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. 2004 Apr 28;29(3):921–928. doi: 10.1016/0092-8674(82)90454-8

Recombination in RNA

Andrew MQ King 1, David McCahon 1, William R Slade 1, John WI Newman 1
PMCID: PMC7133258  PMID: 6295637

Abstract

The aphthovirus genome consists of a single molecule of single-stranded RNA that encodes all the virus-induced proteins. We isolated recombinant aphthoviruses from cells simultaneously infected with temperature-sensitive mutants of two different subtype strains. Analysis of the proteins induced by 16 independently generated recombinants revealed two types of protein pattern, which were consistent with single genetic crossovers on the 5′ side and 3′ side, respectively, of the central P34-coding region. Recombinants invariably inherited all four coat proteins from the same parent, and novel recombinant proteins were not observed. RNAase T1 fingerprints of virus RNA, prepared from representatives of each recombinant type, confirmed the approximate crossover sites that had been deduced from the inheritance of proteins. These fingerprints provide molecular evidence of recombination at the level of RNA and demonstrate the potential of RNA recombination for producing genetic diversity among picornaviruses.

References

  1. Cooper P.D. A genetic map of poliovirus temperature-sensitive mutants. Virology. 1968;35:584–596. doi: 10.1016/0042-6822(68)90287-0. [DOI] [PubMed] [Google Scholar]
  2. Cooper P.D. Genetics of picornaviruses. In: Fraenkel-Conrat H., Wagner R.R., editors. Vol. 9. Plenum Press; New York: 1977. pp. 133–207. (Comprehensive Virology). [Google Scholar]
  3. Fields S., Winter G. Nucleotide sequences of influenza virus segments 1 and 3 reveal mosaic structure of a small viral RNA segment. Cell. 1982;28:303–313. doi: 10.1016/0092-8674(82)90348-8. [DOI] [PubMed] [Google Scholar]
  4. Flanegan J.B., Baltimore D. Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polypeptide. J. Virol. 1979;29:352–360. doi: 10.1128/jvi.29.1.352-360.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harris T.J.R., Robson K.J.H., Brown F. A study of nucleotide sequence conservation between RNAs of two serotypes of foot-and-mouth disease virus. J. Gen. Virol. 1980;50:403–418. doi: 10.1099/0022-1317-50-2-403. [DOI] [PubMed] [Google Scholar]
  6. Hirst G.K. Vol. 27. 1962. Genetic recombination with Newcastle disease virus, polioviruses and influenza; pp. 303–308. (Cold Spring Harbor Symp. Quant. Biol.). [DOI] [PubMed] [Google Scholar]
  7. King A.M.Q., Newman J.W.I. Temperature-sensitive mutants of foot-and-mouth disease virus with altered structural polypeptides. I. Identification by electrofocusing. J. Virol. 1980;34:59–66. doi: 10.1128/jvi.34.1.59-66.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. King A.M.Q., Slade W.R., Newman J.W.I., McCahon D. Temperature-sensitive mutants of foot-and-mouth disease virus with altered structural polypeptides. II. Comparison of recombination and biochemical maps. J. Virol. 1980;34:67–72. doi: 10.1128/jvi.34.1.67-72.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. King A.M.Q., Underwood B.O., McCahon D., Newman J.W.I., Brown F. Biochemical identification of viruses causing the 1981 outbreaks of foot-and-mouth disease in the U. K. Nature. 1981;293:479–480. doi: 10.1038/293479a0. [DOI] [PubMed] [Google Scholar]
