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. 2004 Apr 14;50(2):163–169. doi: 10.1016/0092-8674(87)90212-1

Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader

Beate Schwer 1, Paolo Visca 1, Jan C Vos 1, Hendrik G Stunnenberg 1
PMCID: PMC7133236  PMID: 3594569

Abstract

We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the “start site” determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5′ terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position −1, confirms the existence of a poly(A) leader of approximately 35 A-residues.

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