortical progenitor cells generate functional neuronal networks. (A) Representative FACS results for WT and THRA mutation-containing cells at day 18. Immunocytochemistry confirmed the cortical identity of FACS- selected progenitor cells. (Scale bars: 50 μm.) (B) Neurons produced by FACS-purified progenitor cells expressed the cortical layer-specific transcription factors TBR1 (layer VI) and CTIP2 (layer V), as well as the vesicular glutamate transporter vGlut1, at day 40. (Scale bars: 50 μm.) (C) Representative traces showing action potential firing in response to stepwise current injection in THRA mutation-containing and control neurons. Numbers indicate the proportion of cells that fired action potentials. (D) Calcium indicator Oregon Green BAPTA was used as a proxy for action potential firing to measure spontaneous neuronal activity. Fluorescence images show control and THRA mutant cultures after loading. Heat maps highlight different levels of activity across the cultures. Total calcium activity across the field of view, ACa, was quantified based on 3–6 videos from each cell line (403X and FS382, n = 6 samples from 2 independent inductions; all others, n = 3 samples from 1 induction; *P < 0.05, Student’s t test). Error bars indicate SEM.
The authors also note that the author name Faraneh Varga-Khadem should instead appear as Faraneh Vargha-Khadem. The corrected author line appears below. The online version has been corrected.
Teresa G. Krieger, Carla M. Moran, Alberto Frangini, W. Edward Visser, Erik Schoenmakers, Francesco Muntoni, Chris A. Clark, David Gadian, Wui K. Chong, Adam Kuczynski, Mehul Dattani, Greta Lyons, Alexandra Efthymiadou, Faraneh Vargha-Khadem, Benjamin D. Simons, Krishna Chatterjee, and Frederick J. Livesey