  10. King A.M.Q., McCahon D., Slade W.R., Newman J.W.I. Biochemical evidence of recombination within the unsegmented genome of aphthovirus. J. Virol. 1982;41:66–77. doi: 10.1128/jvi.41.1.66-77.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lai M.M.C., Brayton P.R., Armen R.C., Patton C.D., Pugh C., Stohlman S.A. Mouse hepatitis virus A59: mRNA structure and genetic localization of the sequence divergence from hepatotropic strain MHV-3. J. Virol. 1981;39:823–834. doi: 10.1128/jvi.39.3.823-834.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lake J.R., Priston R.A.J., Slade W.R. A genetic recombination map of foot-and-mouth disease virus. J. Gen. Virol. 1975;27:355–367. doi: 10.1099/0022-1317-27-3-355. [DOI] [PubMed] [Google Scholar]
  13. Lazzarini R.A., Keene J.D., Schubert M. The origins of defective interfering particles of the negative-strand RNA viruses. Cell. 1981;26:145–154. doi: 10.1016/0092-8674(81)90298-1. [DOI] [PubMed] [Google Scholar]
  14. Ledinko N. Studies of crosses between a normal horse serum-resistant mutant and several guanidine-resistant mutants of the same strain. Vol. 20. 1963. Genetic recombination with poliovirus type 1; pp. 107–119. (Virology). [DOI] [PubMed] [Google Scholar]
  15. Lehtovaara P., Soderlund H., Keranen S., Pettersson R.F., Kaariainen L. Vol. 78. 1981. 18S defective interfering RNA of Semliki Forest virus contains a triplicated linear repeat; pp. 5353–5357. (Proc. Nat. Acad. Sci. USA). [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lowe P.A., King A.M.Q., McCahon D., Brown F., Newman J.W.I. Vol. 78. 1981. Temperature-sensitive RNA polymerase mutants of a picornavirus; pp. 4448–4452. (Proc. Nat. Acad. Sci. USA). [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McCahon D. Brief review. Vol. 69. 1981. The genetics of aphthovirus; pp. 1–23. (Arch. Virol.). [Google Scholar]
  18. McCahon D., Slade W.R. A sensitive method for the detection and isolation of recombinants of foot-and-mouth disease virus. J. Gen. Virol. 1981;53:333–342. doi: 10.1099/0022-1317-53-2-333. [DOI] [PubMed] [Google Scholar]
  19. McCahon D., Slade W.R., Priston K.A.J., Lake J.R. An extended genetic recombination map for foot-and-mouth disease virus. J. Gen. Virol. 1977;35:555–565. doi: 10.1099/0022-1317-35-3-555. [DOI] [PubMed] [Google Scholar]
  20. O'Farrell P.H. High-resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 1975;250:4007–4021. [PMC free article] [PubMed] [Google Scholar]
  21. Pringle C.R. Evidence of genetic recombination in foot-and-mouth disease virus. Virology. 1965;25:48–54. doi: 10.1016/0042-6822(65)90250-3. [DOI] [PubMed] [Google Scholar]
  22. Sangar D.V. The replication of picornaviruses. J. Gen. Virol. 1979;45:1–13. doi: 10.1099/0022-1317-45-1-1. [DOI] [PubMed] [Google Scholar]
  23. Sangar D.V., Black D.N., Rowlands D.J., Harris T.J.R., Brown F. Location of the initiation site for protein synthesis on foot-and-mouth disease virus RNA by in vitro translation of defined fragments of the RNA. J. Virol. 1980;33:59–68. doi: 10.1128/jvi.33.1.59-68.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Saunders K., King A.M.Q. Guanidine-resistant mutants of aphthovirus induce the synthesis of an altered non-structural polypeptide P34. J. Virol. 1982 doi: 10.1128/jvi.42.2.389-394.1982. in press. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Scholtissek C., Rohde W., Von Hoyningen V.V., Rott R. On the origin of the human influenza virus subtypes H2N2 and H3N2. Virology. 1978;87:13–20. doi: 10.1016/0042-6822(78)90153-8. [DOI] [PubMed] [Google Scholar]
  26. Sugiyama K., Bishop D.H.L., Roy P. Analyses of the genomes of bluetongue viruses recovered in the United States. Virology. 1981;114:210–217. doi: 10.1016/0042-6822(81)90266-x. [DOI] [PubMed] [Google Scholar]

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